4.1.1.1 additional information - additional information - 114299, 651094 4.1.1.1 additional information - additional information binding affinity of CoA is 0.11 mM 723667 4.1.1.1 additional information - additional information kinetic data 649662, 650049, 650541 4.1.1.1 additional information - additional information kinetic data, kinetic model 650671 4.1.1.1 additional information - additional information kinetic data, pH-dependence of steady-state kinetic parameters 649910 4.1.1.1 additional information - additional information kinetic data, pH-dependence of steady-state kinetic parameters of wild-type, W412F and W412A mutant PDC 649851 4.1.1.1 additional information - additional information kinetic model, kinetic data 650080 4.1.1.1 additional information - additional information kinetic model, Km values for different conformations of wild-type enzyme at different pH values between pH 4.5 and 6.5 650144 4.1.1.1 additional information - additional information kinetic parameters for carboligase reactions of wild-type and mutant YPDC 650079 4.1.1.1 additional information - additional information kinetic studies 651124 4.1.1.1 additional information - additional information kinetics analysis of the wild-type enzyme with beta-hydroxypyruvate as substrate in the decarboxylation reaction 678576 4.1.1.1 additional information - additional information pH-dependent kinetic data of wild-type, C221E/C222A and C221A/C222A double mutant YPDC 650152 4.1.1.1 additional information - additional information pre-steady-state and steady-state kinetics of recombinant wild-type and mutant enzymes, overview 678162 4.1.1.1 additional information - additional information steady-state kinetic analysis, overview. The v/[S] plots display negative cooperativity and hence deviate from Michaelis-Menten kinetics in just the opposite way 727473 4.1.1.1 additional information - additional information steady-state kinetic parameters for beta-hydroxypyruvate 678579 4.1.1.1 additional information - additional information the isozyme shows sigmoidal kinetics with a Hill coefficient of 1.8 679569 4.1.1.1 additional information - additional information the recombinant enzyme expressed in Geobacillus thermoglucosidasius shows normal Michaelis-Menten kinetics with pyruvate 726787 4.1.1.1 additional information - additional information transient state, pre-steady-state, and steady-state complex formations of substrate/intermediate and thiamine diphosphate cofactor and of kinetics of wild-type and mutant enzymes, overview 727679 4.1.1.1 additional information - additional information values for several C-terminal deletion mutants, kinetic model of the catalytic cycle 650009 4.1.1.1 0.02 - pyruvate mutant enzyme A287G, at pH 6.0 and 30°C 740088 4.1.1.1 0.042 - pyruvate in 100 mM MES buffer (pH 5.6), 5 mM MgCl2, 1 mM dithiothreitol, and 1 mM thiamine diphosphate, at 25°C 700675 4.1.1.1 0.06 - pyruvate at pH 5.0 and 25°C 735870 4.1.1.1 0.06 - pyruvate pH 5.0, 25°C 735870 4.1.1.1 0.1 - pyruvate mutant enzyme S311A, at pH 6.0 and 30°C 740088 4.1.1.1 0.1 2 pyruvate pH 5.0, 55°C, recombinant enzyme 726787 4.1.1.1 0.117 - pyruvate - 114313 4.1.1.1 0.15 - pyruvate mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate 714220 4.1.1.1 0.18 0.2 pyruvate 30°C, E473D mutant PDC 649662 4.1.1.1 0.2 - 2-ketohexanoic acid mutant I472A, pH 6.5, 30°C 666806 4.1.1.1 0.24 - pyruvate pH 6, 30°C 651124 4.1.1.1 0.25 - pyruvate 30°C, D27E mutant PDC 649662 4.1.1.1 0.25 - pyruvate pH 8.4, 80°C 726840 4.1.1.1 0.3 - pyruvate - 114282 4.1.1.1 0.3 - pyruvate mutant enzyme H92F, at pH 6.0 and 30°C 740088 4.1.1.1 0.31 - pyruvate wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate 714220 4.1.1.1 0.38 - pyruvate - 114310 4.1.1.1 0.4 - pyruvate mutant enzyme C221A/C222A, at pH 6.5 and 10°C 740088 4.1.1.1 0.4 - pyruvate mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate 714220 4.1.1.1 0.4 - pyruvate pH 6.0 114283 4.1.1.1 0.43 0.48 pyruvate 30°C, D27N mutant PDC 649662 4.1.1.1 0.5 - 2-ketohexanoic acid mutant I472A/I476F, pH 6.5, 30°C 666806 4.1.1.1 0.5 - pyruvate - 114299 4.1.1.1 0.5 - pyruvate mutant enzyme E50D 114285 4.1.1.1 0.5 - pyruvate wild type enzyme, at pH 6.0 and 30°C 740088 4.1.1.1 0.52 - pyruvate wild type enzyme and mutant enzyme E449D 114299 4.1.1.1 0.55 - pyruvate histidine buffer, enzyme from healthy tissue 114289 4.1.1.1 0.6 - pyruvate - 114288 4.1.1.1 0.6 - pyruvate at pH 6.0 and 25°C 735870 4.1.1.1 0.62 - pyruvate pH 6.2, 25°C 663644 4.1.1.1 0.66 0.68 pyruvate 30°C, wild-type PDC 649662 4.1.1.1 0.68 - pyruvate 30°C, wild-type PDC 650009 4.1.1.1 0.71 - pyruvate mutant enzyme H114Q 114299 4.1.1.1 0.72 - pyruvate per subunit, pH 6.0, 30°C, mutant E51A 727679 4.1.1.1 0.74 - pyruvate pH 6.0, 55°C, native enzyme 726787 4.1.1.1 0.75 - pyruvate - 114307 4.1.1.1 0.8 - pyruvate - 691578 4.1.1.1 0.8 - pyruvate isoform PDC I, pH 6.0, 30°C 665422 4.1.1.1 0.85 - pyruvate histidine buffer, enzyme from diseased tissue 114289 4.1.1.1 0.86 - pyruvate mutant enzyme W487L 114299 4.1.1.1 0.9 - pyruvate isoform PDC II, pH 6.0, 30°C 665422 4.1.1.1 0.92 - pyruvate pH 8.4, 80°C 736379 4.1.1.1 0.95 - pyruvate mutant enzyme D440E and mutant enzyme N467D 114299 4.1.1.1 0.97 - pyruvate mutant enzyme F496I 114299 4.1.1.1 1.04 1.17 pyruvate 30°C, E473Q mutant PDC 649662 4.1.1.1 1.06 - pyruvate mutant enzyme F496H 114299 4.1.1.1 1.1 - pyruvate mutant L112A, pH 6.5, 30°C 736040 4.1.1.1 1.1 - pyruvate per subunit, pH 6.0, 30°C, wild-type enzyme 727679 4.1.1.1 1.1 - pyruvate pH and temperature not specified in the publication 723667 4.1.1.1 1.1 - pyruvate wild type, pH 6.5, 30°C 666806 4.1.1.1 1.1 - pyruvate wild-type, pH 6.5, 30°C 736040 4.1.1.1 1.2 - pyruvate at pH 7.0 and 25°C 735870 4.1.1.1 1.2 - pyruvate pH 6.5, 55°C, recombinant enzyme 726787 4.1.1.1 1.2 - pyruvate pH 7.0, 25°C 735870 4.1.1.1 1.25 - pyruvate pH 6.2, 25°C, presence of 0.1 M NaCl 663644 4.1.1.1 1.27 - 2-oxoisopentanoate - 114313 4.1.1.1 1.33 - pyruvate mutant enzyme V111A 114299 4.1.1.1 1.4 - pyruvate pH 8.4, 80°C 736379 4.1.1.1 1.47 - pyruvate per subunit, pH 6.0, 30°C, mutant C221E/C222A 727679 4.1.1.1 1.66 - pyruvate per subunit, pH 6.0, 30°C, mutant D28A 727679 4.1.1.1 1.7 - 2-keto-4-methylhexanoic acid mutant I472A, pH 6.5, 30°C 666806 4.1.1.1 1.7 - pyruvate phosphate buffer, enzyme from healthy tissue 114289 4.1.1.1 1.73 - pyruvate pH 6, 25°C, sigmoidal dependence of the reaction rate from substrate concentration, Hill coefficient 2.10 650447 4.1.1.1 1.79 - pyruvate per subunit, pH 6.0, 30°C, mutant E91D 727679 4.1.1.1 1.8 - benzoylformate mutant I472A, pH 6.5, 30°C 666806 4.1.1.1 1.9 - 2-oxoisovalerate pH 6.5, 37°C 664985 4.1.1.1 2.2 - 2-ketobutyric acid in 100 mM MES buffer (pH 5.6), 5 mM MgCl2, 1 mM dithiothreitol, and 1 mM thiamine diphosphate, at 25°C 700675 4.1.1.1 2.29 - pyruvate - 691578 4.1.1.1 2.3 - pyruvate phosphate buffer, enzyme from diseased tissue 114289 4.1.1.1 2.3 6 pyruvate pH 6.0, 37°C 727559 4.1.1.1 2.42 - 2-oxoisocaproate pH 7.0, 30°C 735821 4.1.1.1 2.5 - 2-ketopentanoic acid mutant I472A, pH 6.5, 30°C 666806 4.1.1.1 2.5 - pyruvate in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate 705199 4.1.1.1 2.6 - pyruvate mutant I476F, pH 6.5, 30°C 666806 4.1.1.1 2.8 - pyruvate in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate 705199 4.1.1.1 2.8 - pyruvate pH 6.5, potassium MES buffer, two affinities for pyruvate, sigmoidal kinetics 653155 4.1.1.1 2.8 - pyruvate pH 7.0, 55°C, recombinant enzyme 726787 4.1.1.1 2.86 - 2-Ketobutyrate 30°C, wild-type PDC 650009 4.1.1.1 3 - pyruvate - 114281 4.1.1.1 3.02 - pyruvate per subunit, pH 6.0, 30°C, mutant E477Q 727679 4.1.1.1 3.4 - pyruvate mutant N482D, pH 6.5, 30°C 736040 4.1.1.1 3.6 - pyruvate at pH 6.5 and 50°C 746673 4.1.1.1 3.7 - 2-keto-4-methylhexanoic acid mutant I472A/I476F, pH 6.5, 30°C 666806 4.1.1.1 3.9 - pyruvate pH 6.5, 35C, isozyme 1 679569 4.1.1.1 4.4 - benzoylformate mutant I472A/I476F, pH 6.5, 30°C 666806 4.1.1.1 4.4 - pyruvate - 114287 4.1.1.1 4.5 - pyruvate pH 6.5, 35°C, isozyme 2 679569 4.1.1.1 4.7 - 2-ketobutanoic acid wild type, pH 6.5, 30°C 666806 4.1.1.1 4.7 - pyruvate mutant I476L, pH 6.5, 30°C 736040 4.1.1.1 6.7 - 2-ketobutanoic acid mutant I472A, pH 6.5, 30°C 666806 4.1.1.1 6.8 - pyruvate mutant I476A, pH 6.5, 30°C 736040 4.1.1.1 7.6 - 2-ketopentanoic acid wild type, pH 6.5, 30°C 666806 4.1.1.1 7.73 - 2-oxoisovalerate pH 7.0, 30°C 735821 4.1.1.1 7.8 - pyruvate mutant I472A, pH 6.5, 30°C 666806 4.1.1.1 8.9 - pyruvate mutant I476V, pH 6.5, 30°C 736040 4.1.1.1 9.1 - pyruvate mutant I472A, pH 6.5, 30°C 736040 4.1.1.1 10 - pyruvate pH 6.5, sodium hydrogen maleate buffer, two affinities for pyruvate, sigmoidal kinetics 653155 4.1.1.1 11 - 2-ketopentanoic acid mutant I472A/I476F, pH 6.5, 30°C 666806 4.1.1.1 12.2 - pyruvate - 114295 4.1.1.1 12.7 - 2-ketohexanoic acid wild type, pH 6.5, 30°C 666806 4.1.1.1 12.9 - 2-ketovalerate 30°C, wild-type PDC 650009 4.1.1.1 12.9 - 2-oxomethylvalerate pH 7.0, 30°C 735821 4.1.1.1 14.9 - pyruvate per subunit, pH 6.0, 30°C, mutant E51Q 727679 4.1.1.1 16 - pyruvate mutant enzyme H310F, at pH 6.0 and 30°C 740088 4.1.1.1 18 - Pyruvic acid pH 6.0, 30°C, recombinant mutant V461I 678576 4.1.1.1 22 - Pyruvic acid pH 6.0, 30°C, recombinant mutant M538W 678576 4.1.1.1 23.1 - pyruvate per subunit, pH 6.0, 30°C, mutant E51D 727679 4.1.1.1 31.5 - pyruvate per subunit, pH 6.0, 30°C, mutant E51N 727679 4.1.1.1 34 - Pyruvic acid pH 6.0, 30°C, recombinant mutant S286Y 678576 4.1.1.1 40 - 2-keto-4-methylhexanoic acid and above, wild type, pH 6.5, 30°C 666806 4.1.1.1 50 - 2-ketobutanoic acid mutant I472A/I476F, pH 6.5, 30°C 666806 4.1.1.1 50 - pyruvate mutant I472A/I476F, pH 6.5, 30°C 666806 4.1.1.1 65 - Pyruvic acid pH 6.0, 30°C, recombinant mutant F381W 678576