3.6.1.17 additional information - additional information kinetics strongly depend on pH and cation, overview 687119 3.6.1.17 0.0006 - 5',5''-diadenosine tetraphosphate wild-type, pH 7.5, 22°C 733450 3.6.1.17 0.0007 - P1,P4-bis(5'-adenosyl) tetraphosphate - 209906 3.6.1.17 0.0008 - 5',5''-diadenosine tetraphosphate mutant P133F, pH 7.5, 22°C 733450 3.6.1.17 0.001 - P1,P4-bis(5'-guanosyl) tetraphosphate - 209901 3.6.1.17 0.0019 - P1,P4-bis(5'-adenosyl) tetraphosphate - 209905 3.6.1.17 0.002 - diadenosine 5',5'-P1,P4-tetraphosphate - 697896 3.6.1.17 0.002 - P1,P4-bis(5'-adenosyl) tetraphosphate - 209895, 209896 3.6.1.17 0.002 - P1,P4-bis(5'-guanosyl) tetraphosphate - 209895, 209896 3.6.1.17 0.0025 - diadenosine 5',5'-P1,P4-tetraphosphate - 697896 3.6.1.17 0.003 - 5',5''-diadenosine tetraphosphate mutant P133A, pH 7.5, 22°C 733450 3.6.1.17 0.0041 - Ap4A mutant enzyme H38K 656173 3.6.1.17 0.005 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MnCl2, pH 7.5, 22°C 733346 3.6.1.17 0.005 - P1,P4-bis(5'-adenosyl) tetraphosphate - 209901 3.6.1.17 0.005 - P1,P4-bis(5'-guanosyl) tetraphosphate - 209894, 209896 3.6.1.17 0.0053 - Ap4A mutant enzyme E52Q 656173 3.6.1.17 0.0058 - Ap4A mutant enzyme E103Q 656173 3.6.1.17 0.0058 - Ap4A mutant enzyme H38G 656173 3.6.1.17 0.007 - Ap4A pH 7.8, 25°C 654817 3.6.1.17 0.007 - diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.007 - P1,P4-bis(5'-adenosyl) tetraphosphate - 209900 3.6.1.17 0.0073 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MnCl2, pH 7.5, 22°C 733346 3.6.1.17 0.0079 - Ap4A mutant enzyme E56Q 656173 3.6.1.17 0.0088 - Ap4A wild-type enzyme 656173 3.6.1.17 0.009 - 5',5'-diadenosine tetraphosphate pH 6.5, 1 mm Zn2+ 687119 3.6.1.17 0.0093 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MnCl2, pH 7.5, 22°C 733346 3.6.1.17 0.01 - P1,P4-bis(5'-adenosyl) tetraphosphate in presence of Mg2+ 209908 3.6.1.17 0.01 - P1,P4-bis(5'-uridyl) tetraphosphate - 209896 3.6.1.17 0.011 - Ap4A mutant enzyme Y27A 656173 3.6.1.17 0.011 - P1,P4-bis(5'-uridyl) tetraphosphate - 209896 3.6.1.17 0.012 - 5',5'-diadenosine tetraphosphate pH 7.5, 20 mM Mg2+ 687119 3.6.1.17 0.013 - Ap4A mutant enzyme K36M 656173 3.6.1.17 0.015 - 5',5'-diadenosine hexaphosphate pH 7.5, 20 mM Mg2+ 687119 3.6.1.17 0.015 - Ap4A mutant enzyme K79M 656173 3.6.1.17 0.016 - Ap4A mutant enzyme Y27D 656173 3.6.1.17 0.016 - P1,P4-bis(5'-xanthosyl) tetraphosphate - 209896 3.6.1.17 0.018 - P1,P4-bis(5'-xanthosyl) tetraphosphate - 209896 3.6.1.17 0.02 - Ap4A mutant enzyme K81M 656173 3.6.1.17 0.033 - Ap4A mutant enzyme Y76A/Y121A 656173 3.6.1.17 0.038 - Ap4A pH 7.4, 37°C 657213 3.6.1.17 0.04 - Ap6A pH 7.4, 37°C 657213 3.6.1.17 0.04 - P1,P4-bis(5'-adenosyl) tetraphosphate in presence of Zn2+ 209908 3.6.1.17 0.054 - P1,P4-bis(5'-adenosyl) tetraphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C 710715 3.6.1.17 0.059 - Ap4A mutant enzyme Y76A 656173 3.6.1.17 0.073 - Ap4A mutant enzyme H31A 656173 3.6.1.17 0.075 - Ap4A mutant enzyme Y121A 656173 3.6.1.17 0.089 - P1,P6-bis(5'-adenosyl) hexaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C 710715 3.6.1.17 0.098 - Ap5A pH 7.4, 37°C 657213 3.6.1.17 0.0986 - 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.11 - Ap4A mutant enzyme H31V 656173 3.6.1.17 0.1143 - dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.12 - Ap4A pH 8.0, 25°C, mutant enzyme E55Q fusion protein containing and N-terminal extension of Gly-Pro-Leu-Gly-Ser 654489 3.6.1.17 0.14 - Ap4A mutant enzyme K83M 656173 3.6.1.17 0.14 - P1,P4-bis(5'-adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ 733872 3.6.1.17 0.182 - dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.183 - P1,P5-bis(5'-adenosyl) pentaphosphate in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C 710715 3.6.1.17 0.19 - P1,P5-bis(5'-adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ 733872 3.6.1.17 0.326 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MgCl2, pH 7.5, 22°C 733346 3.6.1.17 0.333 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MgCl2, pH 7.5, 22°C 733346 3.6.1.17 0.3846 - 2-oxo-dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C 719339 3.6.1.17 0.407 - diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MgCl2, pH 7.5, 22°C 733346 3.6.1.17 0.5 - Ap4A pH 8.0, 25°C, mutant enzyme E122Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 0.5 - Ap4A pH 8.0, 25°C, mutant enzyme R54Q fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 0.96 - Ap4A pH 8.0, 25°C, mutant enzyme E58D fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 1.03 - P1,P4-bis(5'-guanosyl) tetraphosphate - 209893 3.6.1.17 1.4 - Ap4A pH 8.0, 25°C, mutant enzyme E125Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser 654489 3.6.1.17 2 - Ap4A pH 8.0, 25°C, mutant enzyme E58Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 2.5 - Ap4A pH 8.0, 25°C, wild-type enzyme 654489 3.6.1.17 5.9 - Ap4A pH 8.0, 25°C, mutant enzyme E59Q, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489 3.6.1.17 10 - Ap4A pH 8.0, 25°C, mutant enzyme E59D, fusion protein containing and N-terminal extension Gly-Pro-Leu-Gly-Ser-Pro-Asn-Cys 654489