3.4.23.20 additional information - additional information - 30592 3.4.23.20 additional information - additional information overview: Km values for the hydrolysis of the peptides of the series Ac-(Ala)m-Lys-(4-nitro)Phe-(Ala)n amide and of the series Ac-Xaa-Lys-(4-nitro)Phe-Ala-Ala amide at the pH optimum for kcat, at pH 5.5 and pH 6.0 30588 3.4.23.20 additional information - additional information substrate binding subsite kinetics 668813 3.4.23.20 0.004 - Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester pH 5.5 30599 3.4.23.20 0.005 - Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu - 30589 3.4.23.20 0.0076 - Trypsinogen - 30585 3.4.23.20 0.01 - Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester pH 3.5 30599 3.4.23.20 0.017 - Ac-Val-Lys-(4-nitro)Phe-Ala amide - 30588 3.4.23.20 0.07 - Ac-Gly-Lys-(4-nitro)Phe-Ala-Ala amide pH 4.5 30588 3.4.23.20 0.075 - Ac-Ala-Ala-Ala-Lys-(4-nitro)-Phe-Ala-Ala amide pH 5.5 (pH-optimum for kcat) 30588 3.4.23.20 0.078 - Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide pH 5.5 30589 3.4.23.20 0.21 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.22 - Ac-Lys 4-nitrophenyl amide - 30599 3.4.23.20 0.25 - Ac-Ala-Ala-Lys-(4-nitro)Phe amide pH 4.5 (pH optimum for kcat) 30588 3.4.23.20 0.3 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.32 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.35 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.37 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, and mutant T219S 653829 3.4.23.20 0.39 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291S 653829 3.4.23.20 0.4 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.4 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.4 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.41 - Ac-Ala-Lys-(4-nitro)Phe amide pH 4.3 (optimum for kcat) 30588 3.4.23.20 0.41 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.42 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.44 - Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide - 30599 3.4.23.20 0.45 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.45 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.45 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.46 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.46 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.46 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.48 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.48 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.48 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.5 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.5 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.5 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.5 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.51 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219S 653829 3.4.23.20 0.54 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.55 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291G 653829 3.4.23.20 0.55 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.57 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.59 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291V 653829 3.4.23.20 0.59 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.59 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, wild-type Pencillopepsin JT-2 653829 3.4.23.20 0.6 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.6 - Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.6 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.6 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.6 - Ac-Lys-(4-nitro)Phe amide pH 3.8-4.2 (optimum for kcat) 30588 3.4.23.20 0.6 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219G and T219S 653829 3.4.23.20 0.65 - Ac-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.68 - Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T291A 653829 3.4.23.20 0.71 - Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V and T219A 653829 3.4.23.20 0.74 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.75 - Ac-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.77 - Ac-Lys-(NO2)Phe-Ala-Ala-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.8 - Ac-Ala-Ala-Lys-(NO2)Phe-amide pH 5.0, 25°C, mutant T219A 653829 3.4.23.20 0.8 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219V 653829 3.4.23.20 0.83 - Ac-Ala-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219G 653829 3.4.23.20 0.83 - Ac-Lys-(NO2)Phe-Ala-amide pH 5.0, 25°C, mutant T219A 653829