3.4.22.44 0.05 - WDGGEVAHQAGESV recombinant S129D NIa-Pro mutant, pH 8.5, 25°C 718449 3.4.22.44 0.06 - WDGGEVAHQAGESV recombinant S129A NIa-Pro mutant, pH 8.5, 25°C 718449 3.4.22.44 0.069 - Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg 27 kDa full length protease 137279 3.4.22.44 0.082 - TETVRFQSGTRR recombinant wild-type TVMV protease, pH 7.0, 30°C 718366 3.4.22.44 0.108 - TETVRFQSGTRR recombinant mutant TVMV1-217 protease, pH 7.0, 30°C 718366 3.4.22.44 0.15 - WDGGEVAHQAGESV recombinant S129D NIa-Pro mutant in presence of the VPg domain, pH 8.5, 25°C 718449 3.4.22.44 0.216 - acetyl-Asn-Asn-Val-Arg-Phe-Gln-Ser-Leu-amide - 137278 3.4.22.44 0.217 - acetyl-Arg-Glu-Thr-Val-Arg-Phe-Gln-Ser-Asp-amide - 137278 3.4.22.44 0.271 - Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg truncated protease 137279 3.4.22.44 0.44 - WDGGEVAHQAGESV recombinant wild-type NIa-Pro, pH 8.5, 25°C 718449 3.4.22.44 0.45 - WDGGEVAHQAGESV recombinant E191A VPg-Pro mutant, pH 8.5, 25°C 718449 3.4.22.44 0.51 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease, pH 7.0, 12°C 137277 3.4.22.44 0.52 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease, pH 7.4, 12°C 137277 3.4.22.44 0.62 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease, pH 8.0, 12°C 137277 3.4.22.44 0.69 - WDGGEVAHQAGESV recombinant W143A NIa-Pro mutant, pH 8.5, 25°C 718449 3.4.22.44 1.14 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease, pH 7.0, 25°C 137277 3.4.22.44 1.2 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease, pH 7.4, 25°C 137277 3.4.22.44 1.67 - WDGGEVAHQAGESV recombinant VPg-Pro, pH 8.5, 25°C 718449 3.4.22.44 2.22 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease 137276 3.4.22.44 2.64 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 25 kDa protease 137276 3.4.22.44 2.83 - acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide 27 kDa full length protease, pH 8.0, 25°C 137277 3.4.22.44 11.4 - WDGGEVAHQAGESV recombinant W143A NIa-Pro mutant in presence of the VPg domain, pH 8.5, 25°C 718449