2.5.1.19 additional information - additional information - 658759 2.5.1.19 additional information - additional information comparison of kinetic parameters from a variety of organism 638183 2.5.1.19 additional information - additional information kinetic parameters of native and diethyldicarbonate-inactivated enzyme 638177 2.5.1.19 additional information - additional information kinetic study 638176, 638181 2.5.1.19 additional information - additional information kinetics, wild-type and mutant enzymes 658298 2.5.1.19 additional information - additional information small residue substitutions for Pro101 do not alter the KM for phosphoenolpyruvate and shikimate 3-phosphate 693010 2.5.1.19 additional information - additional information stopped-flow kinetics 658112 2.5.1.19 additional information - phosphoenolpyruvate KM/KI: 13, mutant P101S 693010 2.5.1.19 additional information - phosphoenolpyruvate KM/KI: 150 693010 2.5.1.19 additional information - phosphoenolpyruvate KM/KI: 2.3, mutant P101L 693010 2.5.1.19 additional information - phosphoenolpyruvate KM/KI: 4.2, mutant P101A 693010 2.5.1.19 additional information - phosphoenolpyruvate KM/KI: 7.5, mutant P101G 693010 2.5.1.19 0.00025 - 5-enolpyruvylshikimate 3-phosphate - 638173 2.5.1.19 0.00036 - 3-phosphoshikimate - 638173 2.5.1.19 0.001 - 5-enolpyruvylshikimate 3-phosphate - 638183 2.5.1.19 0.0018 - phosphate - 638173 2.5.1.19 0.0018 - phosphoenolpyruvate pH 7.0, 30°C, mutant StaroA-T42M 658298 2.5.1.19 0.0019 - 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, mutant enzyme G99A 682499 2.5.1.19 0.00195 - 5-enolpyruvylshikimate 3-phosphate reverse reaction, in the presence of 50 mM KCl 638202 2.5.1.19 0.002 - 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, wild-type enzyme 682499 2.5.1.19 0.0024 - phosphoenolpyruvate pH 7.0, 30°C, mutant EcaroA-T42M 658298 2.5.1.19 0.0025 - 3-phosphoshikimate recombinant overproducing strain 638173, 638175 2.5.1.19 0.0025 - 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, mutant enzyme L104P 682499 2.5.1.19 0.0027 - phosphoenolpyruvate - 638173 2.5.1.19 0.003 - 5-enolpyruvylshikimate 3-phosphate recombinant overproducing strain 638173, 638175 2.5.1.19 0.0032 0.0036 3-phosphoshikimate - 638176 2.5.1.19 0.0032 0.0036 3-phosphoshikimate wild-type enzyme 638175 2.5.1.19 0.0038 - phosphoenolpyruvate glyphosate-sensitive strain 638210 2.5.1.19 0.0052 - 5-enolpyruvylshikimate 3-phosphate - 638184 2.5.1.19 0.0052 - phosphoenolpyruvate - 638184 2.5.1.19 0.0052 - phosphoenolpyruvate cosubstrate 5-enolpyruvylshikimate 3-phosphate 638173 2.5.1.19 0.0056 - phosphate 25°C, pH 7.0, wild-type enzyme 682499 2.5.1.19 0.007 - 3-phosphoshikimate - 638187 2.5.1.19 0.007 - 5-enolpyruvylshikimate 3-phosphate H385N mutant enzyme 638200 2.5.1.19 0.007 - phosphoenolpyruvate glyphosate-resistant strain 638210 2.5.1.19 0.0073 - phosphate 25°C, pH 7.0, mutant enzyme G99A 682499 2.5.1.19 0.0077 - 3-phosphoshikimate - 638173, 638184 2.5.1.19 0.008 - phosphoenolpyruvate - 638187 2.5.1.19 0.009 - phosphoenolpyruvate glyphosate-sensitive enzyme 638180 2.5.1.19 0.011 - 5-enolpyruvylshikimate 3-phosphate wild-type enzyme 638200 2.5.1.19 0.011 - phosphoenolpyruvate wild type enzyme, at 28°C, pH not specified in the publication 739266 2.5.1.19 0.0113 - phosphoenolpyruvate expressed in Escherichia coli 694557 2.5.1.19 0.015 - phosphoenolpyruvate wild-type enzyme 638175 2.5.1.19 0.016 - phosphoenolpyruvate recombinant overproducing strain 638173, 638175 2.5.1.19 0.017 - phosphoenolpyruvate in the presence of glyphosate 638182 2.5.1.19 0.018 - 3-phosphoshikimate C-terminal His6-tagged AroA, 50 mM KCl 702295 2.5.1.19 0.0181 - phosphoenolpyruvate - 694557 2.5.1.19 0.0199 - phosphoenolpyruvate wild-type enzyme 682400 2.5.1.19 0.02 - shikimate 3-phosphate - 638177 2.5.1.19 0.021 0.023 phosphoenolpyruvate - 638176 2.5.1.19 0.02167 - phosphoenolpyruvate at pH 7.0 and 30°C 758974 2.5.1.19 0.022 - phosphoenolpyruvate in the presence of 100 mM NH4Cl 638202 2.5.1.19 0.0223 - phosphoenolpyruvate pH 7.0, 30°C, wild-type EcaroA 658298 2.5.1.19 0.023 - phosphoenolpyruvate in the presence of 1 mM NH4Cl and 100 mM KCl 638202 2.5.1.19 0.0248 - phosphate 25°C, pH 7.0, mutant enzyme L104P 682499 2.5.1.19 0.025 - phosphoenolpyruvate - 638177 2.5.1.19 0.025 - phosphoenolpyruvate mutant N130W 704769 2.5.1.19 0.028 - phosphoenolpyruvate at pH 8.0 and 30°C 738881 2.5.1.19 0.028 - phosphoenolpyruvate isoenzyme II 638198 2.5.1.19 0.029 - shikimate 3-phosphate isoenzyme II 638198 2.5.1.19 0.03 - phosphoenolpyruvate at pH 8.0 and 40°C 739671 2.5.1.19 0.031 - shikimate 3-phosphate in the presence of 100 mM NH4Cl 638202 2.5.1.19 0.032 - phosphoenolpyruvate mutant enzyme A187T, at 28°C, pH not specified in the publication 739266 2.5.1.19 0.032 - phosphoenolpyruvate wild-type 704769 2.5.1.19 0.033 - phosphoenolpyruvate isoenzyme I 638198 2.5.1.19 0.033 - shikimate 3-phosphate isoenzyme I 638198 2.5.1.19 0.034 - phosphoenolpyruvate - 638183 2.5.1.19 0.035 - 3-phosphoshikimate 50 mM KCl, mutant E341M 702295 2.5.1.19 0.035 - phosphoenolpyruvate mutant N130S 704769 2.5.1.19 0.04 - 3-phosphoshikimate 50 mM KCl, mutant E341Q 702295 2.5.1.19 0.04 - phosphoenolpyruvate C-terminal His6-tagged AroA, 50 mM KCl 702295 2.5.1.19 0.0436 - phosphoenolpyruvate pH 7.0, 30°C, wild-type StaroA 658298 2.5.1.19 0.045 - 3-phosphoshikimate glyphosate-sensitive enzyme 638180 2.5.1.19 0.045 - phosphoenolpyruvate wild-type 704646 2.5.1.19 0.048 - 3-phosphoshikimate wild-type 704646 2.5.1.19 0.049 - phosphoenolpyruvate mutant enzyme T101A/A187T, at 28°C, pH not specified in the publication 739266 2.5.1.19 0.049 - shikimate 3-phosphate in the presence of 1 mM NH4Cl and 10 mM KCl 638202 2.5.1.19 0.05 - phosphoenolpyruvate 50 mM KCl, mutant E341A 702295 2.5.1.19 0.055 - phosphoenolpyruvate +/-0.0014 mM, mutant N130S in bacterial extact 691436 2.5.1.19 0.055 - phosphoenolpyruvate +/-0.002 mM, bacterial extract 691436 2.5.1.19 0.06 - phosphoenolpyruvate +/-0.006 mM 693010 2.5.1.19 0.06 - shikimate 3-phosphate +/-0.004 mM, mutant P101A 693010 2.5.1.19 0.06 - shikimate 3-phosphate +/-0.006 mM 693010 2.5.1.19 0.06 - shikimate 3-phosphate +/-0.010 mM, mutant P101G 693010 2.5.1.19 0.07 - phosphoenolpyruvate +/-0.005 mM, mutant P101S 693010 2.5.1.19 0.07 - shikimate 3-phosphate H385 mutant enzyme 638200 2.5.1.19 0.071 - 3-phosphoshikimate mutant P101S 704646 2.5.1.19 0.071 - phosphoenolpyruvate mutant P101S 704646 2.5.1.19 0.077 - 3-phosphoshikimate mutant P101S/T97I 704646 2.5.1.19 0.077 - 3-phosphoshikimate mutant T97I 704646 2.5.1.19 0.077 - phosphoenolpyruvate at pH 7.0 and 30°C 758959 2.5.1.19 0.078 - phosphoenolpyruvate wild type enzyme, at pH 7.0 and 28°C 738129 2.5.1.19 0.078 - phosphoenolpyruvate wild-type, pH 7.0, 28°C 723574 2.5.1.19 0.08 - 3-phosphoshikimate 50 mM KCl, mutant E341C 702295 2.5.1.19 0.08 - phosphoenolpyruvate +/-0.005 mM, mutant P101A 693010 2.5.1.19 0.08 - shikimate 3-phosphate +/-0.006 mM, mutant P101S 693010 2.5.1.19 0.081 - phosphoenolpyruvate mutant enzyme T101A, at 28°C, pH not specified in the publication 739266 2.5.1.19 0.083 - 3-phosphoshikimate at pH 8.0 and 40°C 739671 2.5.1.19 0.084 - 3-phosphoshikimate mutant G96A, pH 7.0, 28°C 723574 2.5.1.19 0.085 - phosphoenolpyruvate in the presence of 1 mM NH4Cl and 10 mM KCl 638202 2.5.1.19 0.086 - 3-phosphoshikimate wild-type, pH 7.0, 28°C 723574 2.5.1.19 0.088 - phosphoenolpyruvate wild-type enzyme 638209 2.5.1.19 0.0883 - phosphoenolpyruvate mutant enzyme P106L 682400 2.5.1.19 0.09 - 3-phosphoshikimate 50 mM KCl, mutant H385A 702295 2.5.1.19 0.09 - phosphoenolpyruvate +/-0.010 mM, mutant P101G 693010 2.5.1.19 0.09 - phosphoenolpyruvate wild-type enzyme 638199 2.5.1.19 0.091 - phosphoenolpyruvate in the presence of 10 mM NH4Cl 638202 2.5.1.19 0.0952 - phosphoenolpyruvate +/-0.00573 mM, full-length G2 EPSPS, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay 690523 2.5.1.19 0.0968 - phosphoenolpyruvate +/-0.01101 mM, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from transformed Escherichia coli ER2799, malachite green dye assay 690523 2.5.1.19 0.1 - 3-phosphoshikimate 50 mM KCl, mutant E341A 702295 2.5.1.19 0.1 - phosphoenolpyruvate in presence of 1 mM shikimate 3-phosphate 691087 2.5.1.19 0.1 - phosphoenolpyruvate in the presence of 1 mM NH4Cl 638202 2.5.1.19 0.1 - phosphoenolpyruvate mutant P101S/T97I 704646 2.5.1.19 0.1 - phosphoenolpyruvate wild-type enzyme 638200 2.5.1.19 0.1 - shikimate 3-phosphate +/-0.007 mM, mutant P101L 693010 2.5.1.19 0.106 - phosphoenolpyruvate H385N mutant enzyme 638200 2.5.1.19 0.109 - shikimate 3-phosphate in the presence of 1 mM NH4Cl and 100 mM KCl 638202 2.5.1.19 0.11 - 3-phosphoshikimate C-terminal His6-tagged AroA, 100 mM KCl 702295 2.5.1.19 0.111 - 3-phosphoshikimate at pH 8.0 and 30°C 738881 2.5.1.19 0.118 - shikimate 3-phosphate in the presence of 10 mM NH4Cl 638202 2.5.1.19 0.12 - shikimate 3-phosphate wild-type and G96A mutant enzyme 638209 2.5.1.19 0.135 - shikimate 3-phosphate wild-type enzyme 638200 2.5.1.19 0.14 - 3-phosphoshikimate pH 7.0, recombinant enzyme, in presence of 100 mM KCl 658759 2.5.1.19 0.14 - 3-phosphoshikimate wild-type AroA, 100 mM KCl 702295 2.5.1.19 0.14 - phosphoenolpyruvate glyphosate-resistant enzyme 638180 2.5.1.19 0.144 - 3-phosphoshikimate at pH 7.0 and 30°C 758959 2.5.1.19 0.145 - shikimate 3-phosphate in the presence of 1 mM NH4Cl 638202 2.5.1.19 0.148 - phosphoenolpyruvate mutant G96A, pH 7.0, 28°C 723574 2.5.1.19 0.15 - phosphoenolpyruvate +/-0.020 mM, mutant P101L 693010 2.5.1.19 0.15 - phosphoenolpyruvate C-terminal His6-tagged AroA, 100 mM KCl 702295 2.5.1.19 0.16 - phosphoenolpyruvate wild-type AroA, 100 mM KCl 702295 2.5.1.19 0.16 - shikimate 3-phosphate H385 mutant enzyme 638199 2.5.1.19 0.169 - 3-phosphoshikimate wild type enzyme, at pH 7.0 and 28°C 738129 2.5.1.19 0.17 - phosphoenolpyruvate 50 mM KCl, mutant E341C 702295 2.5.1.19 0.18 - phosphoenolpyruvate pH 7.0, recombinant enzyme, in presence of 100 mM KCl 658759 2.5.1.19 0.19 - 3-phosphoshikimate 50 mM KCl, mutant K22R value above 0.3 702295 2.5.1.19 0.19 - phosphoenolpyruvate 50 mM KCl, mutant D313N 702295 2.5.1.19 0.193 - 3-phosphoshikimate glyphosate-resistant enzyme 638180 2.5.1.19 0.195 - phosphoenolpyruvate pH 7.0, 28°C 723804 2.5.1.19 0.2 - 3-phosphoshikimate 50 mM KCl, mutant D313N 702295 2.5.1.19 0.2 - phosphoenolpyruvate R24D mutant enzyme 638199 2.5.1.19 0.2 - shikimate 3-phosphate R24D mutant enzyme 638199 2.5.1.19 0.21 - 3-phosphoshikimate 50 mM KCl, mutant D313A 702295 2.5.1.19 0.227 - phosphoenolpyruvate mutant enzyme G100A, at pH 7.0 and 28°C 738129 2.5.1.19 0.23 - phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication 721350 2.5.1.19 0.241 - 3-phosphoshikimate mutant T355S, pH 7.0, 28°C 721379 2.5.1.19 0.25 - phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication 721350 2.5.1.19 0.26 - phosphoenolpyruvate at pH 8.0 and 37°C 759163 2.5.1.19 0.275 - 3-phosphoshikimate pH 7.0, 28°C 723145 2.5.1.19 0.28 - phosphoenolpyruvate 50 mM KCl, mutant D313A 702295 2.5.1.19 0.286 - 3-phosphoshikimate wild-type, pH 7.0, 28°C 721379 2.5.1.19 0.29 - phosphoenolpyruvate pH 7.0, 28°C 723145 2.5.1.19 0.292 - phosphoenolpyruvate mutant T355S, pH 7.0, 28°C 721379 2.5.1.19 0.304 - 3-phosphoshikimate pH 7.0, 28°C 723804 2.5.1.19 0.31 - phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication 721350 2.5.1.19 0.316 - phosphoenolpyruvate wild-type, pH 7.0, 28°C 721379 2.5.1.19 0.32 - phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication 721350 2.5.1.19 0.33 - shikimate 3-phosphate wild-type enzyme 638199 2.5.1.19 0.343 - phosphate reverse reaction, in the presence of 50 mM KCl 638202 2.5.1.19 0.37 - phosphoenolpyruvate at pH 8.0 and 22°C 759163 2.5.1.19 0.38 - phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication 721350 2.5.1.19 0.38 - phosphoenolpyruvate mutant T97I 704646 2.5.1.19 0.4 - 3-phosphoshikimate 50 mM KCl, mutant D313C 702295 2.5.1.19 0.43 - shikimate 3-phosphate P105S mutant enzyme 638199 2.5.1.19 0.525 - phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication 721350 2.5.1.19 0.59 - phosphoenolpyruvate P105S mutant enzyme 638199 2.5.1.19 0.7 - 3-phosphoshikimate 50 mM KCl, mutant D313L 702295 2.5.1.19 0.706 - 3-phosphoshikimate mutant enzyme G100A, at pH 7.0 and 28°C 738129 2.5.1.19 0.8 - phosphoenolpyruvate mutant G96A/A183T, in presence of 1 mM shikimate 3-phosphate 691087 2.5.1.19 0.9 - phosphoenolpyruvate 50 mM KCl, mutant E341Q 702295 2.5.1.19 1 - phosphoenolpyruvate H385L mutant enzyme 638199 2.5.1.19 1.2 - phosphate H385N mutant enzyme 638200 2.5.1.19 1.3 - phosphoenolpyruvate 50 mM KCl, mutant D313C 702295 2.5.1.19 1.5 - phosphoenolpyruvate 50 mM KCl, mutant H385A 702295 2.5.1.19 1.6 - phosphoenolpyruvate 50 mM KCl, mutant D313L 702295 2.5.1.19 1.9 - 5-enolpyruvylshikimate 3-phosphate +/-0.3 mM, mutant G99A 694929 2.5.1.19 1.9 - phosphoenolpyruvate 50 mM KCl, mutant E341M 702295 2.5.1.19 2 - 3-phosphoshikimate 50 mM KCl, mutant K22A value above 0.3 702295 2.5.1.19 2.2 - 5-enolpyruvylshikimate 3-phosphate +/-0.5 mM 694929 2.5.1.19 2.5 - 5-enolpyruvylshikimate 3-phosphate +/-0.4 mM, mutant L104P 694929 2.5.1.19 2.5 - phosphate - 638173, 638175 2.5.1.19 2.5 - phosphoenolpyruvate 50 mM KCl, mutant K22R value above 0.3 702295 2.5.1.19 2.8 - phosphoenolpyruvate G96A mutant enzyme 638209 2.5.1.19 3.1 - phosphoenolpyruvate mutant G96A, in presence of 1 mM shikimate 3-phosphate 691087 2.5.1.19 3.7 - phosphate - 638184 2.5.1.19 3.8 - phosphoenolpyruvate mutant A183T, in presence of 1 mM shikimate 3-phosphate 691087 2.5.1.19 4 - phosphate - 638173 2.5.1.19 4.6 - phosphate wild-type enzyme 638200 2.5.1.19 4.762 - phosphoenolpyruvate mutant enzyme L105P, at pH 7.0 and 28°C 738129 2.5.1.19 5.6 - phosphate +/-1.6 694929 2.5.1.19 7.3 - phosphate +/-0.7 mM, mutant G99A 694929 2.5.1.19 22.5 - phosphoenolpyruvate recombinant enzyme, in crude extracts 638203 2.5.1.19 24.8 - phosphate +/-4.8 mM, mutant L104P 694929 2.5.1.19 43.6 - phosphoenolpyruvate recombinant enzyme, in crude extracts 638203