2.3.1.47 additional information - additional information stopped-flow kinetics of enzyme interaction with L-alanine and D-alanine, steady-state kinetics and thermodynamics 674626 2.3.1.47 additional information - additional information the recombinant KAPA synthase obeys Michaelis-Menten kinetics with respect to pimeloyl-CoA and L-Ala 676580 2.3.1.47 0.001 - pimeloyl-CoA - 487048 2.3.1.47 0.0013 - (S)-8-amino-7-oxononanoic acid calculated from the competitive and uncompetitive constants and the substrate concentration determined in inhibitor experiments, 37°C, pH 8.6 702483 2.3.1.47 0.0014 - S-adenosyl-L-methionine calculated from the competitive and uncompetitive constants and the substrate concentration determined in inhibitor experiments, 37°C, pH 8.6 702483 2.3.1.47 0.0015 - pimeloyl-CoA - 487053 2.3.1.47 0.0015 - pimeloyl-CoA pH 7.7, 25°C, recombinant enzyme 674626 2.3.1.47 0.0016 - pimeloyl-CoA pH 7.5, 30°C, recombinant enzyme 676580 2.3.1.47 0.002 - L-alanine - 487053 2.3.1.47 0.01 - pimeloyl-CoA 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C 718696 2.3.1.47 0.01 - pimeloyl-CoA pH 6.0, 60°C 719127 2.3.1.47 0.02 - pimeloyl-CoA pH 6.0, 60°C 719127 2.3.1.47 0.025 - pimeloyl-CoA - 487058 2.3.1.47 0.025 - pimeloyl-CoA pH not specified in the publication, temperature not specified in the publication 719127 2.3.1.47 0.025 - pimeloyl-CoA wild type protein, pH not specified in the publication, temperature not specified in the publication 718696 2.3.1.47 0.03 - pimeloyl-CoA pH 6.0, 60°C 719127 2.3.1.47 0.07 - pimeloyl-CoA - 487056 2.3.1.47 0.09 - L-alanine pH 6.0, 60°C 719127 2.3.1.47 0.2015 - D-alanine pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA 674626 2.3.1.47 0.23 - L-alanine pH 6.0, 60°C 719127 2.3.1.47 0.25 - L-alanine 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C 718696 2.3.1.47 0.4675 - L-alanine pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA 674626 2.3.1.47 0.5 - L-alanine - 487058 2.3.1.47 0.5 - L-alanine pH not specified in the publication, temperature not specified in the publication 719127 2.3.1.47 0.5 - L-alanine wild type protein, pH not specified in the publication, temperature not specified in the publication 718696 2.3.1.47 0.9 - L-alanine - 487056 2.3.1.47 1.4 - L-alanine pH 7.5, 30°C, recombinant enzyme 676580 2.3.1.47 2.28 - L-alanine pH 6.0, 60°C 719127 2.3.1.47 3 - L-alanine - 487048