1.14.99.1 additional information - additional information Michaelis-Menten kinetics 727083 1.14.99.1 additional information - additional information Michaelis-Menten kinetics, overview 715514 1.14.99.1 additional information - additional information the cyclooxygenase reaction shows Michaelis-Menten kinetics over a wide range of oxygen concentrations in the absence of electron donor. Kinetics analysis, overview 714124 1.14.99.1 0.0009 - arachidonate purified isoform PGHS-2, method: coupled, homo-vanilic acid 695888 1.14.99.1 0.001 - arachidonic acid isozyme 1 and 2 438396 1.14.99.1 0.0011 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.0011 - cis-4,7,10,13,16,19-docosahexaenoic acid - 673508 1.14.99.1 0.0012 - cis-5,8,11,14,17-eicosapentaenoic acid - 673508 1.14.99.1 0.0017 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.0017 - cis-5,8,11,14-eicosatetraenoic acid - 673508 1.14.99.1 0.0019 - arachidonate purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.002 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.002 - cis-8,11,14-eicosatrienoic acid - 673508 1.14.99.1 0.0021 - arachidonate native enzyme 658047 1.14.99.1 0.0021 - arachidonate pH 7.2, 30°C, mutant enzyme N382L 659392 1.14.99.1 0.0021 - arachidonate pH 7.2, 30°C, PGHS-2 wild-type enzyme 659392 1.14.99.1 0.0021 - arachidonic acid wild-type, cyclooxygenase activity 672161 1.14.99.1 0.0022 - arachidonate pH 7.2, 30°C, mutant enzyme N383D 659392 1.14.99.1 0.0024 - cis-5,8,11,14-eicosatetraenoic acid - 673508 1.14.99.1 0.0027 - arachidonate purified isoform PGHS-1, method: coupled, homo-vanilic acid 695888 1.14.99.1 0.0027 - cis-7,10,13,16-docosatetraenoic acid - 673508 1.14.99.1 0.0029 - arachidonate pH 7.2, 30°C, mutant enzyme N382D 659392 1.14.99.1 0.00295 - arachidonate pH 8.0, 25°C, COX-2 mutant L531P 715514 1.14.99.1 0.003 - arachidonate purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.003 - cis-5,8,11,14-eicosatetraenoic acid - 673508 1.14.99.1 0.0031 - alpha-linolenic acid - 673508 1.14.99.1 0.0031 - cis-5,8,11,14,17-eicosapentaenoic acid - 673508 1.14.99.1 0.0032 - arachidonate mutant enzyme Y148F 658047 1.14.99.1 0.0033 - cis-4,7,10,13,16,19-docosahexaenoic acid - 673508 1.14.99.1 0.0034 - arachidonic acid mutant Y348F/Y504F, cyclooxygenase activity 672161 1.14.99.1 0.0036 - arachidonate mutant enzyme Y404F 658047 1.14.99.1 0.00365 - arachidonate pH 8.0, 25°C, COX-2 mutant L531A 715514 1.14.99.1 0.0037 - arachidonate pH 7.2, 30°C, mutant enzyme N383H 659392 1.14.99.1 0.0037 - arachidonate purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.0038 - arachidonate mutant enzyme Y348F 658047 1.14.99.1 0.0038 - arachidonic acid mutant Y348F, cyclooxygenase activity 672161 1.14.99.1 0.0041 - arachidonate pH 7.2, 30°C, mutant enzyme N382A 659392 1.14.99.1 0.0042 - arachidonate mutant enzyme Y504F 658047 1.14.99.1 0.0042 - arachidonic acid mutant Y504F, cyclooxygenase activity 672161 1.14.99.1 0.0043 - arachidonate mutant enzyme Y148F/Y348F/Y404F/Y504F 658047 1.14.99.1 0.0045 - arachidonate purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.0045 - arachidonic acid isozyme 1 438402 1.14.99.1 0.0048 - alpha-linolenic acid - 673508 1.14.99.1 0.0048 - gamma-linolenic acid - 673508 1.14.99.1 0.005 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.005 - arachidonic acid isozyme 2 438402 1.14.99.1 0.005 - O2 purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.0051 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.00514 - arachidonate pH 8.0, 25°C, COX-2 wild-type COX-2 and mutant N580A 715514 1.14.99.1 0.0052 - cis-11,14-eicosadienoic acid - 673508 1.14.99.1 0.0055 - cis-8,11,14-eicosatrienoic acid - 673508 1.14.99.1 0.0055 - linoleic acid isozyme PGHS-1, in 100 mM Tris-HCl buffer (pH 7.4), at 24°C 701036 1.14.99.1 0.0055 - O2 purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.0059 - cis-8,11,14-eicosatrienoic acid - 673508 1.14.99.1 0.006 - arachidonic acid - 438397 1.14.99.1 0.0068 - linoleic acid isozyme PGHS-2, in 100 mM Tris-HCl buffer (pH 7.4), at 24°C 701036 1.14.99.1 0.007 - cis-7,10,13,16-docosatetraenoic acid - 673508 1.14.99.1 0.0071 - gamma-linolenic acid - 673508 1.14.99.1 0.0083 - arachidonic acid - 438389 1.14.99.1 0.0083 - N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N383H 659392 1.14.99.1 0.009 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.0091 - cis-11,14-eicosadienoic acid - 673508 1.14.99.1 0.0092 - arachidonate purified isoform PGHS-2, method: direct, O2 695888 1.14.99.1 0.00945 - (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoic acid pH 8.0, 25°C, COX-2 mutant N580A 715514 1.14.99.1 0.01 - arachidonate purified isoform PGHS-2, method: direct, arachidonate 695888 1.14.99.1 0.01 - arachidonate purified isoform PGHS-2, method: direct, trimethyl phosphine oxide 695888 1.14.99.1 0.01 - N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N383D 659392 1.14.99.1 0.01 - O2 purified isoform PGHS-1, method: direct, arachidonate 695888 1.14.99.1 0.0101 - alpha-linolenic acid - 673508 1.14.99.1 0.0102 - arachidonate purified isoform PGHS-1, method: direct, arachidonate 695888 1.14.99.1 0.011 - arachidonate purified isoform PGHS-1, method: coupled, homo-vanilic acid 695888 1.14.99.1 0.011 - O2 purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.013 - cis-5,8,11,14-eicosatetraenoic acid - 673508 1.14.99.1 0.015 - arachidonate purified isoform PGHS-1, method: direct, O2 695888 1.14.99.1 0.015 - arachidonic acid - 438399 1.14.99.1 0.0153 - cis-5,8,11,14,17-eicosapentaenoic acid - 673508 1.14.99.1 0.0156 - N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N382D 659392 1.14.99.1 0.0163 - N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, PGHS-2 wild-type enzyme 659392 1.14.99.1 0.02 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F 658047 1.14.99.1 0.0318 - cis-4,7,10,13,16,19-docosahexaenoic acid - 673508 1.14.99.1 0.0357 - cis-7,10,13,16-docosatetraenoic acid - 673508 1.14.99.1 0.036 - cis-8,11,14-eicosatrienoic acid - 673508 1.14.99.1 0.0368 - (4Z,7Z,10Z,13Z,16Z,19Z)-Docosahexaenoic acid pH 8.0, 25°C, COX-2 mutant N580A 715514 1.14.99.1 0.039 - cis-5,8,11,14,17-eicosapentaenoic acid - 673508 1.14.99.1 0.061 - cis-7,10,13,16-docosatetraenoic acid - 673508 1.14.99.1 0.061 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y404F 658047 1.14.99.1 0.07 - cis-4,7,10,13,16,19-docosahexaenoic acid - 673508 1.14.99.1 0.072 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y348F 658047 1.14.99.1 0.08 - guaiacol pH 8.0, 23°C, wild-type enzyme 659392 1.14.99.1 0.082 - alpha-linolenic acid - 673508 1.14.99.1 0.0854 - N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N382L 659392 1.14.99.1 0.103 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F/Y348F/Y385F/Y404F/Y504F 658047 1.14.99.1 0.138 - trans-5-phenyl-4-pentenyl-1-hydroperoxide native enzyme 658047 1.14.99.1 0.16 - arachidonic acid - 438390 1.14.99.1 0.162 - gamma-linolenic acid - 673508 1.14.99.1 0.29 - guaiacol pH 8.0, 23°C, mutant enzyme N382L 659392 1.14.99.1 0.339 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y385F 658047 1.14.99.1 0.37 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F/Y348F/Y404F/Y504F 658047 1.14.99.1 0.437 - trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y504F 658047 1.14.99.1 1.3 - H2O2 pH 8.0, 23°C, wild-type enzyme 659392 1.14.99.1 5.5 - H2O2 pH 8.0, 23°C, mutant enzyme N382L 659392