1.14.14.3 additional information - additional information affinity and dissociation constants for FMNH2 of wild-type and mutants enzymes, kinetics 658060 1.14.14.3 additional information - additional information detailed reaction and folding kinetics, thermodynamics 657927 1.14.14.3 additional information - additional information enzyme activities in complex formation, kinetics, dissociation constants 657994 1.14.14.3 additional information - additional information kinetics of FMN reductase-luciferase complex formation, overview 689388 1.14.14.3 additional information - additional information kinetics of the FRP:luciferase coupled bioluminescence reaction 671610 1.14.14.3 additional information - additional information kinetics, substrate and cofactor binding 657939 1.14.14.3 additional information - additional information stopped flow spectroscopy 657923 1.14.14.3 additional information - additional information stopped-flow and Michaelis-Menten kinetics of wild-type and mutant enzymes 671985 1.14.14.3 additional information - additional information stopped-flow kinetics of wild-type and mutant enzymes 671978 1.14.14.3 0.0001 - O2 - 348579 1.14.14.3 0.00015 - FMNH alpha subunit 348598 1.14.14.3 0.00018 - FMNH2 luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication 728435 1.14.14.3 0.0002 - FMNH2 pH 7.0, 23°C, recombinant mutant F114S 671978 1.14.14.3 0.0002 - FMNH2 pH 7.0, 23°C, recombinant mutants E328Q and E328H 671985 1.14.14.3 0.0002 - FMNH2 recombinant wild type enzyme, at 22°C, pH not specified in the publication 728435 1.14.14.3 0.0003 - decanal wild type, 50 mM phosphate 348607 1.14.14.3 0.0003 - FMNH2 pH 7.0, 23°C, recombinant mutants E328L and E328A 671985 1.14.14.3 0.0003 - FMNH2 pH 7.0, 23°C, recombinant mutants F46Y, F114A, F117Y, and F114Y 671978 1.14.14.3 0.0003 - FMNH2 recombinant wild-type enzyme, pH 7.0, 25°C 657923 1.14.14.3 0.0004 - decanal recombinant mutant alphaF261S, pH 7.0, 25°C 657923 1.14.14.3 0.0004 - decanal recombinant mutant alphaG275I, pH 7.0, 25°C 657923 1.14.14.3 0.0004 - FMNH2 pH 7.0, 23°C, recombinant mutant E328D 671985 1.14.14.3 0.0004 0.0008 FMNH - 348579, 348591 1.14.14.3 0.00058 - FMNH beta subunit 348598 1.14.14.3 0.0006 - FMNH2 pH 7.0, 23°C, recombinant mutant F49Y 671978 1.14.14.3 0.0006 - FMNH2 pH 7.0, 23°C, recombinant wild-type enzyme 671978, 671985 1.14.14.3 0.0006 - FMNH2 recombinant mutant alphaG284P, pH 7.0, 25°C 657923 1.14.14.3 0.0007 - FMNH2 pH 7.0, 23°C, recombinant mutant F49S 671978 1.14.14.3 0.0008 - FMNH wild type, 10 mM phosphate 348607 1.14.14.3 0.0009 - FMN in the presence of different flavin concentrations, 0.001 mM Fre oxidoreductase, 10 mM decanal, and 0.01 mM NADPH and 0.005 mM luciferase 698992 1.14.14.3 0.001 - decanal recombinant wild-type enzyme, pH 7.0, 25°C 657923 1.14.14.3 0.001 - FMNH2 pH 7.0, 23°C, recombinant mutant F114D 671978 1.14.14.3 0.001 0.01 aldehydes - 348579 1.14.14.3 0.00101 - decanal pH 7.0, 23°C, recombinant mutant F49Y 671978 1.14.14.3 0.00113 - decanal recombinant wild type enzyme, at 22°C, pH not specified in the publication 728435 1.14.14.3 0.0012 0.009 n-decanal depending on buffer system 348571 1.14.14.3 0.0013 - decanal pH 7.0, 23°C, recombinant mutant F46A 671978 1.14.14.3 0.0013 - FMNH2 pH 7.0, 23°C, recombinant mutant F117S 671978 1.14.14.3 0.0013 - FMNH2 pH 7.0, 23°C, recombinant mutant F49D 671978 1.14.14.3 0.0013 - riboflavin in the presence of different flavin concentrations, 0.001 mM Fre oxidoreductase, 10 mM decanal, and 0.01 mM NADPH and 0.005 mM luciferase 698992 1.14.14.3 0.0015 - FMNH2 pH 7.0, 23°C, recombinant mutant A74G 671985 1.14.14.3 0.0015 - FMNH2 pH 7.0, 23°C, recombinant mutant F117A 671978 1.14.14.3 0.0016 - decanal pH 7.0, 23°C, recombinant wild-type enzyme 671978 1.14.14.3 0.0016 - decanal pH 7.0, 23°C, recombinant wild-type enzyme and mutant A74G 671985 1.14.14.3 0.0016 - decanal recombinant mutant alphaF261Y, pH 7.0, 25°C 657923 1.14.14.3 0.00165 - decanal luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication 728435 1.14.14.3 0.0017 - decanal recombinant mutant alphaF261A, pH 7.0, 25°C 657923 1.14.14.3 0.0018 - decanal recombinant mutant alphaG275P, pH 7.0, 25°C 657923 1.14.14.3 0.0018 - FMNH wild type, 300 mM phosphate 348607 1.14.14.3 0.0021 - decanal alphaR107S, 50 mM phosphate 348607 1.14.14.3 0.0022 - decanal alphaR107E, 50 mM phosphate 348607 1.14.14.3 0.0022 - decanal recombinant mutant alphaG275A, pH 7.0, 25°C 657923 1.14.14.3 0.0022 - FMNH2 recombinant mutant alphaG275P, pH 7.0, 25°C 657923 1.14.14.3 0.0023 - decanal pH 7.0, 23°C, recombinant mutant E328Q 671985 1.14.14.3 0.0024 - decanal pH 7.0, 23°C, recombinant mutant F117S 671978 1.14.14.3 0.0024 - decanal pH 7.0, 23°C, recombinant mutant F46Y 671978 1.14.14.3 0.0026 - decanal pH 7.0, 23°C, recombinant mutant F46S 671978 1.14.14.3 0.0027 - decanal pH 7.0, 23°C, recombinant mutant F114Y 671978 1.14.14.3 0.0027 - decanal recombinant mutant alphaG275F, pH 7.0, 25°C 657923 1.14.14.3 0.0027 - FMNH2 pH 7.0, 23°C, recombinant mutant F49A 671978 1.14.14.3 0.003 - decanal pH 7.0, 23°C, recombinant mutants F49D, F117A, and F49A 671978 1.14.14.3 0.0031 - decanal alphaR107A, 50 mM phosphate 348607 1.14.14.3 0.0031 - decanal pH 7.0, 23°C, recombinant mutant F117D 671978 1.14.14.3 0.0032 - decanal pH 7.0, 23°C, recombinant mutant F46D 671978 1.14.14.3 0.0033 - decanal pH 7.0, 23°C, recombinant mutant E328A 671985 1.14.14.3 0.0033 - decanal pH 7.0, 23°C, recombinant mutant F49S 671978 1.14.14.3 0.0037 - decanal recombinant mutant alphaG284P, pH 7.0, 25°C 657923 1.14.14.3 0.0038 - FMNH alphaR107S, 10 mM phosphate 348607 1.14.14.3 0.0039 - FMNH2 pH 7.0, 23°C, recombinant mutant E328F 671985 1.14.14.3 0.0043 - FMNH2 pH 7.0, 23°C, recombinant mutant F46S 671978 1.14.14.3 0.0045 - decanal pH 7.0, 23°C, recombinant mutant E328F 671985 1.14.14.3 0.0046 - decanal recombinant mutant alphaF261D, pH 7.0, 25°C 657923 1.14.14.3 0.0049 - FMNH alphaR107S, 300 mM phosphate 348607 1.14.14.3 0.005 - decanal pH 7.0, 23°C, recombinant mutant E328D 671985 1.14.14.3 0.0051 - decanal pH 7.0, 23°C, recombinant mutant F117Y 671978 1.14.14.3 0.0073 - decanal pH 7.0, 23°C, recombinant mutant F114D 671978 1.14.14.3 0.0075 - FMNH2 recombinant mutant alphaF261D, pH 7.0, 25°C 657923 1.14.14.3 0.008 - decanal wild-type 704579 1.14.14.3 0.0081 - FMNH alphaR107A, 10 mM phosphate 348607 1.14.14.3 0.0083 - decanal pH 7.0, 23°C, recombinant mutant F114S 671978 1.14.14.3 0.0087 - FMNH2 pH 7.0, 23°C, recombinant mutant A74F 671985 1.14.14.3 0.0093 - decanal pH 7.0, 23°C, recombinant mutant F114A 671978 1.14.14.3 0.0095 - decanal pH 7.0, 23°C, recombinant mutant E328H 671985 1.14.14.3 0.0095 - FMNH alphaR107A, 300 mM phosphate 348607 1.14.14.3 0.0097 - decanal pH 7.0, 23°C, recombinant mutant E328L 671985 1.14.14.3 0.01 - decanal mutant K286A 704579 1.14.14.3 0.0105 - decanal mutant K283A 704579 1.14.14.3 0.0114 - FMNH alphaR107E, 300 mM phosphate 348607 1.14.14.3 0.0123 - FMNH2 pH 7.0, 23°C, recombinant mutant F46A 671978 1.14.14.3 0.0148 - FMNH2 pH 7.0, 23°C, recombinant mutant F46D 671978 1.14.14.3 0.0173 - decanal pH 7.0, 23°C, recombinant mutant A74F 671985 1.14.14.3 0.0192 - FMNH2 recombinant mutant alphaF261Y, pH 7.0, 25°C 657923 1.14.14.3 0.0234 - FMNH alphaR107E, 10 mM phosphate 348607 1.14.14.3 0.0281 - FMNH2 recombinant mutant alphaF261A, pH 7.0, 25°C 657923 1.14.14.3 0.0358 - FMNH2 recombinant mutant alphaG275A, pH 7.0, 25°C 657923 1.14.14.3 0.0369 - FMNH2 recombinant mutant alphaF261S, pH 7.0, 25°C 657923 1.14.14.3 0.0411 - FMNH2 recombinant mutant alphaG275F, pH 7.0, 25°C 657923 1.14.14.3 0.052 - FMNH2 pH 7.0, 23°C, recombinant mutant F117D 671978 1.14.14.3 0.0584 - FMNH2 recombinant mutant alphaG275I, pH 7.0, 25°C 657923 1.14.14.3 0.11 - decanal pH 7.0, temperature not specified in the publication 744675 1.14.14.3 0.26 - 4-N,N-(dimethyl)aminonaphthalene-9-N-(11-aldehydedodecyl)-1,8-dicarboximide pH 7.0, temperature not specified in the publication 744675 1.14.14.3 0.72 - 4-N,N-(dimethyl)aminonaphthalene-9-N-(9-aldehyde-decyl)-1,8-dicarboximide pH 7.0, temperature not specified in the publication 744675 1.14.14.3 0.72 - 4-N-(11-aldehyde-dodecyl)-7-N,N-dimethylsulfonic-2,1,3-benzoxadiazole pH 7.0, temperature not specified in the publication 744675 1.14.14.3 1.79 - 4-N-(9-aldehyde-decyl)-7-N,N-dimethylsulfonic-2,1,3-benzoxadiazole pH 7.0, temperature not specified in the publication 744675