1.14.13.92 additional information - additional information detailed steady-state and pre-steady-state kinetic analysis of the reductive and the oxidative half-reaction of wild-type and mutant enzymes, overview 685231 1.14.13.92 additional information - additional information Michaelis-Menten kinetics 745991 1.14.13.92 additional information - additional information steady-state kinetic analysis of wild-type and mutant enzymes 765535 1.14.13.92 additional information - additional information steady-state kinetics 677171 1.14.13.92 additional information - additional information steady-state kinetics of wild-type and mutant enzymes 763862, 764409 1.14.13.92 additional information - additional information steady-state kinetics with NADPH and NADH cofactors, overview 710979 1.14.13.92 0.0006 - NADPH pH 8.0, 30°C, recombinant mutant H220N 710979 1.14.13.92 0.0007 - NADPH at pH 9.0 and 20°C 728164 1.14.13.92 0.0007 - NADPH pH 8.0, 30°C, recombinant mutant H220A 710979 1.14.13.92 0.0007 - NADPH pH 8.0, 30°C, recombinant wild-type PAMO 710979 1.14.13.92 0.0011 - NADPH pH 8.0, 30°C, recombinant mutant K336N 710979 1.14.13.92 0.0014 - NADPH pH 8.0, 30°C, recombinant mutant T218A 710979 1.14.13.92 0.0017 - NADPH pH 8.0, 30°C, recombinant mutant H220Q 710979 1.14.13.92 0.0017 - NADPH pH 8.0, 30°C, recombinant mutant H220T 710979 1.14.13.92 0.0023 - NADPH pH 8.0, 30°C, recombinant mutant H220W 710979 1.14.13.92 0.0024 - NADPH pH 8.0, 30°C, recombinant mutant H220Q/K336N 710979 1.14.13.92 0.011 - NADPH pH 8.0, 30°C, recombinant mutant H220Q/K336H 710979 1.14.13.92 0.011 - NADPH pH 8.0, 30°C, recombinant mutant K336H 710979 1.14.13.92 0.02 - NADPH pH 8.0, 30°C, recombinant mutant H220F 710979 1.14.13.92 0.036 - NADPH pH 8.0, 30°C, recombinant mutant H220D 710979 1.14.13.92 0.04 - phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C 726793 1.14.13.92 0.059 - phenylacetone 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 0.059 - phenylacetone wild-type enzyme 671189 1.14.13.92 0.06 - phenylacetone recombinant wild-type enzyme, pH 7.4, 25°C 765535 1.14.13.92 0.061 - phenylacetone 25°C, pH 8.5 659371 1.14.13.92 0.07 - 2-phenylcyclohexanone mutant P3 671189 1.14.13.92 0.08 - phenylacetone wild type enzyme, at pH 7.5 and 37°C 726793 1.14.13.92 0.1037 - cyclohexanone mutant P440F, pH 8.0, 45°C 764409 1.14.13.92 0.17 - NADPH pH 8.0, 30°C, recombinant mutant H220E 710979 1.14.13.92 0.17 - phenylacetone recombinant mutant R258A, pH 7.4, 25°C 765535 1.14.13.92 0.2 - 2-dodecanone 25°C, pH 8.5 659371 1.14.13.92 0.2107 - cyclohexanone mutant I67Y/P440F, pH 8.0, 45°C 764409 1.14.13.92 0.25 - 2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C 726793 1.14.13.92 0.25 - 2-Octanone recombinant mutant P253F/G254A/R258M/L443F, pH 7.4, 25°C 765535 1.14.13.92 0.2595 - cyclohexanone mutant I67G/P440F, pH 8.0, 45°C 764409 1.14.13.92 0.26 - 2-dodecanone 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 0.266 - cyclohexanone pH 8.0, 25°C, recombinant mutant S441G/A442P/L443T/S444Q 744548 1.14.13.92 0.28 - phenylacetone 25°C, pH 8.5, in the presence of 0.006 mM bovine serum albumin 659371 1.14.13.92 0.3 - 2-Octanone recombinant mutant R258M, pH 7.4, 25°C 765535 1.14.13.92 0.32 - NADPH pH 8.0, 30°C, recombinant mutant R217A 710979 1.14.13.92 0.34 - Ethionamide 25°C, pH 8.5 659371 1.14.13.92 0.3472 - cyclohexanone mutant I67A/P440F, pH 8.0, 45°C 764409 1.14.13.92 0.36 - benzylacetone 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 0.4412 - cyclohexanone mutant I67C/P440F, pH 8.0, 45°C 764409 1.14.13.92 0.5 - 2-phenylcyclohexanone mutant P2 671189 1.14.13.92 0.52 - benzylacetone 25°C, pH 8.5 659371 1.14.13.92 0.698 - cyclohexanone pH 8.0, 25°C, recombinant mutant A442P/L443V 744548 1.14.13.92 0.8 - 2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C 726793 1.14.13.92 0.8 - 2-phenylcyclohexanone recombinant mutant P253F/G254A/R258M/L443F, pH 7.4, 25°C 765535 1.14.13.92 0.83 - (2-methylphenyl)acetone 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 0.85 - NADPH pH 8.0, 30°C, recombinant mutant R217L 710979 1.14.13.92 0.86 - methyl 4-tolylsulfide 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 1.4 - phenylacetone recombinant mutant P253F/G254A/R258M/L443F, pH 7.4, 25°C 765535 1.14.13.92 2 - 2-Octanone wild type enzyme, at pH 7.5 and 37°C 726793 1.14.13.92 2.1 - 2-Octanone recombinant mutant R258A, pH 7.4, 25°C 765535 1.14.13.92 2.2 - 4-hydroxyacetophenone 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 2.3 - 2-phenylcyclohexanone mutant P1 671189 1.14.13.92 2.5 - phenylacetone mutant P3 671189 1.14.13.92 3 - phenylacetone mutant P1 671189 1.14.13.92 3.2 - 2-Octanone recombinant wild-type enzyme, pH 7.4, 25°C 765535 1.14.13.92 4 - 2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C 726793 1.14.13.92 4 - phenylacetone mutant P2 671189 1.14.13.92 15 - bicyclohept-2-en-6-one 30°C, pH 7.5, 0.1 mM NADPH 657625 1.14.13.92 44 - 2-phenylcyclohexanone recombinant wild-type enzyme, pH 7.4, 25°C 765535 1.14.13.92 1000 - Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C 726793 1.14.13.92 1000 - Cyclopentanone recombinant mutant P253F/G254A/R258M/L443F, pH 7.4, 25°C 765535 1.14.13.92 1200 - Cyclopentanone recombinant wild-type enzyme, pH 7.4, 25°C 765535 1.14.13.92 1200 - Cyclopentanone wild type enzyme, at pH 7.5 and 37°C 726793