1.13.12.5 additional information - additional information effects of trehalose and sucrose as chemical chaperones on the kinetic stability of Rluc, activity of the enzyme in presence of these additives at high temperatures, mechanism of stability, molecular dynamic simulation, modeliing, overview 745101 1.13.12.5 additional information - additional information enzyme kinetics in presence and absence of imidazolium-based ionic liquids, overview 744253 1.13.12.5 additional information - additional information enzyme kinetics in presence of inhibitors DMSO and isopropanol, overview 745096 1.13.12.5 additional information - additional information Michaelis-Menten kinetics 744017, 745957 1.13.12.5 0.0016 - coelenterazine mutant enzyme A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L 676939 1.13.12.5 0.00214 - coelenterazine K0.5 value, Hill coefficient 1.8, pH 7.5, temperature not specified in the publication 765264 1.13.12.5 0.0025 - coelenterazine wild-type enzyme, pH 7.2, 25°C 744017 1.13.12.5 0.0029 - coelenterazine wild-type enzyme 676939 1.13.12.5 0.00293 - coelenterazine pH 7.2, 37°C, recombinant engineered mutant super RLuc 8 744813 1.13.12.5 0.003 - coelenterazine modified enzyme FcUni-RLuc, pH 7.2, 25°C 744017 1.13.12.5 0.0031 - coelenterazine pH 7.8, 25°C, recombinant His-tagged wild-type enzyme 744253 1.13.12.5 0.0039 - coelenterazine h pH 7.8, 25°C, recombinant His-tagged enzyme 745096 1.13.12.5 0.0068 0.0103 coelenterazine pH 7.8, 25°C, recombinant His-tagged wild-type enzyme in presence of 13.3-26.7 mM 1-butyl-3-methylimidazolium tetrafluoroborate 744253 1.13.12.5 0.00728 - coelenterazine pH 7.2, 37°C, recombinant modified enzyme C-SRLuc8 744428 1.13.12.5 0.0097 0.0119 coelenterazine pH 7.8, 25°C, recombinant His-tagged wild-type enzyme in presence of 4.8-9.7 mM 1-butyl-3-methylimidazoliumhexafluorophosphate 744253 1.13.12.5 0.00997 - coelenterazine pH 7.2, 37°C, recombinant wild-type enzyme 744813 1.13.12.5 0.00997 - coelenterazine pH 7.2, 37°C, recombinant wild-type enzyme SRLuc8 744428 1.13.12.5 0.0133 - coelenterazine reaction with BFP-aq 673608