1.13.11.50 additional information - additional information kinetic first-order rate constants of substrate ligation and steady-state kinetic studies of wild-type and mutant enzymes, overview 711267 1.13.11.50 additional information - additional information steady-state and single-turnover kinetics of substrate binding and conversion in wild-type Dke1 and mutants, overview 725553 1.13.11.50 0.000009 - Pentane-2,4-dione - 636327 1.13.11.50 0.002 - 4,4-difluoro-1-phenyl-1,3-butanedione apparent value at 25°C 674150 1.13.11.50 0.0025 - 1,1,1-trifluoro-2,4-pentanedione apparent value at 25°C 674150 1.13.11.50 0.0027 - 1,1-difluoro-2,4-pentanedione apparent value at 25°C 674150 1.13.11.50 0.0034 - Pentane-2,4-dione pH 7.5, 25°C 657614 1.13.11.50 0.006 - Pentane-2,4-dione Strep-tagged enzyme, at pH 7.5 671683 1.13.11.50 0.006 - Pentane-2,4-dione wild type enzyme, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C 675466 1.13.11.50 0.0065 - Pentane-2,4-dione mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C 675466 1.13.11.50 0.008 - Pentane-2,4-dione recombinant enzyme, at pH 7.5 671683 1.13.11.50 0.009 - Pentane-2,4-dione native enzyme, at pH 7.5 671683 1.13.11.50 0.0091 - acetylacetate - 657866 1.13.11.50 0.0091 - Pentane-2,4-dione apparent value at 25°C 674150 1.13.11.50 0.26 - O2 pH 7.5, 25°C 657614