1.1.1.345 additional information - additional information Michaelis-Menten kinetics, recombinant enzyme 743180 1.1.1.345 0.01 - NADH pH 7.0, 25°C 731190 1.1.1.345 0.02 - 2-Oxohexanoate pH 7.5, 25°C 731741 1.1.1.345 0.022 - 4-methyl-2-oxopentanoate pH 7.5, 25°C 731741 1.1.1.345 0.031 - phenylpyruvate pH 7.5, 25°C 731741 1.1.1.345 0.05 - NADH with 2-oxobutyrate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C 672434 1.1.1.345 0.057 - 2-oxopentanoate pH 7.5, 25°C 731741 1.1.1.345 0.0591 - phenylpyruvate recombinant His-tagged enzyme, pH and temperature not specified in the publication 743180 1.1.1.345 0.06 - 4-methyl-2-oxopentanoate pH 7.0, 25°C 731190 1.1.1.345 0.086 - NADH with 2-oxoisocaproate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C 672434 1.1.1.345 0.096 - NADH with pyruvate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C 672434 1.1.1.345 0.11 - 2-Oxohexanoate pH 7.0, 25°C 731190 1.1.1.345 0.11 - 2-oxopentanoate pH 7.0, 25°C 731190 1.1.1.345 0.11 - 4-methyl-2-oxopentanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 0.12 - 2-oxooctanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 0.12 - 2-oxopentanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 0.13 - 2-Oxohexanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 0.13 - 4-methyl-2-oxopentanoate pH 7.5, 30°C 685675 1.1.1.345 0.14 - D-lactate in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C 672434 1.1.1.345 0.14 - NADH at pH 7.0 and 37°C 712292 1.1.1.345 0.15 - 3-methyl-2-oxobutanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 0.15 - phenylpyruvate pH 7.0, 25°C 731190 1.1.1.345 0.18 - 3-methyl-2-oxobutanoate pH 7.5, 30°C 685675 1.1.1.345 0.2 - phenylpyruvate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 0.21 - 2-oxomethylvalerate at pH 7.0 and 37°C 712292 1.1.1.345 0.21 - 3-methyl-2-oxopentanoate pH 7.0, 37°C 712292 1.1.1.345 0.25 - phenylglyoxylate pH 7.5, 30°C 685675 1.1.1.345 0.26 - phenylglyoxylate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 0.29 - 2-oxoisovalerate at pH 7.0 and 37°C 712292 1.1.1.345 0.29 - 3-methyl-2-oxobutanoate pH 7.0, 37°C 712292 1.1.1.345 0.3 - 2-oxoisocaproate at pH 7.0 and 37°C 712292 1.1.1.345 0.3 - 2-oxopentanoate pH 7.5, 30°C 685675 1.1.1.345 0.3 - 4-methyl-2-oxopentanoate pH 7.0, 37°C 712292 1.1.1.345 0.36 - 2-oxobutyrate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C 672434 1.1.1.345 0.4 - 2-oxopentanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 0.4 - 4-methyl-2-oxopentanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 0.5 - NAD+ pH 7.0, 25°C 731190 1.1.1.345 0.53 - 2-oxovalerate at pH 7.0 and 37°C 712292 1.1.1.345 0.56 - pyruvate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C 672434 1.1.1.345 0.6 - 2-Oxohexanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 0.6 - 3-(4-hydroxyphenyl)-2-oxopropanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 0.76 - (R)-mandelate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 0.9 - (R)-mandelate pH 7.5, 30°C 685675 1.1.1.345 1.2 - pyruvate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 1.26 - 2-formylbutanethioate pH 7.0, 37°C 712292 1.1.1.345 1.26 - 4-methylthio-2-oxobutanoate at pH 7.0 and 37°C 712292 1.1.1.345 1.5 - 2-Oxohexanoate pH 7.5, 30°C 685675 1.1.1.345 1.5 - benzoylformate at pH 7.0 and 37°C 712292 1.1.1.345 1.5 - benzoylformate pH 7.0, 37°C 712292 1.1.1.345 1.6 - 2-Hydroxyoctanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 2.3 - (R)-2-hydroxy-4-methylpentanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 2.3 - 2-oxoisocaproate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C 672434 1.1.1.345 2.4 - 2-oxocaproate at pH 7.0 and 37°C 712292 1.1.1.345 2.7 - 2-hydroxyhexanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 2.9 - 3-hydroxypyruvate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 3 - D-mandelate at pH 7.0 and 37°C 712292 1.1.1.345 3 - DL-2-hydroxyisocaproate at pH 7.0 and 37°C 712292 1.1.1.345 3.3 - 2-hydroxypentanoate pH 7.0, temperature not specified in the publication 731727 1.1.1.345 3.4 - 2-oxo-3-phenylpropanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 4 - 2-oxobutanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 729022 1.1.1.345 5.4 - phenylpyruvate at pH 7.0 and 37°C 712292 1.1.1.345 5.4 - phenylpyruvate pH 7.0, 37°C 712292 1.1.1.345 5.7 - 2-oxo-3-phenylpropanoate pH 7.5, 30°C 685675 1.1.1.345 9.5 - 2-oxobutanoate pH 7.5, 30°C 685675 1.1.1.345 20 - phenylpyruvate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 28 - 2-oxobutyrate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 40 - 2-oxocaproate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 46 - 2-oxoisovalerate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 62 - 2-oxovalerate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588 1.1.1.345 71 - 2-oxoisocaproate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C 722588