1.1.1.3 additional information - additional information enzyme HseDH shows typical Michaelis-Menten kinetics for oxidation 741408 1.1.1.3 additional information - additional information kinetic profile 739791 1.1.1.3 additional information - additional information kinetics 642340 1.1.1.3 additional information - additional information Michaelis-Menten steady-state kinetics 761404 1.1.1.3 additional information - additional information the binding of L-homoserine is the rate limiting factor for L-homoserine oxidation, Michaelis-Menten kinetics, overview 761687 1.1.1.3 0.013 - L-homoserine - 246387 1.1.1.3 0.027 - NADPH isozyme II 246382 1.1.1.3 0.028 - NADPH pH 8.0, 25°C 712490 1.1.1.3 0.031 - NADPH threonine sensitive isozyme 246388 1.1.1.3 0.032 0.036 NADPH threonine resistant isozyme 246388 1.1.1.3 0.034 - NADP+ pH 8.0, 25°C 712490 1.1.1.3 0.036 - NADPH threonine resistant isozyme 246385 1.1.1.3 0.036 - NADPH threonine-resistant enzyme, pH and temperature not specified in the publication 246385 1.1.1.3 0.039 - NADP+ pH 9.0, 25°C, recombinant enzyme 761687 1.1.1.3 0.039 - NADPH pH 8.0, 25°C 712490 1.1.1.3 0.04 - aspartate 4-semialdehyde isozyme II 246382 1.1.1.3 0.04 - NADP+ pH 9.0, 50°C, recombinant mutant R40A 741408 1.1.1.3 0.04 - NADPH threonine sensitive isozyme 246385 1.1.1.3 0.04 - NADPH threonine-sensitive enzyme, pH and temperature not specified in the publication 246385 1.1.1.3 0.043 - NADPH threonine sensitive isozyme 246388 1.1.1.3 0.046 - NADPH isozyme I 246382 1.1.1.3 0.05 - NAD+ pH 9.0, 50°C, recombinant mutant K57A 741408 1.1.1.3 0.06 - NADP+ pH 9.0, 50°C, recombinant mutant K57A 741408 1.1.1.3 0.064 - NADP+ recombinant enzyme, pH 10.5, 55°C 760736 1.1.1.3 0.066 - L-aspartate 4-semialdehyde isozyme I 246382 1.1.1.3 0.073 - NADP+ - 246387 1.1.1.3 0.08 - L-aspartate 4-semialdehyde threonine insensitive isozyme 246391 1.1.1.3 0.09 - NADPH - 246387 1.1.1.3 0.098 - L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C 712490 1.1.1.3 0.1 - DL-aspartate 4-semialdehyde - 246395 1.1.1.3 0.1 - L-aspartate 4-semialdehyde isozyme I 246382 1.1.1.3 0.11 - NADP+ pH 10.0, 50°C, recombinant enzyme 761404 1.1.1.3 0.13 0.15 L-aspartate 4-semialdehyde threonine resistant isozyme 246388 1.1.1.3 0.15 - L-aspartate 4-semialdehyde threonine resistant isozyme 246385 1.1.1.3 0.15 - L-aspartate 4-semialdehyde threonine-resistant enzyme, pH and temperature not specified in the publication 246385 1.1.1.3 0.158 - NADH pH 8.0, 25°C 712490 1.1.1.3 0.166 - NADP+ pH 8.0, 37°C, purified recombinant soluble enzyme 642340 1.1.1.3 0.17 - L-aspartate 4-semialdehyde - 246387 1.1.1.3 0.17 0.18 NADP+ - 246392, 246393 1.1.1.3 0.19 - NADH pH 8.0, 25°C 712490 1.1.1.3 0.2 - L-homoserine pH 10.0, 50°C, recombinant enzyme, with NADP+ 761404 1.1.1.3 0.21 - L-homoserine pH 8.0, 30°C, oxidized enzyme 739972 1.1.1.3 0.213 - NADH pH 8.0, 25°C 712490 1.1.1.3 0.235 - NADP+ pH 8.0, 25°C 712490 1.1.1.3 0.24 0.25 L-aspartate 4-semialdehyde threonine sensitive isozyme 246388 1.1.1.3 0.245 - NADP+ pH 8.0, 25°C 712490 1.1.1.3 0.25 - L-aspartate 4-semialdehyde threonine sensitive isozyme 246385 1.1.1.3 0.25 - L-aspartate 4-semialdehyde threonine-sensitive enzyme, pH and temperature not specified in the publication 246385 1.1.1.3 0.275 - L-homoserine cosubstrate NADP+, pH 8.0, 25°C 712490 1.1.1.3 0.3 - NADH - 246395 1.1.1.3 0.31 - NAD+ pH 8.0, 30°C, oxidized enzyme 739972 1.1.1.3 0.32 - NAD+ pH 9.0, 50°C, recombinant wild-type enzyme 741408 1.1.1.3 0.33 - NAD+ pH 8.0, 30°C 762453 1.1.1.3 0.33 - NAD+ pH 8.0, 30°C, reduced enzyme 739972 1.1.1.3 0.36 0.4 L-aspartate 4-semialdehyde isozyme II 246382 1.1.1.3 0.41 - L-homoserine recombinant hybrid bifunctional holoenzyme AKIII-HDHI+ containing the interface region, homoserine dehydrogenase activity 654640 1.1.1.3 0.46 - NADH isoenzyme II, at pH 7.5, temperature not specified in the publication 246382 1.1.1.3 0.46 - NADH isozyme II 246382 1.1.1.3 0.5 - L-aspartate 4-semialdehyde - 246395 1.1.1.3 0.5 - L-aspartate 4-semialdehyde threonine sensitive isozyme 246391 1.1.1.3 0.54 - L-homoserine pH 8.0, 30°C, reduced enzyme 739972 1.1.1.3 0.569 - L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C 712490 1.1.1.3 0.68 - L-homoserine recombinant isolated catalytic HDH-domain containing the interface region, homoserine dehydrogenase activity 654640 1.1.1.3 0.69 - L-homoserine cosubstrate NADP+, pH 8.0, 25°C 712490 1.1.1.3 0.74 - NAD+ pH 9.5, 50°C, recombinant enzyme 761404 1.1.1.3 0.845 - L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C 712490 1.1.1.3 0.95 - NAD+ pH 9.0, 50°C, recombinant mutant R40A 741408 1.1.1.3 1.08 - L-homoserine cosubstrate NADP+, pH 8.0, 25°C 712490 1.1.1.3 1.08 - L-homoserine pH 9.5, 50°C, recombinant enzyme, with NAD+ 761404 1.1.1.3 1.19 - L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C 712490 1.1.1.3 1.2 - L-homoserine recombinant wild-type bifunctional holoenzyme, homoserine dehydrogenase activity 654640 1.1.1.3 1.2 - NADP+ pH 8.0, 30°C 762453 1.1.1.3 1.25 - L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C 712490 1.1.1.3 1.81 - L-homoserine recombinant enzyme, pH 10.5, 55°C 760736 1.1.1.3 2.19 - L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C 712490 1.1.1.3 2.7 - NAD+ pH 8.0, 25°C 712490 1.1.1.3 5.2 - L-homoserine pH 8.0, 37°C, purified recombinant soluble enzyme 642340 1.1.1.3 5.5 - ATP pH 8.0, 37°C, purified recombinant soluble enzyme 642340 1.1.1.3 6.1 - L-homoserine pH 9.0, 50°C, recombinant enzyme 741408 1.1.1.3 9.57 - L-homoserine cosubstrate NAD+, pH 8.0, 25°C 712490 1.1.1.3 11.6 - L-aspartate pH 8.0, 37°C, purified recombinant soluble enzyme 642340 1.1.1.3 13.4 - L-homoserine cosubstrate NAD+, pH 8.0, 25°C 712490 1.1.1.3 17.2 - L-homoserine recombinant isolated catalytic HDH-domain not containing the interface region, homoserine dehydrogenase activity 654640 1.1.1.3 17.4 - L-homoserine cosubstrate NAD+, pH 8.0, 25°C 712490 1.1.1.3 24.1 - NAD+ pH 8.0, 25°C 712490 1.1.1.3 24.9 - NAD+ pH 8.0, 25°C 712490 1.1.1.3 35.08 - L-homoserine pH 9.0, 25°C, recombinant enzyme 761687