1.4.1.3 0.000175 - NADH - 391594 1.4.1.3 0.00294 - alpha-ketoglutarate - 391594 1.4.1.3 0.0424 - NH4+ - 391594 1.4.1.3 3.2 - NH4+ - 391602 1.4.1.3 0.11 - NADH - 391604 1.4.1.3 0.13 - NADPH - 391604 1.4.1.3 0.46 - NAD+ - 391604 1.4.1.3 2.1 - 2-oxoglutarate cosubstrate NADPH 391604 1.4.1.3 3.3 - 2-oxoglutarate cosubstrate NADH 391604 1.4.1.3 12 - glutamate cosubstrate NAD+ 391604 1.4.1.3 15 - NH4+ cosubstrates NADPH + 2-oxoglutarate 391604 1.4.1.3 27 - NH4+ cosubstrates NADH + 2-oxoglutarate 391604 1.4.1.3 0.009 - NADH glutamate dehydrogenase 1, 5 mM 2-oxoglutarate 391607 1.4.1.3 0.009 - NADH glutamate dehydrogenase 2, 5 mM 2-oxoglutarate 391607 1.4.1.3 0.012 - NADH glutamate dehydrogenase 2, 80 mM NH4+ 391607 1.4.1.3 0.02 - NADH glutamate dehydrogenase 1, 40 mM NH4+ 391607 1.4.1.3 0.028 - NADPH glutamate dehydrogenase 1, 10 mM 2-oxoglutarate 391607 1.4.1.3 0.028 - NADPH glutamate dehydrogenase 2, 10 mM 2-oxoglutarate 391607 1.4.1.3 0.048 - NADH glutamate dehydrogenase 1, 400 mM NH4+ 391607 1.4.1.3 0.05 - NADH glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate 391607 1.4.1.3 0.069 - NADPH glutamate dehydrogenase 1, 40 mM NH4+ 391607 1.4.1.3 0.069 - NADPH glutamate dehydrogenase 2, 80 mM NH4+ 391607 1.4.1.3 0.62 - 2-oxoglutarate glutamate dehydrogenase 2, 0.1 mM NADH 391607 1.4.1.3 1.1 - 2-oxoglutarate glutamate dehydrogenase 2, 80 mM NH4+ 391607 1.4.1.3 1.51 - 2-oxoglutarate glutamate dehydrogenase 1, 0.1 mM NADH 391607 1.4.1.3 1.51 - 2-oxoglutarate glutamate dehydrogenase 1, 400 mM NH4+ 391607 1.4.1.3 2.1 - 2-oxoglutarate glutamate dehydrogenase 1, 40 mM NH4+ 391607 1.4.1.3 2.6 - 2-oxoglutarate glutamate dehydrogenase 1, 0.2 mM NADPH 391607 1.4.1.3 3.7 - 2-oxoglutarate glutamate dehydrogenase 1, 0.2 mM NADH 391607 1.4.1.3 3.7 - 2-oxoglutarate glutamate dehydrogenase 2, 0.2 mM NADPH 391607 1.4.1.3 5.8 - NH4+ glutamate dehydrogenase 1, 0.2 mM NADPH 391607 1.4.1.3 7 - NH4+ glutamate dehydrogenase 1, 0.2 mM NADPH 391607 1.4.1.3 10.4 - NH4+ glutamate dehydrogenase 1, 10 mM 2-oxoglutarate 391607 1.4.1.3 12.9 - NH4+ glutamate dehydrogenase 2, 5 mM 2-oxoglutarate 391607 1.4.1.3 15.8 - NH4+ glutamate dehydrogenase 1, 5 mM 2-oxoglutarate 391607 1.4.1.3 19 - NH4+ glutamate dehydrogenase 1, 10 mM 2-oxoglutarate 391607 1.4.1.3 21.7 - NH4+ glutamate dehydrogenase 1, 0.1 mM NADH 391607 1.4.1.3 22.8 - NH4+ glutamate dehydrogenase 1, 0.1 mM NADH 391607 1.4.1.3 106 - NH4+ glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate 391607 1.4.1.3 115.1 - NH4+ glutamate dehydrogenase 1, 0.2 mM NADH 391607 1.4.1.3 0.004 - NADH 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.01 - NADH 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 0.012 - NADH 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.016 - NAD+ 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.11 - NAD+ 500 mM Tris-HCl, pH 9.4 391609 1.4.1.3 0.15 - NAD+ 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.17 - NADPH 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.4 - 2-oxoglutarate 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.54 - NADPH 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.75 - NADPH 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 1.2 - 2-oxoglutarate 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 1.7 - L-glutamate 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 14 - 2-oxoglutarate 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 19.5 - L-glutamate 500 mM Tris-HCl, pH 9.4 391609 1.4.1.3 31 - L-glutamate 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 58 - NH4+ 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 77 - NH4+ 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 160 - NH4+ 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.022 - NADPH - 391612 1.4.1.3 0.028 - NADP+ cosubstrate glutamate 391612 1.4.1.3 0.31 - NADP+ cosubstrate norvaline 391612 1.4.1.3 0.74 - L-glutamate - 391612 1.4.1.3 2.4 - 2-oxoglutarate - 391612 1.4.1.3 6.5 - NH4+ - 391612 1.4.1.3 49 - norvaline - 391612 1.4.1.3 0.02 - NADH - 391614 1.4.1.3 0.02 - NADPH - 391614 1.4.1.3 0.36 - 2-oxoglutarate NADH + NH4Cl 391614 1.4.1.3 0.47 - 2-oxoglutarate NADPH + NH4Cl 391614 1.4.1.3 1 - L-glutamate 1 mM, NAD+ 391614 1.4.1.3 3 - L-glutamate 1 mM, NADP+ 391614 1.4.1.3 25 - L-glutamate 0.004 mM, NAD+ 391614 1.4.1.3 44 - NH3 NADPH + 2-oxoglutarate 391614 1.4.1.3 50 - NH3 NADH + 2-oxoglutarate 391614 1.4.1.3 0.004 - NADP+ NADP+-dependent activity 391615 1.4.1.3 0.013 - NADPH NADPH-dependent activity 391615 1.4.1.3 0.25 - 2-oxoglutarate NADPH-dependent activity 391615 1.4.1.3 0.38 - NH3 biphasic Lineweaver-Burk plot suggests 2 Km values 391615 1.4.1.3 0.5 - NADH NADH-dependent activity 391615 1.4.1.3 5 - 2-oxoglutarate NADH-dependent activity 391615 1.4.1.3 10 - L-glutamate NADP+-dependent activity 391615 1.4.1.3 66 - NH3 NADH-dependent activity 391615 1.4.1.3 100 - NH3 biphasic Lineweaver-Burk plot suggests 2 Km values 391615 1.4.1.3 0.052 - N6-(3-sulfonatopropyl)-NAD+ - 391617 1.4.1.3 0.148 - N6-(2-hydroxy-3-trimethylammoniumpropyl)-NAD+ - 391617 1.4.1.3 0.16 - NADP+ - 391617 1.4.1.3 0.175 - NAD+ - 391617 1.4.1.3 0.273 - N6-(2-aminoethyl)-NADP+ - 391617 1.4.1.3 0.291 - N6-(2-aminoethyl)-NAD+ - 391617 1.4.1.3 0.425 - poly(ethyleneglycol)-N6-(2-aminoethyl)-NADP+ - 391617 1.4.1.3 0.444 - poly(ethyleneglycol)-N6-(2-aminoethyl)-NAD+ - 391617 1.4.1.3 0.013 - NADPH NADP-linked reductive amination 391618 1.4.1.3 0.019 - NADP+ NADP-linked oxidative deamination 391618 1.4.1.3 0.14 - 2-oxoglutarate NADP-linked reductive amination 391618 1.4.1.3 0.2 - NADH NAD-linked reductive amination 391618 1.4.1.3 1.7 - NH3 NADP-linked reductive amination 391618 1.4.1.3 2.4 - L-glutamate NADP-linked oxidative deamination 391618 1.4.1.3 3 - NAD+ NAD-linked oxidative deamination 391618 1.4.1.3 4.9 - NH3 NAD-linked reductive amination 391618 1.4.1.3 5.1 - NH3 NADP-linked reductive amination 391618 1.4.1.3 7.1 - 2-oxoglutarate NAD-linked reductive amination 391618 1.4.1.3 7.3 - L-glutamate NAD-linked oxidative deamination 391618 1.4.1.3 0.006 - NADPH NADPH-dependent amination 391619 1.4.1.3 0.018 - NADPH NADPH-dependent amination 391619 1.4.1.3 0.02 - NADPH NADPH-dependent amination 391619 1.4.1.3 0.63 - 2-oxoglutarate NADPH-dependent amination 391619 1.4.1.3 0.64 - 2-oxoglutarate NADPH-dependent amination 391619 1.4.1.3 0.7 - 2-oxoglutarate NADPH-dependent amination 391619 1.4.1.3 1.5 - NH3 NADPH-dependent amination 391619 1.4.1.3 2.4 - NH3 NADPH-dependent amination 391619 1.4.1.3 2.9 - NH3 NADPH-dependent amination 391619 1.4.1.3 0.011 - NADPH reductive amination, low-activity form of the enzyme 391620 1.4.1.3 0.013 - NADP+ oxidative deamination, low-activity form of the enzyme 391620 1.4.1.3 0.013 - NADPH reductive amination, high-activity form of the enzyme 391620 1.4.1.3 0.029 - NADP+ oxidative deamination, high-activity form of the enzyme 391620 1.4.1.3 0.13 - NADH reductive amination, low-activity form of the enzyme 391620 1.4.1.3 0.18 - 2-oxoglutarate reductive amination, low-activity form of the enzyme 391620 1.4.1.3 0.2 - 2-oxoglutarate reductive amination, high-activity form of the enzyme 391620 1.4.1.3 0.43 - NAD+ oxidative deamination, low-activity form of the enzyme 391620 1.4.1.3 0.52 - NADH reductive amination, high-activity form of the enzyme 391620 1.4.1.3 1.25 - NH3 reductive amination, high-activity form of the enzyme 391620 1.4.1.3 1.31 - NH3 reductive amination, low-activity form of the enzyme 391620 1.4.1.3 2.44 - NAD+ oxidative deamination, high-activity form of the enzyme 391620 1.4.1.3 3.65 - L-glutamate oxidative deamination, low-activity form of the enzyme 391620 1.4.1.3 3.83 - L-glutamate oxidative deamination, high-activity form of the enzyme 391620 1.4.1.3 0.007 - NADH + 2-oxoglutarate 391621 1.4.1.3 0.01 - NADPH + 2-oxoglutarate 391621 1.4.1.3 0.025 - NADP+ + L-glutamate 391621 1.4.1.3 0.063 - NAD+ + L-glutamate 391621 1.4.1.3 0.2 - 2-oxoglutarate cosubstrate NADPH 391621 1.4.1.3 0.3 - L-glutamate cosubstrate NAD+ 391621 1.4.1.3 0.6 - 2-oxoglutarate cosubstrate NADH 391621 1.4.1.3 1.1 - L-glutamate cosubstrate NADP+ 391621 1.4.1.3 0.07 - NADH isozyme 1, amination activity, cofactor NADH 391622 1.4.1.3 0.09 - NADH isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 0.14 - NADH isozyme 2, amination activity, cofactor NADH 391622 1.4.1.3 0.2 - NAD+ isozyme 1, deamination activity 391622 1.4.1.3 0.26 - NAD+ isozyme 2, deamination activity 391622 1.4.1.3 0.27 - NADPH isozyme 1, amination activity, cofactor NADPH 391622 1.4.1.3 0.32 - NAD+ isozyme 3, deamination activity 391622 1.4.1.3 0.36 - 2-oxoglutarate isozyme 1, amination activity, cofactor NADH 391622 1.4.1.3 0.46 - NADPH isozyme 3, amination activity, cofactor NADPH 391622 1.4.1.3 0.59 - 2-oxoglutarate isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 0.64 - L-glutamate isozyme 1, deamination activity 391622 1.4.1.3 0.78 - NADPH isozyme 2, amination activity, cofactor NADPH 391622 1.4.1.3 1.23 - 2-oxoglutarate isozyme 2, amination activity, cofactor NADH 391622 1.4.1.3 1.54 - 2-oxoglutarate isozyme 1, amination activity, cofactor NADPH 391622 1.4.1.3 1.73 - 2-oxoglutarate isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 1.85 - 2-oxoglutarate isozyme 2, amination activity, cofactor NADPH 391622 1.4.1.3 3 - L-glutamate isozyme 3, deamination activity 391622 1.4.1.3 3.52 - L-glutamate isozyme 2, deamination activity 391622 1.4.1.3 13 - NH4+ isozyme 2, amination activity, cofactor NADH 391622 1.4.1.3 30 - NH4+ isozyme 1, amination activity, cofactor NADH 391622 1.4.1.3 36 - NH4+ isozyme 1, amination activity, cofactor NADPH 391622 1.4.1.3 41 - NH4+ isozyme 3, amination activity, cofactor NADPH 391622 1.4.1.3 44 - NH4+ isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 53 - NH4+ isozyme 2, amination activity, cofactor NADPH 391622 1.4.1.3 0.18 - NADP+ substrate L-glutamate 391623 1.4.1.3 0.53 - NAD+ substrate L-glutamate 391623 1.4.1.3 0.56 - NADPH substrate 2-oxoglutarate 391623 1.4.1.3 0.56 - NADPH substrate NH3 391623 1.4.1.3 0.98 - NADH substrate 2-oxoglutarate 391623 1.4.1.3 1.6 - L-glutamate cofactor NADP+ 391623 1.4.1.3 1.66 - L-glutamate cofactor NAD+ 391623 1.4.1.3 4 - 2-oxoglutarate cofactor NADPH 391623 1.4.1.3 4.5 - 2-oxoglutarate cofactor NADH 391623 1.4.1.3 9.5 - NH3 cofactor NADPH 391623 1.4.1.3 0.015 - NADH liver enzyme 391624 1.4.1.3 0.022 - NADH kidney enzyme 391624 1.4.1.3 0.032 - NAD+ kidney enzyme 391624 1.4.1.3 0.4 - L-glutamate kidney enzyme 391624 1.4.1.3 1 - 2-oxoglutarate liver enzyme 391624 1.4.1.3 2.3 - 2-oxoglutarate kidney enzyme 391624 1.4.1.3 20 - NH3 kidney enzyme 391624 1.4.1.3 21 - NH3 liver enzyme 391624 1.4.1.3 0.0083 - NADH - 391631 1.4.1.3 0.011 - NADPH - 391631 1.4.1.3 0.014 - NAD+ - 391631 1.4.1.3 0.7 - 2-oxoglutarate cofactor NADH 391631 1.4.1.3 2.1 - glutamate - 391631 1.4.1.3 6.3 - 2-oxoglutarate cofactor NADPH 391631 1.4.1.3 28 - NH3 cofactor NADH 391631 1.4.1.3 41 - NH3 cofactor NADPH 391631 1.4.1.3 0.04 - NADPH concentration range: 0.002-0.1 mM 391632 1.4.1.3 0.08 - NADP+ concentration range: 0.02-0.2 mM 391632 1.4.1.3 0.09 - NADH concentration range: 0.04-0.1 mM 391632 1.4.1.3 0.25 - 2-oxoglutarate concentration range: 0.3-4.0 mM 391632 1.4.1.3 0.32 - glutamate concentration range: 0.05-1.0 mM 391632 1.4.1.3 1.3 - NH4+ concentration range: 0.2-5 mM 391632 1.4.1.3 5.5 - glutamate concentration range: 1.0-10.0 mM 391632 1.4.1.3 13 - NH4+ concentration range: 10-200 mM 391632 1.4.1.3 0.364 - NAD+ mitochondrial enzyme 391634 1.4.1.3 0.443 - NADP+ enzyme from rough ER 391634 1.4.1.3 0.637 - NADP+ mitochondrial enzyme 391634 1.4.1.3 0.924 - NAD+ enzyme from rough ER 391634 1.4.1.3 4.61 - glutamate mitochondrial enzyme, cofactor NAD+ 391634 1.4.1.3 5.93 - glutamate enzyme from rough ER, cofactor NAD+ 391634 1.4.1.3 20.7 - glutamate mitochondrial enzyme, cofactor NADP+ 391634 1.4.1.3 23.8 - glutamate enzyme from rough ER, cofactor NADP+ 391634 1.4.1.3 0.1 - 2-oxoglutarate euthermic animal, assay at 37°C 391636 1.4.1.3 0.25 - glutamate hibernating animal, assay at 5°C 391636 1.4.1.3 0.43 - 2-oxoglutarate hibernating animal, assay at 5°C 391636 1.4.1.3 0.47 - glutamate assay at 5°C 391636 1.4.1.3 0.5 - glutamate euthermic animal, assay at 5°C 391636 1.4.1.3 0.98 - 2-oxoglutarate hibernating animal, assay at 37°C 391636 1.4.1.3 1.4 - 2-oxoglutarate - 391636 1.4.1.3 1.4 - 2-oxoglutarate assay at 37°C 391636 1.4.1.3 2.03 - glutamate euthermic animal, assay at 37°C 391636 1.4.1.3 2.6 - glutamate assay at 37°C 391636 1.4.1.3 3.66 - 2-oxoglutarate euthermic animal, assay at 5°C 391636 1.4.1.3 4.1 - NH4+ - 391636 1.4.1.3 4.1 - NH4+ assay at 5°C 391636 1.4.1.3 5.2 - glutamate hibernating animal, assay at 37°C 391636 1.4.1.3 7 - NH4+ euthermic animal, assay at 37°C 391636 1.4.1.3 10.5 - 2-oxoglutarate - 391636 1.4.1.3 10.5 - 2-oxoglutarate assay at 5°C 391636 1.4.1.3 12.1 - NH4+ hibernating animal, assay at 5°C 391636 1.4.1.3 15.8 - NH4+ hibernating animal, assay at 37°C 391636 1.4.1.3 17.8 - NH4+ - 391636 1.4.1.3 17.8 - NH4+ assay at 37°C 391636 1.4.1.3 24.7 - NH4+ euthermic animal, assay at 5°C 391636 1.4.1.3 0.025 - NADP+ pH 7.6, 90°C 654356 1.4.1.3 0.037 - NADPH pH 7.6, 90°C 654356 1.4.1.3 0.083 - NADH pH 7.6, 90°C 654356 1.4.1.3 0.53 - 2-oxoglutarate pH 7.6, 90°C 654356 1.4.1.3 0.6 - L-glutamate pH 7.6, 90°C 654356 1.4.1.3 2.82 - NH4+ pH 7.6, 90°C 654356 1.4.1.3 0.08 - NADH pH 8.0, 25°C, wild-type enzyme 654650 1.4.1.3 0.082 - NADH pH 8.0, 25°C, mutant enzyme Y187E 654650 1.4.1.3 0.082 - NADH pH 8.0, 25°C, mutant enzyme Y187R 654650 1.4.1.3 0.084 - NADH pH 8.0, 25°C, mutant enzyme Y187M 654650 1.4.1.3 0.085 - NADH pH 8.0, 25°C, mutant enzyme Y187G 654650 1.4.1.3 0.088 - NADH pH 8.0, 25°C, mutant enzyme Y187S 654650 1.4.1.3 1.25 - 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme 654650 1.4.1.3 1.29 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187E 654650 1.4.1.3 1.31 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187M 654650 1.4.1.3 1.44 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187R 654650 1.4.1.3 1.45 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187G 654650 1.4.1.3 1.46 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187S 654650 1.4.1.3 0.55 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K94Y 654905 1.4.1.3 0.58 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2G96Y 654905 1.4.1.3 0.59 - NAD+ pH 9.5, 25°C, wild-type enzyme hGDH2 654905 1.4.1.3 0.63 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2D172Y 654905 1.4.1.3 0.65 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1G96Y 654905 1.4.1.3 0.71 - NAD+ pH 9.5, 25°C, wild-type enzyme hGDH1 654905 1.4.1.3 0.75 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K130Y 654905 1.4.1.3 0.76 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1D172Y 654905 1.4.1.3 0.79 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K94Y 654905 1.4.1.3 0.82 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K118Y 654905 1.4.1.3 0.92 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K130Y 654905 1.4.1.3 0.94 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K118Y 654905 1.4.1.3 1.77 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2D172Y 654905 1.4.1.3 1.81 - L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH2 654905 1.4.1.3 2.98 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K130Y 654905 1.4.1.3 3.44 - L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH1 654905 1.4.1.3 3.71 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1D172Y 654905 1.4.1.3 5.51 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K130Y 654905 1.4.1.3 7.99 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K94Y 654905 1.4.1.3 10.41 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K118Y 654905 1.4.1.3 17.75 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2G96Y 654905 1.4.1.3 21.82 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K94Y 654905 1.4.1.3 25.82 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K118Y 654905 1.4.1.3 30.55 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1G96Y 654905 1.4.1.3 0.076 - NADH 25°C, mutant enzyme K346L 655569 1.4.1.3 0.079 - NADH 25°C, mutant enzyme K333L 655569 1.4.1.3 0.081 - NADH 25°C, wild-type enzyme 655569 1.4.1.3 0.088 - NADH 25°C, mutant enzyme K337L 655569 1.4.1.3 0.089 - NADH 25°C, mutant enzyme G446D 655569 1.4.1.3 0.09 - NADH 25°C, mutant enzyme K344L 655569 1.4.1.3 0.098 - NADH 25°C, mutant enzyme S445L 655569 1.4.1.3 0.122 - NADH 25°C, mutant enzyme H454Y 655569 1.4.1.3 1.25 - 2-oxoglutarate 25°C, wild-type enzyme 655569 1.4.1.3 1.29 - 2-oxoglutarate 25°C, mutant enzyme K333L 655569 1.4.1.3 1.31 - 2-oxoglutarate 25°C, mutant enzyme K344L 655569 1.4.1.3 1.31 - 2-oxoglutarate 25°C, mutant enzyme K346L 655569 1.4.1.3 1.45 - 2-oxoglutarate 25°C, mutant enzyme K337L 655569 1.4.1.3 1.47 - 2-oxoglutarate 25°C, mutant enzyme S445L 655569 1.4.1.3 1.5 - 2-oxoglutarate 25°C, mutant enzyme G446D 655569 1.4.1.3 3.13 - 2-oxoglutarate 25°C, mutant enzyme H454Y 655569 1.4.1.3 0.081 - NADH pH 8.0, 25°C, wild-type enzyme hGDH1 655582 1.4.1.3 0.086 - NADH pH 8.0, 25°C, wild-type enzyme hGDH2 655582 1.4.1.3 0.088 - NADH pH 8.0, 25°C, mutant enzyme S443R hGDH2 655582 1.4.1.3 1.25 - 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH1 655582 1.4.1.3 1.39 - 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH2 655582 1.4.1.3 1.41 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme S443R hGDH2 655582 1.4.1.3 0.064 - NADH pH 8.0 655623 1.4.1.3 0.42 - NADPH pH 8.0 655623 1.4.1.3 7.1 - 2-oxoglutarate pH 8.0 655623 1.4.1.3 68 - NH3 pH 8.0 655623 1.4.1.3 0.1 - NAD+ pH 8.0, 25°C, recombinant enzyme 655941 1.4.1.3 0.11 - NAD+ pH 8.0, 25°C, native enzyme 655941 1.4.1.3 0.71 - NAD+ pH 9.5, 25°C, wild-type enzyme 656087 1.4.1.3 3.44 - L-glutamate pH 9.5, 25°C, wild-type enzyme 656087 1.4.1.3 3.76 - L-glutamate pH 9.5, 25°C, mutant enzyme E279M 656087 1.4.1.3 3.94 - L-glutamate pH 9.5, 25°C, mutant enzyme E279Y 656087 1.4.1.3 3.98 - L-glutamate pH 9.5, 25°C, mutant enzyme E279G 656087 1.4.1.3 4.05 - L-glutamate pH 9.5, 25°C, mutant enzyme E279L 656087 1.4.1.3 4.12 - L-glutamate pH 9.5, 25°C, mutant enzyme E279R 656087 1.4.1.3 6.94 - NAD+ pH 9.5, 25°C, mutant enzyme E279G 656087 1.4.1.3 7.6 - NAD+ pH 9.5, 25°C, mutant enzyme E279R 656087 1.4.1.3 8.35 - NAD+ pH 9.5, 25°C, mutant enzyme E279Y 656087 1.4.1.3 9.98 - NAD+ pH 9.5, 25°C, mutant enzyme E279M 656087 1.4.1.3 10.01 - NAD+ pH 9.5, 25°C, mutant enzyme E279L 656087 1.4.1.3 additional information - additional information Km-values for 2-oxoglutaratefor mutant enzyme R443S, recombinant wild-type enzyme and wild-type enzyme from human liver 656100 1.4.1.3 additional information - additional information - 656251 1.4.1.3 0.24 - L-glutamate at pH 8.5 671289 1.4.1.3 1.01 - L-glutamate at pH 7.4 671289 1.4.1.3 0.081 - NADH wild-type, hGDH1 673616 1.4.1.3 0.086 - NADH wild-type, hGDH2 673616 1.4.1.3 0.09 - NADH mutant C119A, hGDH1 673616 1.4.1.3 0.091 - NADH mutant C119Y, hGDH1 673616 1.4.1.3 0.092 - NADH mutant C119G, hGDH1 673616 1.4.1.3 0.094 - NADH mutant C59G, hGDH1 673616 1.4.1.3 0.097 - NADH mutant C59A, hGDH1 673616 1.4.1.3 0.098 - NADH mutant C119G, hGDH2 673616 1.4.1.3 0.099 - NADH mutant C59A, hGDH2 673616 1.4.1.3 0.099 - NADH mutant C59Y, hGDH1 673616 1.4.1.3 0.101 - NADH mutant C119A, hGDH2 673616 1.4.1.3 0.102 - NADH mutant C59Y, hGDH2 673616 1.4.1.3 0.106 - NADH mutant C59G, hGDH2 673616 1.4.1.3 0.108 - NADH mutant C119Y, hGDH2 673616 1.4.1.3 0.169 - NADH mutant C274A, hGDH2 673616 1.4.1.3 0.175 - NADH mutant C274G, hGDH2 673616 1.4.1.3 0.176 - NADH mutant C274Y, hGDH2 673616 1.4.1.3 0.178 - NADH mutant C274Y, hGDH1 673616 1.4.1.3 0.181 - NADH mutant C274G, hGDH1 673616 1.4.1.3 0.189 - NADH mutant C274A, hGDH1 673616 1.4.1.3 1.16 - 2-oxoglutarate mutant C274G, hGDH1 673616 1.4.1.3 1.25 - 2-oxoglutarate wild-type, hGDH1 673616 1.4.1.3 1.34 - 2-oxoglutarate mutant C59G, hGDH1 673616 1.4.1.3 1.36 - 2-oxoglutarate mutant C59Y, hGDH1 673616 1.4.1.3 1.37 - 2-oxoglutarate mutant C274Y, hGDH1 673616 1.4.1.3 1.39 - 2-oxoglutarate wild-type, hGDH2 673616 1.4.1.3 1.4 - 2-oxoglutarate mutant C274A, hGDH1 673616 1.4.1.3 1.4 - 2-oxoglutarate mutant C59A, hGDH1 673616 1.4.1.3 1.58 - 2-oxoglutarate mutant C274G, hGDH2 673616 1.4.1.3 1.58 - 2-oxoglutarate mutant C59Y, hGDH2 673616 1.4.1.3 1.59 - 2-oxoglutarate mutant C274A, hGDH2 673616 1.4.1.3 1.61 - 2-oxoglutarate mutant C59A, hGDH2 673616 1.4.1.3 1.66 - 2-oxoglutarate mutant C274Y, hGDH2 673616 1.4.1.3 1.67 - 2-oxoglutarate mutant C59G, hGDH2 673616 1.4.1.3 1.86 - 2-oxoglutarate mutant C119Y, hGDH1 673616 1.4.1.3 1.9 - 2-oxoglutarate mutant C119A, hGDH1 673616 1.4.1.3 1.94 - 2-oxoglutarate mutant C119G, hGDH1 673616 1.4.1.3 2.12 - 2-oxoglutarate mutant C119A, hGDH2 673616 1.4.1.3 2.18 - 2-oxoglutarate mutant C119G, hGDH2 673616 1.4.1.3 2.26 - 2-oxoglutarate mutant C119Y, hGDH2 673616 1.4.1.3 0.081 - NADH hGDH1, wild-type 675933 1.4.1.3 0.086 - NADH hGDH2, wild-type 675933 1.4.1.3 0.117 - NADH hGDH1, mutant C323L 675933 1.4.1.3 0.119 - NADH hGDH1, mutant C323G 675933 1.4.1.3 0.119 - NADH hGDH1, mutant C323M 675933 1.4.1.3 0.119 - NADH hGDH2, mutant C323M 675933 1.4.1.3 0.121 - NADH hGDH2, mutant C323L 675933 1.4.1.3 0.122 - NADH hGDH1, mutant C323R 675933 1.4.1.3 0.125 - NADH hGDH2, mutant C323R 675933 1.4.1.3 0.128 - NADH hGDH2, mutant C323G 675933 1.4.1.3 0.129 - NADH hGDH1, mutant C323Y 675933 1.4.1.3 0.138 - NADH hGDH2, mutant C323Y 675933 1.4.1.3 1.25 - 2-oxoglutarate hGDH1, wild-type 675933 1.4.1.3 1.34 - 2-oxoglutarate hGDH1, mutant C323L 675933 1.4.1.3 1.36 - 2-oxoglutarate hGDH1, mutant C323Y 675933 1.4.1.3 1.38 - 2-oxoglutarate hGDH1, mutant C323M 675933 1.4.1.3 1.39 - 2-oxoglutarate hGDH1, mutant C323R 675933 1.4.1.3 1.39 - 2-oxoglutarate hGDH2, wild-type 675933 1.4.1.3 1.4 - 2-oxoglutarate hGDH1, mutant C323G 675933 1.4.1.3 1.41 - 2-oxoglutarate hGDH2, mutant C323Y 675933 1.4.1.3 1.45 - 2-oxoglutarate hGDH2, mutant C323L 675933 1.4.1.3 1.45 - 2-oxoglutarate hGDH2, mutant C323M 675933 1.4.1.3 1.5 - 2-oxoglutarate hGDH2, mutant C323R 675933 1.4.1.3 1.5 2 2-oxoglutarate hGDH2, mutant C323G 675933 1.4.1.3 0.01 - NADPH pH 7.6, 60°C 679617 1.4.1.3 0.14 - NADH pH 7.6, 50°C 679617 1.4.1.3 1.4 - 2-oxoglutarate pH 7.6, 60°C 679617 1.4.1.3 2.5 - L-glutamate pH 7.6, 60°C 679617 1.4.1.3 0.02 - NAD+ in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 0.035 - NADP+ in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 0.04 - NADH in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 3 - L-glutamate in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 3.5 - 2-oxoglutarate in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 10 - NH3 in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 0.075 - NADH mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.08 - NADH mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.081 - NADH wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.086 - NADH wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.25 - 2-oxoglutarate wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.27 - 2-oxoglutarate mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.29 - 2-oxoglutarate mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.39 - 2-oxoglutarate wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.081 - NADH isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 0.086 - NADH isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 1.25 - 2-oxoglutarate isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 1.39 - 2-oxoglutarate isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 1.5 - 2-oxoglutarate mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 2 - 2-oxoglutarate wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 2.1 - 2-oxoglutarate wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 10.7 - L-glutamate wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 10.8 - L-glutamate mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 12.4 - L-glutamate wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 13.4 - NH3 wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 17.1 - NH3 wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 22.2 - NH3 mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 additional information - additional information kinetic analysis in wild-type and transgenic overexpressing brain cell fractions, overview 712827 1.4.1.3 0.04 - NADPH pH 7.6, 70°C, native enzyme 724160 1.4.1.3 0.05 - NADPH pH 7.6, 70°C, recombinent enzyme (heated) 724160 1.4.1.3 0.1 - NADPH pH 7.6, 70°C, recombinent enzyme (unheated) 724160 1.4.1.3 0.2 - 2-oxoglutarate pH 7.6, 70°C, recombinent enzyme (unheated) 724160 1.4.1.3 0.25 - 2-oxoglutarate pH 7.6, 70°C, native enzyme 724160 1.4.1.3 0.3 - 2-oxoglutarate pH 7.6, 70°C, recombinent enzyme (heated) 724160 1.4.1.3 2.5 - NH3 pH 7.6, 70°C, recombinent enzyme (unheated) 724160 1.4.1.3 11.4 - NH3 pH 7.6, 70°C, recombinent enzyme (heated) 724160 1.4.1.3 13 - NH3 pH 7.6, 70°C, native enzyme 724160 1.4.1.3 0.1 - NADP+ pH 8.0, 70°C 724653 1.4.1.3 0.3 - L-glutamate pH 8.0, 70°C 724653 1.4.1.3 0.4 - NAD+ pH 8.0, 70°C 724653 1.4.1.3 additional information - additional information lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination 725866 1.4.1.3 12.8 - NH3 hGDH1, pH 8.0, temperature not specified in the publication 725866 1.4.1.3 14.7 - NH3 hGDH2, pH 8.0, temperature not specified in the publication 725866 1.4.1.3 33 - NH3 hGDH2, pH 7.5, temperature not specified in the publication 725866 1.4.1.3 35 - NH3 hGDH1, pH 7.5, temperature not specified in the publication 725866 1.4.1.3 57.5 - NH3 hGDH1, pH 7.0, temperature not specified in the publication 725866 1.4.1.3 62.2 - NH3 hGDH2, pH 7.0, temperature not specified in the publication 725866 1.4.1.3 0.003 - NADH recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.0051 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 0.0053 - NADH recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.0058 - NADH recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.008 - NADH recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 0.0229 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.024 - NAD+ recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 0.027 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.052 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.056 - NAD+ recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.13 - NAD+ recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.16 - NAD+ recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.27 - L-glutamate recombinant enzyme complex TtGDH-APRTh, low Glu concentration, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.45 - L-glutamate recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.77 - L-glutamate recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 1.2 - L-glutamate recombinant enzyme complex TtGDH-APRTh, low Glu concentration, pH and temperature not specified in the publication 759432 1.4.1.3 19 - L-glutamate recombinant enzyme complex TtGDH-APRTh, high Glu concentration, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 29 - NH3 recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 33 - NH3 recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 43 - NH3 recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 46 - NH3 recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 52 - L-glutamate recombinant enzyme complex TtGDH-APRTh, high Glu concentration, pH and temperature not specified in the publication 759432 1.4.1.3 additional information - additional information when assayed in triethanolamine buffer, pH 8.0, at 1.0 mM ADP, hGDH1 and hGDH2 show similar catalytic properties (Vmax and Kms for 2-oxoglutarate, ammonia and glutamate), kinetics, overview 762783