1.4.1.3 additional information - additional information - 656251 1.4.1.3 additional information - additional information kinetic analysis in wild-type and transgenic overexpressing brain cell fractions, overview 712827 1.4.1.3 additional information - additional information Km-values for 2-oxoglutaratefor mutant enzyme R443S, recombinant wild-type enzyme and wild-type enzyme from human liver 656100 1.4.1.3 additional information - additional information lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination 725866 1.4.1.3 additional information - additional information when assayed in triethanolamine buffer, pH 8.0, at 1.0 mM ADP, hGDH1 and hGDH2 show similar catalytic properties (Vmax and Kms for 2-oxoglutarate, ammonia and glutamate), kinetics, overview 762783 1.4.1.3 0.000175 - NADH - 391594 1.4.1.3 0.00294 - alpha-ketoglutarate - 391594 1.4.1.3 0.003 - NADH recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.004 - NADH 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.004 - NADP+ NADP+-dependent activity 391615 1.4.1.3 0.0051 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 0.0053 - NADH recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.0058 - NADH recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.006 - NADPH NADPH-dependent amination 391619 1.4.1.3 0.007 - NADH + 2-oxoglutarate 391621 1.4.1.3 0.008 - NADH recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 0.0083 - NADH - 391631 1.4.1.3 0.009 - NADH glutamate dehydrogenase 1, 5 mM 2-oxoglutarate 391607 1.4.1.3 0.009 - NADH glutamate dehydrogenase 2, 5 mM 2-oxoglutarate 391607 1.4.1.3 0.01 - NADH 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 0.01 - NADPH + 2-oxoglutarate 391621 1.4.1.3 0.01 - NADPH pH 7.6, 60°C 679617 1.4.1.3 0.011 - NADPH - 391631 1.4.1.3 0.011 - NADPH reductive amination, low-activity form of the enzyme 391620 1.4.1.3 0.012 - NADH 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.012 - NADH glutamate dehydrogenase 2, 80 mM NH4+ 391607 1.4.1.3 0.013 - NADP+ oxidative deamination, low-activity form of the enzyme 391620 1.4.1.3 0.013 - NADPH NADP-linked reductive amination 391618 1.4.1.3 0.013 - NADPH NADPH-dependent activity 391615 1.4.1.3 0.013 - NADPH reductive amination, high-activity form of the enzyme 391620 1.4.1.3 0.014 - NAD+ - 391631 1.4.1.3 0.015 - NADH liver enzyme 391624 1.4.1.3 0.016 - NAD+ 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.018 - NADPH NADPH-dependent amination 391619 1.4.1.3 0.019 - NADP+ NADP-linked oxidative deamination 391618 1.4.1.3 0.02 - NAD+ in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 0.02 - NADH - 391614 1.4.1.3 0.02 - NADH glutamate dehydrogenase 1, 40 mM NH4+ 391607 1.4.1.3 0.02 - NADPH - 391614 1.4.1.3 0.02 - NADPH NADPH-dependent amination 391619 1.4.1.3 0.022 - NADH kidney enzyme 391624 1.4.1.3 0.022 - NADPH - 391612 1.4.1.3 0.0229 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.024 - NAD+ recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 0.025 - NADP+ + L-glutamate 391621 1.4.1.3 0.025 - NADP+ pH 7.6, 90°C 654356 1.4.1.3 0.027 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.028 - NADP+ cosubstrate glutamate 391612 1.4.1.3 0.028 - NADPH glutamate dehydrogenase 1, 10 mM 2-oxoglutarate 391607 1.4.1.3 0.028 - NADPH glutamate dehydrogenase 2, 10 mM 2-oxoglutarate 391607 1.4.1.3 0.029 - NADP+ oxidative deamination, high-activity form of the enzyme 391620 1.4.1.3 0.032 - NAD+ kidney enzyme 391624 1.4.1.3 0.035 - NADP+ in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 0.037 - NADPH pH 7.6, 90°C 654356 1.4.1.3 0.04 - NADH in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 0.04 - NADPH concentration range: 0.002-0.1 mM 391632 1.4.1.3 0.04 - NADPH pH 7.6, 70°C, native enzyme 724160 1.4.1.3 0.0424 - NH4+ - 391594 1.4.1.3 0.048 - NADH glutamate dehydrogenase 1, 400 mM NH4+ 391607 1.4.1.3 0.05 - NADH glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate 391607 1.4.1.3 0.05 - NADPH pH 7.6, 70°C, recombinent enzyme (heated) 724160 1.4.1.3 0.052 - 2-oxoglutarate recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.052 - N6-(3-sulfonatopropyl)-NAD+ - 391617 1.4.1.3 0.056 - NAD+ recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.063 - NAD+ + L-glutamate 391621 1.4.1.3 0.064 - NADH pH 8.0 655623 1.4.1.3 0.069 - NADPH glutamate dehydrogenase 1, 40 mM NH4+ 391607 1.4.1.3 0.069 - NADPH glutamate dehydrogenase 2, 80 mM NH4+ 391607 1.4.1.3 0.07 - NADH isozyme 1, amination activity, cofactor NADH 391622 1.4.1.3 0.075 - NADH mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.076 - NADH 25°C, mutant enzyme K346L 655569 1.4.1.3 0.079 - NADH 25°C, mutant enzyme K333L 655569 1.4.1.3 0.08 - NADH mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.08 - NADH pH 8.0, 25°C, wild-type enzyme 654650 1.4.1.3 0.08 - NADP+ concentration range: 0.02-0.2 mM 391632 1.4.1.3 0.081 - NADH 25°C, wild-type enzyme 655569 1.4.1.3 0.081 - NADH hGDH1, wild-type 675933 1.4.1.3 0.081 - NADH isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 0.081 - NADH pH 8.0, 25°C, wild-type enzyme hGDH1 655582 1.4.1.3 0.081 - NADH wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.081 - NADH wild-type, hGDH1 673616 1.4.1.3 0.082 - NADH pH 8.0, 25°C, mutant enzyme Y187E 654650 1.4.1.3 0.082 - NADH pH 8.0, 25°C, mutant enzyme Y187R 654650 1.4.1.3 0.083 - NADH pH 7.6, 90°C 654356 1.4.1.3 0.084 - NADH pH 8.0, 25°C, mutant enzyme Y187M 654650 1.4.1.3 0.085 - NADH pH 8.0, 25°C, mutant enzyme Y187G 654650 1.4.1.3 0.086 - NADH hGDH2, wild-type 675933 1.4.1.3 0.086 - NADH isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 0.086 - NADH pH 8.0, 25°C, wild-type enzyme hGDH2 655582 1.4.1.3 0.086 - NADH wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 0.086 - NADH wild-type, hGDH2 673616 1.4.1.3 0.088 - NADH 25°C, mutant enzyme K337L 655569 1.4.1.3 0.088 - NADH pH 8.0, 25°C, mutant enzyme S443R hGDH2 655582 1.4.1.3 0.088 - NADH pH 8.0, 25°C, mutant enzyme Y187S 654650 1.4.1.3 0.089 - NADH 25°C, mutant enzyme G446D 655569 1.4.1.3 0.09 - NADH 25°C, mutant enzyme K344L 655569 1.4.1.3 0.09 - NADH concentration range: 0.04-0.1 mM 391632 1.4.1.3 0.09 - NADH isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 0.09 - NADH mutant C119A, hGDH1 673616 1.4.1.3 0.091 - NADH mutant C119Y, hGDH1 673616 1.4.1.3 0.092 - NADH mutant C119G, hGDH1 673616 1.4.1.3 0.094 - NADH mutant C59G, hGDH1 673616 1.4.1.3 0.097 - NADH mutant C59A, hGDH1 673616 1.4.1.3 0.098 - NADH 25°C, mutant enzyme S445L 655569 1.4.1.3 0.098 - NADH mutant C119G, hGDH2 673616 1.4.1.3 0.099 - NADH mutant C59A, hGDH2 673616 1.4.1.3 0.099 - NADH mutant C59Y, hGDH1 673616 1.4.1.3 0.1 - 2-oxoglutarate euthermic animal, assay at 37°C 391636 1.4.1.3 0.1 - NAD+ pH 8.0, 25°C, recombinant enzyme 655941 1.4.1.3 0.1 - NADP+ pH 8.0, 70°C 724653 1.4.1.3 0.1 - NADPH pH 7.6, 70°C, recombinent enzyme (unheated) 724160 1.4.1.3 0.101 - NADH mutant C119A, hGDH2 673616 1.4.1.3 0.102 - NADH mutant C59Y, hGDH2 673616 1.4.1.3 0.106 - NADH mutant C59G, hGDH2 673616 1.4.1.3 0.108 - NADH mutant C119Y, hGDH2 673616 1.4.1.3 0.11 - NAD+ 500 mM Tris-HCl, pH 9.4 391609 1.4.1.3 0.11 - NAD+ pH 8.0, 25°C, native enzyme 655941 1.4.1.3 0.11 - NADH - 391604 1.4.1.3 0.117 - NADH hGDH1, mutant C323L 675933 1.4.1.3 0.119 - NADH hGDH1, mutant C323G 675933 1.4.1.3 0.119 - NADH hGDH1, mutant C323M 675933 1.4.1.3 0.119 - NADH hGDH2, mutant C323M 675933 1.4.1.3 0.121 - NADH hGDH2, mutant C323L 675933 1.4.1.3 0.122 - NADH 25°C, mutant enzyme H454Y 655569 1.4.1.3 0.122 - NADH hGDH1, mutant C323R 675933 1.4.1.3 0.125 - NADH hGDH2, mutant C323R 675933 1.4.1.3 0.128 - NADH hGDH2, mutant C323G 675933 1.4.1.3 0.129 - NADH hGDH1, mutant C323Y 675933 1.4.1.3 0.13 - NAD+ recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.13 - NADH reductive amination, low-activity form of the enzyme 391620 1.4.1.3 0.13 - NADPH - 391604 1.4.1.3 0.138 - NADH hGDH2, mutant C323Y 675933 1.4.1.3 0.14 - 2-oxoglutarate NADP-linked reductive amination 391618 1.4.1.3 0.14 - NADH isozyme 2, amination activity, cofactor NADH 391622 1.4.1.3 0.14 - NADH pH 7.6, 50°C 679617 1.4.1.3 0.148 - N6-(2-hydroxy-3-trimethylammoniumpropyl)-NAD+ - 391617 1.4.1.3 0.15 - NAD+ 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.16 - NAD+ recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.16 - NADP+ - 391617 1.4.1.3 0.169 - NADH mutant C274A, hGDH2 673616 1.4.1.3 0.17 - NADPH 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.175 - NAD+ - 391617 1.4.1.3 0.175 - NADH mutant C274G, hGDH2 673616 1.4.1.3 0.176 - NADH mutant C274Y, hGDH2 673616 1.4.1.3 0.178 - NADH mutant C274Y, hGDH1 673616 1.4.1.3 0.18 - 2-oxoglutarate reductive amination, low-activity form of the enzyme 391620 1.4.1.3 0.18 - NADP+ substrate L-glutamate 391623 1.4.1.3 0.181 - NADH mutant C274G, hGDH1 673616 1.4.1.3 0.189 - NADH mutant C274A, hGDH1 673616 1.4.1.3 0.2 - 2-oxoglutarate cosubstrate NADPH 391621 1.4.1.3 0.2 - 2-oxoglutarate pH 7.6, 70°C, recombinent enzyme (unheated) 724160 1.4.1.3 0.2 - 2-oxoglutarate reductive amination, high-activity form of the enzyme 391620 1.4.1.3 0.2 - NAD+ isozyme 1, deamination activity 391622 1.4.1.3 0.2 - NADH NAD-linked reductive amination 391618 1.4.1.3 0.24 - L-glutamate at pH 8.5 671289 1.4.1.3 0.25 - 2-oxoglutarate concentration range: 0.3-4.0 mM 391632 1.4.1.3 0.25 - 2-oxoglutarate NADPH-dependent activity 391615 1.4.1.3 0.25 - 2-oxoglutarate pH 7.6, 70°C, native enzyme 724160 1.4.1.3 0.25 - glutamate hibernating animal, assay at 5°C 391636 1.4.1.3 0.26 - NAD+ isozyme 2, deamination activity 391622 1.4.1.3 0.27 - L-glutamate recombinant enzyme complex TtGDH-APRTh, low Glu concentration, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.27 - NADPH isozyme 1, amination activity, cofactor NADPH 391622 1.4.1.3 0.273 - N6-(2-aminoethyl)-NADP+ - 391617 1.4.1.3 0.291 - N6-(2-aminoethyl)-NAD+ - 391617 1.4.1.3 0.3 - 2-oxoglutarate pH 7.6, 70°C, recombinent enzyme (heated) 724160 1.4.1.3 0.3 - L-glutamate cosubstrate NAD+ 391621 1.4.1.3 0.3 - L-glutamate pH 8.0, 70°C 724653 1.4.1.3 0.31 - NADP+ cosubstrate norvaline 391612 1.4.1.3 0.32 - glutamate concentration range: 0.05-1.0 mM 391632 1.4.1.3 0.32 - NAD+ isozyme 3, deamination activity 391622 1.4.1.3 0.36 - 2-oxoglutarate isozyme 1, amination activity, cofactor NADH 391622 1.4.1.3 0.36 - 2-oxoglutarate NADH + NH4Cl 391614 1.4.1.3 0.364 - NAD+ mitochondrial enzyme 391634 1.4.1.3 0.38 - NH3 biphasic Lineweaver-Burk plot suggests 2 Km values 391615 1.4.1.3 0.4 - 2-oxoglutarate 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.4 - L-glutamate kidney enzyme 391624 1.4.1.3 0.4 - NAD+ pH 8.0, 70°C 724653 1.4.1.3 0.42 - NADPH pH 8.0 655623 1.4.1.3 0.425 - poly(ethyleneglycol)-N6-(2-aminoethyl)-NADP+ - 391617 1.4.1.3 0.43 - 2-oxoglutarate hibernating animal, assay at 5°C 391636 1.4.1.3 0.43 - NAD+ oxidative deamination, low-activity form of the enzyme 391620 1.4.1.3 0.443 - NADP+ enzyme from rough ER 391634 1.4.1.3 0.444 - poly(ethyleneglycol)-N6-(2-aminoethyl)-NAD+ - 391617 1.4.1.3 0.45 - L-glutamate recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 0.46 - NAD+ - 391604 1.4.1.3 0.46 - NADPH isozyme 3, amination activity, cofactor NADPH 391622 1.4.1.3 0.47 - 2-oxoglutarate NADPH + NH4Cl 391614 1.4.1.3 0.47 - glutamate assay at 5°C 391636 1.4.1.3 0.5 - glutamate euthermic animal, assay at 5°C 391636 1.4.1.3 0.5 - NADH NADH-dependent activity 391615 1.4.1.3 0.52 - NADH reductive amination, high-activity form of the enzyme 391620 1.4.1.3 0.53 - 2-oxoglutarate pH 7.6, 90°C 654356 1.4.1.3 0.53 - NAD+ substrate L-glutamate 391623 1.4.1.3 0.54 - NADPH 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 0.55 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K94Y 654905 1.4.1.3 0.56 - NADPH substrate 2-oxoglutarate 391623 1.4.1.3 0.56 - NADPH substrate NH3 391623 1.4.1.3 0.58 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2G96Y 654905 1.4.1.3 0.59 - 2-oxoglutarate isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 0.59 - NAD+ pH 9.5, 25°C, wild-type enzyme hGDH2 654905 1.4.1.3 0.6 - 2-oxoglutarate cosubstrate NADH 391621 1.4.1.3 0.6 - L-glutamate pH 7.6, 90°C 654356 1.4.1.3 0.62 - 2-oxoglutarate glutamate dehydrogenase 2, 0.1 mM NADH 391607 1.4.1.3 0.63 - 2-oxoglutarate NADPH-dependent amination 391619 1.4.1.3 0.63 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2D172Y 654905 1.4.1.3 0.637 - NADP+ mitochondrial enzyme 391634 1.4.1.3 0.64 - 2-oxoglutarate NADPH-dependent amination 391619 1.4.1.3 0.64 - L-glutamate isozyme 1, deamination activity 391622 1.4.1.3 0.65 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1G96Y 654905 1.4.1.3 0.7 - 2-oxoglutarate cofactor NADH 391631 1.4.1.3 0.7 - 2-oxoglutarate NADPH-dependent amination 391619 1.4.1.3 0.71 - NAD+ pH 9.5, 25°C, wild-type enzyme 656087 1.4.1.3 0.71 - NAD+ pH 9.5, 25°C, wild-type enzyme hGDH1 654905 1.4.1.3 0.74 - L-glutamate - 391612 1.4.1.3 0.75 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K130Y 654905 1.4.1.3 0.75 - NADPH 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 0.76 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1D172Y 654905 1.4.1.3 0.77 - L-glutamate recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 0.78 - NADPH isozyme 2, amination activity, cofactor NADPH 391622 1.4.1.3 0.79 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K94Y 654905 1.4.1.3 0.82 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K118Y 654905 1.4.1.3 0.92 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K130Y 654905 1.4.1.3 0.924 - NAD+ enzyme from rough ER 391634 1.4.1.3 0.94 - NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K118Y 654905 1.4.1.3 0.98 - 2-oxoglutarate hibernating animal, assay at 37°C 391636 1.4.1.3 0.98 - NADH substrate 2-oxoglutarate 391623 1.4.1.3 1 - 2-oxoglutarate liver enzyme 391624 1.4.1.3 1 - L-glutamate 1 mM, NAD+ 391614 1.4.1.3 1.01 - L-glutamate at pH 7.4 671289 1.4.1.3 1.1 - 2-oxoglutarate glutamate dehydrogenase 2, 80 mM NH4+ 391607 1.4.1.3 1.1 - L-glutamate cosubstrate NADP+ 391621 1.4.1.3 1.16 - 2-oxoglutarate mutant C274G, hGDH1 673616 1.4.1.3 1.2 - 2-oxoglutarate 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 1.2 - L-glutamate recombinant enzyme complex TtGDH-APRTh, low Glu concentration, pH and temperature not specified in the publication 759432 1.4.1.3 1.23 - 2-oxoglutarate isozyme 2, amination activity, cofactor NADH 391622 1.4.1.3 1.25 - 2-oxoglutarate 25°C, wild-type enzyme 655569 1.4.1.3 1.25 - 2-oxoglutarate hGDH1, wild-type 675933 1.4.1.3 1.25 - 2-oxoglutarate isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 1.25 - 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme 654650 1.4.1.3 1.25 - 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH1 655582 1.4.1.3 1.25 - 2-oxoglutarate wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.25 - 2-oxoglutarate wild-type, hGDH1 673616 1.4.1.3 1.25 - NH3 reductive amination, high-activity form of the enzyme 391620 1.4.1.3 1.27 - 2-oxoglutarate mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.29 - 2-oxoglutarate 25°C, mutant enzyme K333L 655569 1.4.1.3 1.29 - 2-oxoglutarate mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.29 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187E 654650 1.4.1.3 1.3 - NH4+ concentration range: 0.2-5 mM 391632 1.4.1.3 1.31 - 2-oxoglutarate 25°C, mutant enzyme K344L 655569 1.4.1.3 1.31 - 2-oxoglutarate 25°C, mutant enzyme K346L 655569 1.4.1.3 1.31 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187M 654650 1.4.1.3 1.31 - NH3 reductive amination, low-activity form of the enzyme 391620 1.4.1.3 1.34 - 2-oxoglutarate hGDH1, mutant C323L 675933 1.4.1.3 1.34 - 2-oxoglutarate mutant C59G, hGDH1 673616 1.4.1.3 1.36 - 2-oxoglutarate hGDH1, mutant C323Y 675933 1.4.1.3 1.36 - 2-oxoglutarate mutant C59Y, hGDH1 673616 1.4.1.3 1.37 - 2-oxoglutarate mutant C274Y, hGDH1 673616 1.4.1.3 1.38 - 2-oxoglutarate hGDH1, mutant C323M 675933 1.4.1.3 1.39 - 2-oxoglutarate hGDH1, mutant C323R 675933 1.4.1.3 1.39 - 2-oxoglutarate hGDH2, wild-type 675933 1.4.1.3 1.39 - 2-oxoglutarate isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C 687565 1.4.1.3 1.39 - 2-oxoglutarate pH 8.0, 25°C, wild-type enzyme hGDH2 655582 1.4.1.3 1.39 - 2-oxoglutarate wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C 684905 1.4.1.3 1.39 - 2-oxoglutarate wild-type, hGDH2 673616 1.4.1.3 1.4 - 2-oxoglutarate - 391636 1.4.1.3 1.4 - 2-oxoglutarate assay at 37°C 391636 1.4.1.3 1.4 - 2-oxoglutarate hGDH1, mutant C323G 675933 1.4.1.3 1.4 - 2-oxoglutarate mutant C274A, hGDH1 673616 1.4.1.3 1.4 - 2-oxoglutarate mutant C59A, hGDH1 673616 1.4.1.3 1.4 - 2-oxoglutarate pH 7.6, 60°C 679617 1.4.1.3 1.41 - 2-oxoglutarate hGDH2, mutant C323Y 675933 1.4.1.3 1.41 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme S443R hGDH2 655582 1.4.1.3 1.44 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187R 654650 1.4.1.3 1.45 - 2-oxoglutarate 25°C, mutant enzyme K337L 655569 1.4.1.3 1.45 - 2-oxoglutarate hGDH2, mutant C323L 675933 1.4.1.3 1.45 - 2-oxoglutarate hGDH2, mutant C323M 675933 1.4.1.3 1.45 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187G 654650 1.4.1.3 1.46 - 2-oxoglutarate pH 8.0, 25°C, mutant enzyme Y187S 654650 1.4.1.3 1.47 - 2-oxoglutarate 25°C, mutant enzyme S445L 655569 1.4.1.3 1.5 - 2-oxoglutarate 25°C, mutant enzyme G446D 655569 1.4.1.3 1.5 - 2-oxoglutarate hGDH2, mutant C323R 675933 1.4.1.3 1.5 - 2-oxoglutarate mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 1.5 - NH3 NADPH-dependent amination 391619 1.4.1.3 1.5 2 2-oxoglutarate hGDH2, mutant C323G 675933 1.4.1.3 1.51 - 2-oxoglutarate glutamate dehydrogenase 1, 0.1 mM NADH 391607 1.4.1.3 1.51 - 2-oxoglutarate glutamate dehydrogenase 1, 400 mM NH4+ 391607 1.4.1.3 1.54 - 2-oxoglutarate isozyme 1, amination activity, cofactor NADPH 391622 1.4.1.3 1.58 - 2-oxoglutarate mutant C274G, hGDH2 673616 1.4.1.3 1.58 - 2-oxoglutarate mutant C59Y, hGDH2 673616 1.4.1.3 1.59 - 2-oxoglutarate mutant C274A, hGDH2 673616 1.4.1.3 1.6 - L-glutamate cofactor NADP+ 391623 1.4.1.3 1.61 - 2-oxoglutarate mutant C59A, hGDH2 673616 1.4.1.3 1.66 - 2-oxoglutarate mutant C274Y, hGDH2 673616 1.4.1.3 1.66 - L-glutamate cofactor NAD+ 391623 1.4.1.3 1.67 - 2-oxoglutarate mutant C59G, hGDH2 673616 1.4.1.3 1.7 - L-glutamate 50 mM Tris-HCl, pH 7.2 391609 1.4.1.3 1.7 - NH3 NADP-linked reductive amination 391618 1.4.1.3 1.73 - 2-oxoglutarate isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 1.77 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2D172Y 654905 1.4.1.3 1.81 - L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH2 654905 1.4.1.3 1.85 - 2-oxoglutarate isozyme 2, amination activity, cofactor NADPH 391622 1.4.1.3 1.86 - 2-oxoglutarate mutant C119Y, hGDH1 673616 1.4.1.3 1.9 - 2-oxoglutarate mutant C119A, hGDH1 673616 1.4.1.3 1.94 - 2-oxoglutarate mutant C119G, hGDH1 673616 1.4.1.3 2 - 2-oxoglutarate wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 2.03 - glutamate euthermic animal, assay at 37°C 391636 1.4.1.3 2.1 - 2-oxoglutarate cosubstrate NADPH 391604 1.4.1.3 2.1 - 2-oxoglutarate glutamate dehydrogenase 1, 40 mM NH4+ 391607 1.4.1.3 2.1 - 2-oxoglutarate wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 2.1 - glutamate - 391631 1.4.1.3 2.12 - 2-oxoglutarate mutant C119A, hGDH2 673616 1.4.1.3 2.18 - 2-oxoglutarate mutant C119G, hGDH2 673616 1.4.1.3 2.26 - 2-oxoglutarate mutant C119Y, hGDH2 673616 1.4.1.3 2.3 - 2-oxoglutarate kidney enzyme 391624 1.4.1.3 2.4 - 2-oxoglutarate - 391612 1.4.1.3 2.4 - L-glutamate NADP-linked oxidative deamination 391618 1.4.1.3 2.4 - NH3 NADPH-dependent amination 391619 1.4.1.3 2.44 - NAD+ oxidative deamination, high-activity form of the enzyme 391620 1.4.1.3 2.5 - L-glutamate pH 7.6, 60°C 679617 1.4.1.3 2.5 - NH3 pH 7.6, 70°C, recombinent enzyme (unheated) 724160 1.4.1.3 2.6 - 2-oxoglutarate glutamate dehydrogenase 1, 0.2 mM NADPH 391607 1.4.1.3 2.6 - glutamate assay at 37°C 391636 1.4.1.3 2.82 - NH4+ pH 7.6, 90°C 654356 1.4.1.3 2.9 - NH3 NADPH-dependent amination 391619 1.4.1.3 2.98 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K130Y 654905 1.4.1.3 3 - L-glutamate 1 mM, NADP+ 391614 1.4.1.3 3 - L-glutamate in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 3 - L-glutamate isozyme 3, deamination activity 391622 1.4.1.3 3 - NAD+ NAD-linked oxidative deamination 391618 1.4.1.3 3.13 - 2-oxoglutarate 25°C, mutant enzyme H454Y 655569 1.4.1.3 3.2 - NH4+ - 391602 1.4.1.3 3.3 - 2-oxoglutarate cosubstrate NADH 391604 1.4.1.3 3.44 - L-glutamate pH 9.5, 25°C, wild-type enzyme 656087 1.4.1.3 3.44 - L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH1 654905 1.4.1.3 3.5 - 2-oxoglutarate in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 3.52 - L-glutamate isozyme 2, deamination activity 391622 1.4.1.3 3.65 - L-glutamate oxidative deamination, low-activity form of the enzyme 391620 1.4.1.3 3.66 - 2-oxoglutarate euthermic animal, assay at 5°C 391636 1.4.1.3 3.7 - 2-oxoglutarate glutamate dehydrogenase 1, 0.2 mM NADH 391607 1.4.1.3 3.7 - 2-oxoglutarate glutamate dehydrogenase 2, 0.2 mM NADPH 391607 1.4.1.3 3.71 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1D172Y 654905 1.4.1.3 3.76 - L-glutamate pH 9.5, 25°C, mutant enzyme E279M 656087 1.4.1.3 3.83 - L-glutamate oxidative deamination, high-activity form of the enzyme 391620 1.4.1.3 3.94 - L-glutamate pH 9.5, 25°C, mutant enzyme E279Y 656087 1.4.1.3 3.98 - L-glutamate pH 9.5, 25°C, mutant enzyme E279G 656087 1.4.1.3 4 - 2-oxoglutarate cofactor NADPH 391623 1.4.1.3 4.05 - L-glutamate pH 9.5, 25°C, mutant enzyme E279L 656087 1.4.1.3 4.1 - NH4+ - 391636 1.4.1.3 4.1 - NH4+ assay at 5°C 391636 1.4.1.3 4.12 - L-glutamate pH 9.5, 25°C, mutant enzyme E279R 656087 1.4.1.3 4.5 - 2-oxoglutarate cofactor NADH 391623 1.4.1.3 4.61 - glutamate mitochondrial enzyme, cofactor NAD+ 391634 1.4.1.3 4.9 - NH3 NAD-linked reductive amination 391618 1.4.1.3 5 - 2-oxoglutarate NADH-dependent activity 391615 1.4.1.3 5.1 - NH3 NADP-linked reductive amination 391618 1.4.1.3 5.2 - glutamate hibernating animal, assay at 37°C 391636 1.4.1.3 5.5 - glutamate concentration range: 1.0-10.0 mM 391632 1.4.1.3 5.51 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K130Y 654905 1.4.1.3 5.8 - NH4+ glutamate dehydrogenase 1, 0.2 mM NADPH 391607 1.4.1.3 5.93 - glutamate enzyme from rough ER, cofactor NAD+ 391634 1.4.1.3 6.3 - 2-oxoglutarate cofactor NADPH 391631 1.4.1.3 6.5 - NH4+ - 391612 1.4.1.3 6.94 - NAD+ pH 9.5, 25°C, mutant enzyme E279G 656087 1.4.1.3 7 - NH4+ euthermic animal, assay at 37°C 391636 1.4.1.3 7 - NH4+ glutamate dehydrogenase 1, 0.2 mM NADPH 391607 1.4.1.3 7.1 - 2-oxoglutarate NAD-linked reductive amination 391618 1.4.1.3 7.1 - 2-oxoglutarate pH 8.0 655623 1.4.1.3 7.3 - L-glutamate NAD-linked oxidative deamination 391618 1.4.1.3 7.6 - NAD+ pH 9.5, 25°C, mutant enzyme E279R 656087 1.4.1.3 7.99 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K94Y 654905 1.4.1.3 8.35 - NAD+ pH 9.5, 25°C, mutant enzyme E279Y 656087 1.4.1.3 9.5 - NH3 cofactor NADPH 391623 1.4.1.3 9.98 - NAD+ pH 9.5, 25°C, mutant enzyme E279M 656087 1.4.1.3 10 - L-glutamate NADP+-dependent activity 391615 1.4.1.3 10 - NH3 in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C 684650 1.4.1.3 10.01 - NAD+ pH 9.5, 25°C, mutant enzyme E279L 656087 1.4.1.3 10.4 - NH4+ glutamate dehydrogenase 1, 10 mM 2-oxoglutarate 391607 1.4.1.3 10.41 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K118Y 654905 1.4.1.3 10.5 - 2-oxoglutarate - 391636 1.4.1.3 10.5 - 2-oxoglutarate assay at 5°C 391636 1.4.1.3 10.7 - L-glutamate wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 10.8 - L-glutamate mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 11.4 - NH3 pH 7.6, 70°C, recombinent enzyme (heated) 724160 1.4.1.3 12 - glutamate cosubstrate NAD+ 391604 1.4.1.3 12.1 - NH4+ hibernating animal, assay at 5°C 391636 1.4.1.3 12.4 - L-glutamate wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 12.8 - NH3 hGDH1, pH 8.0, temperature not specified in the publication 725866 1.4.1.3 12.9 - NH4+ glutamate dehydrogenase 2, 5 mM 2-oxoglutarate 391607 1.4.1.3 13 - NH3 pH 7.6, 70°C, native enzyme 724160 1.4.1.3 13 - NH4+ concentration range: 10-200 mM 391632 1.4.1.3 13 - NH4+ isozyme 2, amination activity, cofactor NADH 391622 1.4.1.3 13.4 - NH3 wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 14 - 2-oxoglutarate 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 14.7 - NH3 hGDH2, pH 8.0, temperature not specified in the publication 725866 1.4.1.3 15 - NH4+ cosubstrates NADPH + 2-oxoglutarate 391604 1.4.1.3 15.8 - NH4+ glutamate dehydrogenase 1, 5 mM 2-oxoglutarate 391607 1.4.1.3 15.8 - NH4+ hibernating animal, assay at 37°C 391636 1.4.1.3 17.1 - NH3 wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 17.75 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2G96Y 654905 1.4.1.3 17.8 - NH4+ - 391636 1.4.1.3 17.8 - NH4+ assay at 37°C 391636 1.4.1.3 19 - L-glutamate recombinant enzyme complex TtGDH-APRTh, high Glu concentration, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 19 - NH4+ glutamate dehydrogenase 1, 10 mM 2-oxoglutarate 391607 1.4.1.3 19.5 - L-glutamate 500 mM Tris-HCl, pH 9.4 391609 1.4.1.3 20 - NH3 kidney enzyme 391624 1.4.1.3 20.7 - glutamate mitochondrial enzyme, cofactor NADP+ 391634 1.4.1.3 21 - NH3 liver enzyme 391624 1.4.1.3 21.7 - NH4+ glutamate dehydrogenase 1, 0.1 mM NADH 391607 1.4.1.3 21.82 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K94Y 654905 1.4.1.3 22.2 - NH3 mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP 688547 1.4.1.3 22.8 - NH4+ glutamate dehydrogenase 1, 0.1 mM NADH 391607 1.4.1.3 23.8 - glutamate enzyme from rough ER, cofactor NADP+ 391634 1.4.1.3 24.7 - NH4+ euthermic animal, assay at 5°C 391636 1.4.1.3 25 - L-glutamate 0.004 mM, NAD+ 391614 1.4.1.3 25.82 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K118Y 654905 1.4.1.3 27 - NH4+ cosubstrates NADH + 2-oxoglutarate 391604 1.4.1.3 28 - NH3 cofactor NADH 391631 1.4.1.3 29 - NH3 recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication 759432 1.4.1.3 30 - NH4+ isozyme 1, amination activity, cofactor NADH 391622 1.4.1.3 30.55 - L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1G96Y 654905 1.4.1.3 31 - L-glutamate 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 33 - NH3 hGDH2, pH 7.5, temperature not specified in the publication 725866 1.4.1.3 33 - NH3 recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication 759432 1.4.1.3 35 - NH3 hGDH1, pH 7.5, temperature not specified in the publication 725866 1.4.1.3 36 - NH4+ isozyme 1, amination activity, cofactor NADPH 391622 1.4.1.3 41 - NH3 cofactor NADPH 391631 1.4.1.3 41 - NH4+ isozyme 3, amination activity, cofactor NADPH 391622 1.4.1.3 43 - NH3 recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication 759432 1.4.1.3 44 - NH3 NADPH + 2-oxoglutarate 391614 1.4.1.3 44 - NH4+ isozyme 3, amination activity, cofactor NADH 391622 1.4.1.3 46 - NH3 recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication 759432 1.4.1.3 49 - norvaline - 391612 1.4.1.3 50 - NH3 NADH + 2-oxoglutarate 391614 1.4.1.3 52 - L-glutamate recombinant enzyme complex TtGDH-APRTh, high Glu concentration, pH and temperature not specified in the publication 759432 1.4.1.3 53 - NH4+ isozyme 2, amination activity, cofactor NADPH 391622 1.4.1.3 57.5 - NH3 hGDH1, pH 7.0, temperature not specified in the publication 725866 1.4.1.3 58 - NH4+ 500 mM Tris-HCl, pH 8.2 391609 1.4.1.3 62.2 - NH3 hGDH2, pH 7.0, temperature not specified in the publication 725866 1.4.1.3 66 - NH3 NADH-dependent activity 391615 1.4.1.3 68 - NH3 pH 8.0 655623 1.4.1.3 77 - NH4+ 500 mM Tris-HCl, pH 7.2 391609 1.4.1.3 100 - NH3 biphasic Lineweaver-Burk plot suggests 2 Km values 391615 1.4.1.3 106 - NH4+ glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate 391607 1.4.1.3 115.1 - NH4+ glutamate dehydrogenase 1, 0.2 mM NADH 391607 1.4.1.3 160 - NH4+ 50 mM Tris-HCl, pH 7.2 391609