1.1.1.365 C-4 epimer galacturonate displays marked substrate inhibition 259776 1.1.1.365 glucuronate weak substrate inhibition 9173 1.1.1.365 L-gulonate competitive inhibitor, Ki in the low mM range 1757 1.1.1.365 L-gulono-1,4-lactone competitive inhibitor, Ki in the low mM range 2342 1.1.1.365 additional information substrate inhibition by aldonic acids. The products L-galactonate and L-galactono-1,4-lactone are observed to mitigate substrate inhibition by galacturonate, with the lactone having a greater effect than the acid 2 1.1.1.365 N-ethylmaleimide 84% residual activity at 1 mM 49 1.1.1.365 NADP+ mutant enzyme R267L shows a considerable sensitivity at the lower (0.16 mM) but not at the higher (0.8 mM) NADH concentration 10