3.6.1.17 additional information NSC113427, NSC133815, and NSC305522, do not exhibit any enzyme inhibition at concentrations in excess of 0.1 mM 2 3.6.1.17 ATP competitive 4 3.6.1.17 ATP - 4 3.6.1.17 ADP - 13 3.6.1.17 Zn2+ non-competitive, reversible with EDTA, very strong inhibitor 14 3.6.1.17 Zn2+ reversible with fructose-2,6-diphosphate 14 3.6.1.17 Zn2+ - 14 3.6.1.17 Ca2+ 50% inhibition at 0.025 mM 15 3.6.1.17 Ca2+ - 15 3.6.1.17 Ca2+ Ki: 0.3 mM 15 3.6.1.17 phosphate inhibitory above 30 mM 16 3.6.1.17 EDTA - 21 3.6.1.17 EDTA 5 mM, 13.2% residual activity 21 3.6.1.17 UDP - 26 3.6.1.17 AMP - 30 3.6.1.17 GTP competitive 37 3.6.1.17 GTP - 37 3.6.1.17 N-ethylmaleimide 1 mM, 73.8% residual activity 49 3.6.1.17 GDP competitive 53 3.6.1.17 GDP - 53 3.6.1.17 UTP - 65 3.6.1.17 GMP competitive 162 3.6.1.17 GMP - 162 3.6.1.17 F- - 174 3.6.1.17 F- non-competitive 174 3.6.1.17 iodoacetic acid 10 mM, 58.8% residual activity 213 3.6.1.17 fluoride - 407 3.6.1.17 fluoride inhibition at 0.003 mM F-, Ki: 0.006 mM 407 3.6.1.17 fluoride Ki: 0.05; non-competitive 407 3.6.1.17 fluoride non-competitive, strong inhibition, reversible 407 3.6.1.17 fluoride Ki: 0.02 mM 407 3.6.1.17 fluoride no effect 407 3.6.1.17 fluoride fluoride potently inhibits diadenosine tetraphosphate hydrolysis in the presence of Mg2+, whereas it is ineffective in the presence of Zn2+, because inhibition involves a specific, MgF3--containing transition state analogue complex, overview 407 3.6.1.17 p-mercuribenzoate - 686 3.6.1.17 adenosine 5'-tetraphosphate Ki in nanomolar range 1551 3.6.1.17 adenosine 5'-tetraphosphate competitive; Ki in nanomolar range 1551 3.6.1.17 adenosine 5'-tetraphosphate Ki: 0.006 mM 1551 3.6.1.17 adenosine 5'-tetraphosphate competitive 1551 3.6.1.17 guanosine 5'-tetraphosphate Ki in nanomolar range 1789 3.6.1.17 guanosine 5'-tetraphosphate Ki: 0.01 mM 1789 3.6.1.17 fructose-6-phosphate slight inhibition 4244 3.6.1.17 Ionic strength significant inhibition at high ionic strength 5668 3.6.1.17 glycerol phosphate 20 mM, 46.1% residual activity 9177 3.6.1.17 diadenosine 5',5'''-P1,P4-tetraphosphate - 9323 3.6.1.17 A-5'-p-alpha,beta,alpha',beta'-bis(oxymethylene)p-5'-A competitive inhibitor 18980 3.6.1.17 diadenosine triphosphate competitive, also phosphonate analogues of the substance 21584 3.6.1.17 1,2,3-tri(adenosine-5'-O-phosphorothio)glycerol - 22472 3.6.1.17 1,3-di(adenosine-5'-O-phospho)-2-phosphorothio-glycerol - 22473 3.6.1.17 1,4-di(adenosine-5'-O-phospho)erythritol - 22474 3.6.1.17 1,4-di(adenosine-5'-O-phosphorothio)erythritol - 22475 3.6.1.17 1,2,3,4-tetra(adenosine-5'-O-phosphorothio)erythritol - 22476 3.6.1.17 A-5'-p-beta,beta'-methyleneoxy-p-5'-A competitive inhibitor 26705 3.6.1.17 1,3-di(adenosine-5'-O-phospho)glycerol - 53849 3.6.1.17 1,3-di(adenosine-5'-O-phospho)-2-phospho-glycerol - 53850 3.6.1.17 1,2,3,4-tetra(adenosine-5'-O-phospho)erythritol - 53851 3.6.1.17 di(adenosine-5'-O-phosphorothio)-di(adenosine-5'-phospho)pentaerythritol - 53852 3.6.1.17 Ap5 - 56234 3.6.1.17 5-methyl-2-[[4-(methylamino)-9,10-dioxo-9,10-dihydroanthracen-1-yl]amino]benzenesulfonate NSC401611 81819 3.6.1.17 2-[2-[(4-aminophenyl)amino]-6-phenylpyrimidin-4-yl]phenol NSC232476 82358 3.6.1.17 2-((8-hydroxy-4-(4-methyl-2-sulfoanilino)-9,10-dioxo-9,10-dihydro-1-anthracenyl)amino)-5-methylbenzenesulfonic acid NSC51531, competitive inhibitor 83753 3.6.1.17 uridine 5'-tetraphosphate Ki in nanomolar range 107467 3.6.1.17 1,4-dihydroxy-5,8-bis[(4-methylphenyl)amino]anthracene-9,10-dione NSC86169 162280 3.6.1.17 5-hydroxy-1,4-bis[(4-methylphenyl)amino]anthracene-9,10-dione NSC300513 162294 3.6.1.17 perylene-3,4,9,10-tetracarboxylic acid NSC89768 162320 3.6.1.17 inositol 4-butyl-1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate competitive 205966 3.6.1.17 inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,4,5,6-pentaphosphate competitive 205967 3.6.1.17 inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate competitive 205968 3.6.1.17 trisodium phosphate 20 mM, 24.8% residual activity 206635