3.4.21.63 2-mercaptoethanol - 63 3.4.21.63 2-propanol inhibits at 30% v/v 682 3.4.21.63 4-nitrophenyl acetate increasing concentrations of the ester inhibit casein hydrolysis 418 3.4.21.63 acetone loss of 64-74% activity at 15-50% v/v after 24 h, loss of 27-70% activity after 1 h 333 3.4.21.63 acetone 13% inhibition at 10% 333 3.4.21.63 acetonitrile 48% inhibition at 10% 981 3.4.21.63 Ag+ - 75 3.4.21.63 Ag+ strong inhibitor of recombinant and native enzyme 75 3.4.21.63 Ag+ about 19% inhibition at 1-10 mM 75 3.4.21.63 Ag+ 72% inhibition at 1 mM 75 3.4.21.63 Ba2+ some inhibition at 10 mM 111 3.4.21.63 benzamidine slightly inhibits recombinant and native enzyme 579 3.4.21.63 benzeneboronic acid - 43808 3.4.21.63 Benzyloxycarbonyl-Ala-Gly-PheCH2Cl - 43822 3.4.21.63 butanol 50% inhibition after 2 h 626 3.4.21.63 butanol loss of 40-68% activity at 15-50% v/v after 24 h, loss of 40-47% activity after 1 h 626 3.4.21.63 Ca2+ some inhibition at 10 mM 15 3.4.21.63 Cd2+ some inhibition at 1 mM 52 3.4.21.63 cetyltrimethyl ammonium bromide - 16564 3.4.21.63 chloroform loss of 45-88.5% activity at 15-50% v/v after 24 h, loss of 31-21% activity after 1 h 4509 3.4.21.63 Co2+ some inhibition at 1 mM 23 3.4.21.63 Co2+ about 10% inhibition at 5 mM 23 3.4.21.63 Co2+ 8% inhibition at 10 mM 23 3.4.21.63 CTAB 70% inhibition at 1.0% 21954 3.4.21.63 CTAB 50% inhibition at 1.0% 21954 3.4.21.63 Cu2+ some inhibition at 1 mM 19 3.4.21.63 Cu2+ inhibitor of recombinant and native enzyme 19 3.4.21.63 Cu2+ over 90% inhibition at 5 mM 19 3.4.21.63 Cu2+ - 19 3.4.21.63 Cu2+ 7% inhibition at 1 mM 19 3.4.21.63 DFP - 923 3.4.21.63 DMSO inhibits at 30% v/v 896 3.4.21.63 DMSO loss of 41-53% activity at 15-50% v/v after 24 h, loss of 21-48% activity after 1 h 896 3.4.21.63 DTNB 52-57% inhibition at 1-5 mM 554 3.4.21.63 DTT 9.9% inhibition at 5 mM 177 3.4.21.63 DTT - 177 3.4.21.63 EDTA complete inhibition 21 3.4.21.63 EDTA slightly inhibits recombinant and native enzyme 21 3.4.21.63 EDTA - 21 3.4.21.63 EDTA 12.7% inhibition at 5 mM 21 3.4.21.63 EDTA complete inhibition at 5 mM, 50% at 1 mM 21 3.4.21.63 EDTA 15% inhibition at 10 mM 21 3.4.21.63 EGTA - 173 3.4.21.63 ethanol reversible inhibition 69 3.4.21.63 ethanol loss of 55-78% activity at 15-50% v/v after 24 h, loss of 8-55% activity after 1 h 69 3.4.21.63 ethanol 39.5% inhibition at 10% 69 3.4.21.63 Fe2+ - 25 3.4.21.63 Fe2+ 45% inhibition at 10 mM, 33.5% at 5 mM 25 3.4.21.63 Fe2+ 47% inhibition at 5 mM 25 3.4.21.63 Fe3+ - 70 3.4.21.63 Fe3+ 18.9% inhibition at 5 mM 70 3.4.21.63 formaldehyde loss of 27-13% activity at 15-50% v/v after 24 h, loss of 46-47% activity after 1 h 130 3.4.21.63 H2O2 - 22 3.4.21.63 H2O2 73-74% inhibition at 1-5 mM 22 3.4.21.63 Hg2+ some inhibition at 1 mM 33 3.4.21.63 Hg2+ - 33 3.4.21.63 Hg2+ strongly inhibits 33 3.4.21.63 Hg2+ strong inhibitor of recombinant and native enzyme 33 3.4.21.63 Hg2+ complete inhibition at 5 mM 33 3.4.21.63 Hg2+ 25.2% inhibition at 5 mM 33 3.4.21.63 Hg2+ 73% inhibition at 1 mM 33 3.4.21.63 iodoacetamide 5.2% inhibition at 5 mM 67 3.4.21.63 iodoacetic acid strong inhibition at 5 mM 213 3.4.21.63 Isopropanol inhibits at 30% v/v 958 3.4.21.63 Isopropanol loss of 39-45% activity at 15-50% v/v after 24 h, loss of about 50% activity after 1 h 958 3.4.21.63 Isopropanol 68% inhibition at 10% 958 3.4.21.63 leupeptin 68% inhibition of AP30 at 0.05 mM 217 3.4.21.63 leupeptin 48% inhibition at 10 mM 217 3.4.21.63 methanol inhibition of esterolytic activity 83 3.4.21.63 methanol loss of 75-100% activity at 15-50% v/v after 24 h, loss of 44-93% activity after 1 h 83 3.4.21.63 Mn2+ slightly inhibits 11 3.4.21.63 Mn2+ inhibitor of recombinant and native enzyme 11 3.4.21.63 Mn2+ about 30% inhibition at 5 mM 11 3.4.21.63 Mn2+ 14.1% inhibition at 5 mM 11 3.4.21.63 Mn2+ 15% inhibition at 1 mM 11 3.4.21.63 additional information cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB 2 3.4.21.63 additional information metal chelating agents; monoiodoacetic acid; not: sulfhydryl reagents; PCMB 2 3.4.21.63 additional information EDTA 2 3.4.21.63 additional information not inhibitory: commercial detergents such as Vim and Avis 2 3.4.21.63 additional information not inhibited by Cu2+, Sn2+, Ba2+, Co2+, Li+, benzamidine, beta-ME, cysteine, EDTA and 1,10-phenathroline 2 3.4.21.63 additional information poor inhibition by Ca2+ at 5 mM 2 3.4.21.63 additional information no effect by H2O2 and NaClO 2 3.4.21.63 additional information the enzyme is completely stable in presence of laundry detergents, overview 2 3.4.21.63 additional information Triton X-100 and PMSF have no inhibitory effect on the enzyme activity 2 3.4.21.63 additional information no or poor inhibition by Ba2+, Cr3+, K+, Fe3+, Ca2+, Zn2+, and Mg2+, all at 1 mM. No inhibition by methanol at 10% 2 3.4.21.63 additional information no or poor effect by 5 mM of EDTA, o-phenanthroline, bestatin, or 1 mM Zn2+ 2 3.4.21.63 additional information no inhibition by Tween 20 and Triton X-100 at 1.0% 2 3.4.21.63 additional information no inhibition by 5 mM Mg2+ 2 3.4.21.63 N-bromosuccinimide - 208 3.4.21.63 N-bromosuccinimide 50.3% inhibition at 5 mM 208 3.4.21.63 n-butanol inhibition of esterolytic activity 629 3.4.21.63 N-Chlorosuccinimide - 6001 3.4.21.63 N-diazoacetylnorleucine methyl ester 8.3% inhibition at 5 mM 239617 3.4.21.63 N-ethylmaleimide 11.2% inhibition at 5 mM 49 3.4.21.63 n-Propanol inhibition of esterolytic activity 947 3.4.21.63 N-tosyl-L-lysine chloromethyl ketone inhibitor of recombinant and native enzyme 8472 3.4.21.63 Na+ 7.5% inhibition at 5 mM 59 3.4.21.63 NaCl 18% 42 3.4.21.63 NaN3 slightly inhibits 238 3.4.21.63 Ni2+ some inhibition at 1 mM 38 3.4.21.63 Ni2+ about 20% inhibition at 5 mM 38 3.4.21.63 Ni2+ 32% inhibition at 10 mM, 28% at 5 mM 38 3.4.21.63 Pb2+ some inhibition at 10 mM 139 3.4.21.63 pepstatin 4.8% inhibition at 1 mM 396 3.4.21.63 Phenylmethyl sulfonylfluoride complete inhibition 20461 3.4.21.63 phenylmethylsulfonyl fluoride 100% inhibition of AP30 at 5 mM 257 3.4.21.63 phenylmethylsulfonyl fluoride completely inhibits 257 3.4.21.63 phenylmethylsulfonyl fluoride strong inhibitor of recombinant and native enzyme 257 3.4.21.63 PMSF - 248 3.4.21.63 PMSF 83.7% inhibition at 5 mM 248 3.4.21.63 PMSF strong inhibition at 5 mM 248 3.4.21.63 PMSF complete inhibition at 5 mM, 97% at 1 mM 248 3.4.21.63 PMSF complete inhibition 248 3.4.21.63 PMSF 92% inhibition at 1 mM 248 3.4.21.63 Potato inhibitor - 20967 3.4.21.63 SDS strong inhibition at 0.5% 124 3.4.21.63 SDS - 124 3.4.21.63 SDS complete inhibition at 5 mM, 96% at 1 mM 124 3.4.21.63 SDS complete inhibition at 1.0% 124 3.4.21.63 SDS 70% inhibition at 1.0% 124 3.4.21.63 SDS 60% inhibition at 0.5 mM 124 3.4.21.63 Sodium azide 22.0% inhibition at 5 mM 447 3.4.21.63 sodium lauryl sulfate - 3231 3.4.21.63 Sr2+ some inhibition at 10 mM 338 3.4.21.63 TLCK moderately inhibits 4621 3.4.21.63 TLCK 57% inhibition at 5 mM 4621 3.4.21.63 tosyl-L-lysylchloromethyl ketone 6.7% inhibition at 5 mM 239618 3.4.21.63 TPCK moderately inhibits 7403 3.4.21.63 Triton X-100 45% inhibition at 5 mM, 39% at 1 mM 61 3.4.21.63 Triton X-100 10% inhibition at 1.0% 61 3.4.21.63 Trypsin inhibitor moderately inhibits 2103 3.4.21.63 Tween 20 66% inhibition at 5 mM, 39% at 1 mM 555 3.4.21.63 Tween 20 5% inhibition at 1.0% 555 3.4.21.63 Tween 80 72% inhibition at 1-5 mM 514 3.4.21.63 Urea 4.5% inhibition at 5 mM 116 3.4.21.63 Urea slight inhibition at 5 mM 116 3.4.21.63 Urea 71% inhibition at 1 mM, 95.5% at 8 mM 116 3.4.21.63 Zn2+ some inhibition at 10 mM 14 3.4.21.63 Zn2+ inhibitor of recombinant and native enzyme 14 3.4.21.63 Zn2+ about 20% inhibition at 5 mM 14 3.4.21.63 Zn2+ 10.9% inhibition at 5 mM 14 3.4.21.63 Zn2+ 40% inhibition at 10 mM, 38% at 1-5 mM 14 3.4.21.63 Zn2+ 64% inhibition at 5 mM 14