3.1.30.1	1,10-phenanthroline	0.24 mM, complete inhibition of plasmid nicking activity	62	
3.1.30.1	1-ethyl-3-(3-dimethylaminopropyl)carbodiimide	inactivation	3174	
3.1.30.1	2-mercaptoethanol	with 4 mM 50% of activity with denaturated DNA	63	
3.1.30.1	2-mercaptoethanol	-	63	
3.1.30.1	2-mercaptoethanol	with 1 mM stabilization of activity at pH 3.5 and 4.0	63	
3.1.30.1	5'-ADP	-	2539	
3.1.30.1	5'-AMP	-	236	
3.1.30.1	5'-ATP	50% inhibition by 0.5 mM	3789	
3.1.30.1	5'-ATP	-	3789	
3.1.30.1	5'-dADP	-	102263	
3.1.30.1	5'-dAMP	50% inhibition by 0.085 mM dAMP	1745	
3.1.30.1	5'-dAMP	-	1745	
3.1.30.1	5'-dATP	50% inhibition by 0.001 mM dATP	16322	
3.1.30.1	5'-dATP	-	16322	
3.1.30.1	5'-GMP	-	1022	
3.1.30.1	8-hydroxychinoline 5-sulfonate	10% of maximal activity with 0.01 mM, no activity with 0.1 mM	13048	
3.1.30.1	8-hydroxychinoline 5-sulfonate	-	13048	
3.1.30.1	8-hydroxychinoline 5-sulfonate	8% of maximal activity with 0.01 mM, no activity with 0.1 mM	13048	
3.1.30.1	8-hydroxychinoline 5-sulfonate	30% of maximal activity with 0.01 mM	13048	
3.1.30.1	8-hydroxychinoline 5-sulfonate	50% of activity towards ssDNA with 0.005 mM	13048	
3.1.30.1	8-hydroxychinoline 5-sulfonate	with 1 mM 50% of maximal activity on denaturated DNA, 70% with native DNA	13048	
3.1.30.1	Ag2+	-	1041	
3.1.30.1	Al3+	-	264	
3.1.30.1	amino group modification	at pH 8.0, 65-70% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of amino groups, lysine residues, with 0.5 mM 2,4,6-trinitrobenzenesulfonic acid	47811	
3.1.30.1	amino group modification	reaction of lysine residues with phthalaldehyde, loss of 80% of initial activity with 0.1 mM	47811	
3.1.30.1	aurintricarboxylic acid	complete inhibition at 0.005 mM	1818	
3.1.30.1	beta-mercaptoethanol	-	773	
3.1.30.1	Ca2+	-	15	
3.1.30.1	Ca2+	about 40% inhibition of hydrolysis of RNA	15	
3.1.30.1	Ca2+	30% of activity towards denaturated ssDNA with 1.2 mM	15	
3.1.30.1	Ca2+	35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition	15	
3.1.30.1	Ca2+	about 10% of activity towards ssDNA at 1 and 10 mM	15	
3.1.30.1	Ca2+	1 mM, slight inhibition	15	
3.1.30.1	Ca2+	10 mM, complete inhibition of junction cleavage	15	
3.1.30.1	carboxylate group modification	at pH 4.6, 50-85% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 5-15 mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide; at pH 7.8, 55-95% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 2-15 mM Woodward's reagent K	47846	
3.1.30.1	citraconylation	reversible block of amino groups, lysine residues, by citraconic anhydride	102623	
3.1.30.1	citrate	at 0.2 M 50% of initial activity with ssDNA	131	
3.1.30.1	Co2+	almost complete inhibition of 3'-AMP hydrolysis by 1 mM CoCl2	23	
3.1.30.1	CsCl	optimal activity 0.5-1.8 M, 27% of maximal activity at 7 M	3807	
3.1.30.1	Cu2+	46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition	19	
3.1.30.1	Cu2+	1 mM, slight inhibition	19	
3.1.30.1	diethyldicarbonate	inactivation	310	
3.1.30.1	diphosphate	-	17	
3.1.30.1	dithiothreitol	-	45	
3.1.30.1	EDTA	69% of maximum activity at 20 mM	21	
3.1.30.1	EDTA	-	21	
3.1.30.1	EDTA	95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+	21	
3.1.30.1	EDTA	no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine	21	
3.1.30.1	EDTA	complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+	21	
3.1.30.1	EDTA	95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+; complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+	21	
3.1.30.1	EDTA	complete inactivation by incubation with 20 mM, pH 5.0	21	
3.1.30.1	EDTA	complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+; no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine	21	
3.1.30.1	EDTA	almost complete inactivation by 0.01 mM	21	
3.1.30.1	EDTA	15% of maximal activity with 0.01 mM	21	
3.1.30.1	EDTA	55-100% loss of initial activity with 1-10 mM	21	
3.1.30.1	EDTA	50% of activity towards ssDNA with 0.0003 mM	21	
3.1.30.1	EDTA	almost no activity with 2 mM	21	
3.1.30.1	EDTA	1 mM, slight inhibition	21	
3.1.30.1	EDTA	reversible by Zn2+	21	
3.1.30.1	EDTA	almost complete inhibition of 3'-AMP hydrolysis with 1 mM	21	
3.1.30.1	EGTA	1 mM, slight inhibition	173	
3.1.30.1	F-	complete inhibition by 10 mM	174	
3.1.30.1	F-	18% of maximal activity with 10 mM	174	
3.1.30.1	F-	5% of maximal activity with 10 mM	174	
3.1.30.1	F-	at 0.2 M 30% of initial activity with ssDNA	174	
3.1.30.1	Fe2+	-	25	
3.1.30.1	Fe2+	about 40% inhibition of hydrolysis of RNA	25	
3.1.30.1	Fe2+	47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition	25	
3.1.30.1	Fe3+	-	70	
3.1.30.1	glutathione	with 4 mM of the reduced form 50% of activity with denaturated DNA	44	
3.1.30.1	heparin	-	227	
3.1.30.1	Hg2+	complete inactivation by 5 mM HgCl2	33	
3.1.30.1	Hg2+	31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition	33	
3.1.30.1	Hg2+	-	33	
3.1.30.1	Hg2+	complete inhibition of 3'-AMP hydrolysis by 1 mM HgCl2	33	
3.1.30.1	iodoacetamide	-	67	
3.1.30.1	Ionic strength	-	5668	
3.1.30.1	Ionic strength	optimal 0.025-0.05 M	5668	
3.1.30.1	Ionic strength	dependence of tRNA hydrolysis on salt concentration	5668	
3.1.30.1	Ionic strength	inhibition of activity at ionic strength above 0.3 M	5668	
3.1.30.1	Ionic strength	about 40% of activity towards ssDNA at 0.2 M NaCl and KCl	5668	
3.1.30.1	KCl	50% of activity with 0.019 and 0.025 mM	79	
3.1.30.1	KCl	-	79	
3.1.30.1	KH2PO4	-	2966	
3.1.30.1	Mg2+	-	6	
3.1.30.1	Mg2+	about 40% inhibition of hydrolysis of RNA with 10 mM Mg2+	6	
3.1.30.1	Mg2+	concentrations higher 2 mM	6	
3.1.30.1	Mg2+	reduction of activity towards ssDNA at 10 mM	6	
3.1.30.1	Mg2+	1 mM, slight inhibition	6	
3.1.30.1	MgCl2	50 mM, 25% inhibition	196	
3.1.30.1	Mn2+	6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition	11	
3.1.30.1	additional information	urea does not influence the enzyme at any concentrations	2	
3.1.30.1	N-bromosuccinimide	DNA and RNA protects from inactivation	208	
3.1.30.1	N-ethylmaleimide	with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid	49	
3.1.30.1	NaCl	inhibition at concentrations higher than 200 mM	42	
3.1.30.1	NaCl	optimal activity at 100 mM, 55% of maximal rate at 400 mM	42	
3.1.30.1	NaCl	-	42	
3.1.30.1	NaCl	maximal activity 0-2 M, 40% of maximal activity at 4,4 M	42	
3.1.30.1	NaCl	maximal activity at 0-100 mM, at 1 mM 71% of maximal activity	42	
3.1.30.1	NaCl	complete inhibition with 50 mM	42	
3.1.30.1	NaCl	inhibition by low concentrations, 50% of activity with 19 and 0.025 mM	42	
3.1.30.1	NaCl	inhibition at concentrations higher than 100 mM	42	
3.1.30.1	NaCl	dependence of cleavage of superhelical, nicked-circular and linear DNA molecules from the NaCl concentration	42	
3.1.30.1	NaCl	inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+	42	
3.1.30.1	NaCl	strong inhibition above 100 mM	42	
3.1.30.1	NaCl	100 mM, 20% inhibition	42	
3.1.30.1	phosphate	50% inhibition by 2 mM sodium phosphate	16	
3.1.30.1	phosphate	-	16	
3.1.30.1	phosphate	80% inhibition by 33 mM potassium phosphate, pH 7.5	16	
3.1.30.1	phosphate	competitive inhibition	16	
3.1.30.1	phosphate	45% of maximal activity with 20 mM potassium phosphate, 26% with 30 mM	16	
3.1.30.1	Polyvinyl sulfate	-	15927	
3.1.30.1	potassium citrate	complete inhibition at 10 mM	44720	
3.1.30.1	reductive methylation	modification of lysine residues into N,N'-dimethyl lysine	103587	
3.1.30.1	SDS	-	124	
3.1.30.1	sodium bisphosphate	50% inhibition by 0.02 mM sodium bisphosphate	48161	
3.1.30.1	sodium bisphosphate	at 0.2 M 10% of initial activity with ssDNA	48161	
3.1.30.1	sodium dodecylsulfate	above 0.04% decrease of reaction rate. 60% of activity at 0.12%	1066	
3.1.30.1	sodium dodecylsulfate	a third of maximal activity at 0.1%, but slight activation below 0.01%	1066	
3.1.30.1	sodium dodecylsulfate	up to 0.6% no effect on DNA hydrolysis	1066	
3.1.30.1	sodium dodecylsulfate	-	1066	
3.1.30.1	sodium dodecylsulfate	complete inactivation with 0.01% at pH 5.0	1066	
3.1.30.1	sodium dodecylsulfate	complete inhibition with 0.2%	1066	
3.1.30.1	Urea	40% of maximal activity with single-stranded DNA with 6.6 M	116	
3.1.30.1	Zn2+	54% of initial activity towards ssDNA with 0.1 mM Zn2+	14	
3.1.30.1	Zn2+	54% of initial activity towards ssDNA with 0.1 mM Zn2+; almost complete inactivation at 1.0 mM	14	
3.1.30.1	Zn2+	33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition	14	
3.1.30.1	Zn2+	about 15% of activity towards ssDNA at 1 and 10 mM	14	
3.1.30.1	Zn2+	-	14