3.1.1.75 1,2-epoxy-3-(p-nitrophenoxy)propane complete inactivation at 100 mM, slow inactivation process 2924 3.1.1.75 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/glycinamide 33% inhibition at 5 mM 196863 3.1.1.75 2-Hydroxy-5-nitrobenzyl bromide complete inactivation 3778 3.1.1.75 2-mercaptoethanol strong inhibition at 4 mM 63 3.1.1.75 2-mercaptoethanol 1% (v/v): 20% activity, compared to no addition 63 3.1.1.75 2-mercaptoethanol - 63 3.1.1.75 2-propanol partial inhibition of P(3HV) depolymerase 682 3.1.1.75 2-propanol 71% inhibition at 10% (vol/vol) 682 3.1.1.75 3-hydroxybutyrate cyclic trimers, reversible inhibition 3600 3.1.1.75 acetone 82% inhibition at 10% (vol/vol) 333 3.1.1.75 acetonitrile 70% inhibition at 10% (vol/vol) 981 3.1.1.75 Ag+ marked inhibition at 0.1 mM 75 3.1.1.75 Ag+ 10 mM, 38% inhibition 75 3.1.1.75 Aprotinin 10 mM, 26% inhibition 405 3.1.1.75 Ba(CH3COO)2 complete inhibition at 10 mM 43804 3.1.1.75 Ba2+ 5 mM, 32% loss of activity 111 3.1.1.75 Ca2+ 5 mM, 44% loss of activity 15 3.1.1.75 Ca2+ - 15 3.1.1.75 Ca2+ activates up to 10 mM, inhibits above; activates up to 10 mM, inhibits above 15 3.1.1.75 CaCl2 at 10 mM 39% inhibition 218 3.1.1.75 citrate depressed activity 131 3.1.1.75 Cu2+ 5 mM, complete loss of activity 19 3.1.1.75 Cu2+ - 19 3.1.1.75 Cu2+ 1 mM, 41% inhibition 19 3.1.1.75 Cu2+ 1 mM, 30% residual activity 19 3.1.1.75 diazo-DL-norleucine methyl ester complete inhibition at 25 mM, pseudo-first-order kinetic 32201 3.1.1.75 diazo-DL-norleucine methyl ester very sensitive to 32201 3.1.1.75 diazo-DL-norleucine methyl ester 97% inhibition at 25 mM 32201 3.1.1.75 diethyldicarbonate 40% inhibition at 5 mM, 90% at 20 mM 310 3.1.1.75 diethylene glycol 71% inhibition at 10% (vol/vol) 47197 3.1.1.75 diisopropyl fluorophosphate 90% inhibition at 10 mM 244 3.1.1.75 diisopropyl fluorophosphate complete inhibition at 500 nM 244 3.1.1.75 diisopropyl fluorophosphate very sensitive to 244 3.1.1.75 diisopropyl fluorophosphate - 244 3.1.1.75 diisopropylfluorophosphate almost complete inhibition at 1 mM 299 3.1.1.75 diisopropylfluorophosphate at 10 microM 53% inhibition, at 100 microM total loss of activity 299 3.1.1.75 dithioerythritol complete inhibition of P(3HV) depolymerase at 4 mM, reversible by dithionitrobenzene 577 3.1.1.75 dithiothreitol 83% inhibition at 10 mM 45 3.1.1.75 dithiothreitol complete inhibition at 5 mM, reversible by H2O2 45 3.1.1.75 dithiothreitol - 45 3.1.1.75 dithiothreitol rapid inactivation 45 3.1.1.75 dithiothreitol considerable inactivation 45 3.1.1.75 dithiothreitol strong inhibition at 4 mM 45 3.1.1.75 dithiothreitol at 0.1 mM 86% inhibition, complete inhibition at 1 mM 45 3.1.1.75 dithiothreitol complete inhibition at 5 mM 45 3.1.1.75 dithiothreitol 70% inhibition at 1 mM and 90% inhibition at 10 mM 45 3.1.1.75 dithiothreitol inactivation of the purified enzyme 45 3.1.1.75 dithiothreitol 1 mM, complete inhibition 45 3.1.1.75 dithiothreitol 5 mM, complete inhibition 45 3.1.1.75 DMF 84% inhibition at 10% (vol/vol) 59318 3.1.1.75 DMSO 85% inhibition at 10% (vol/vol) 896 3.1.1.75 DTE - 16666 3.1.1.75 DTT - 177 3.1.1.75 EDTA partial inhibition of P(3HV) depolymerase at concentrations higher than 5 mM 21 3.1.1.75 EDTA 79% activity at 1 mM, 20% activity at 20 mM 21 3.1.1.75 EDTA 10 mM, 27% inhibition 21 3.1.1.75 EDTA 0.5%, 83% loss of activity 21 3.1.1.75 EDTA 0.1 mM: 51%, 1 mM: 24%, 10 mM: 17% activity, compared to no addition 21 3.1.1.75 EDTA - 21 3.1.1.75 EDTA activates alone, but inhibits in presence of divalent cations; activates alone, but inhibits in presence of divalent cations 21 3.1.1.75 EDTA 1 mM, 50% residual activity 21 3.1.1.75 ethanol partial inhibition of P(3HV) depolymerase 69 3.1.1.75 ethanol 82% inhibition at 10% (vol/vol) 69 3.1.1.75 ethylene glycol 28% inhibition at 10% (vol/vol) 1410 3.1.1.75 Fe2+ 56% inhibition at 1 mM, almost complete inhibition at 4 mM 25 3.1.1.75 Fe2+ 10 mM, 17% inhibition 25 3.1.1.75 Fe2+ 5 mM, 50% loss of activity 25 3.1.1.75 Fe2+ - 25 3.1.1.75 Fe2+ 88% inhibition at 5 mM 25 3.1.1.75 Fe2+ 1 mM, 84% inhibition 25 3.1.1.75 Fe2+ 1 mM, 90% residual activity 25 3.1.1.75 Fe2+ 1 mM less than 10% residual activity 25 3.1.1.75 Fe3+ 5 mM, 38% loss of activity 70 3.1.1.75 H2O2 - 22 3.1.1.75 Hg2+ marked inhibition at 0.1 mM 33 3.1.1.75 Hg2+ 90% inhibition at 6 mM, completely reversible by 2-mercaptoethanol 33 3.1.1.75 Hg2+ 5 mM, 97% loss of activity 33 3.1.1.75 Hg2+ complete inhibition at 5 mM 33 3.1.1.75 Hg2+ 1 mM, 82% inhibition 33 3.1.1.75 iodoacetamide - 67 3.1.1.75 K+ 5 mM, 32% loss of activity 39 3.1.1.75 K+ - 39 3.1.1.75 KCl 100 mM, 75% inhibition 79 3.1.1.75 mercaptoethanol 0.5%, 47% loss of activity 1623 3.1.1.75 Mercuric chloride complete inhibition at 10 mM 5577 3.1.1.75 methanol 91% inhibition at 10% (vol/vol) 83 3.1.1.75 Mg2+ - 6 3.1.1.75 Mg2+ activates up to 10 mM, inhibits above; activates up to 10 mM, inhibits above 6 3.1.1.75 MgCl2 slight inhibition 1 mM, 57% inhibition at 10 mM 196 3.1.1.75 Mn2+ 10 mM, 17% inhibition 11 3.1.1.75 Mn2+ - 11 3.1.1.75 additional information no inhibition by phenylmethylsulfonyl fluoride 2 3.1.1.75 additional information no inhibition of activity by azide 2 3.1.1.75 additional information activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenyl butyrate. Poor effect by DTT; activation of PhaZd2 depolymerase activity by salts, but salts affect the activity of PhaZd2 with nPHB granules as substrate but have no significant influence on the hydrolysis of p-nitrophenyl esters such as p-nitrophenylbutyrate. Poor effect by DTT 2 3.1.1.75 additional information no or poor inhibition by EDTA, Triton X-10, Tween-20, and urea; no or poor inhibition by Triton X-10, Tween-20, and urea 2 3.1.1.75 additional information poor inhibition by glycerol. Increase of ionic strength gradually inhibits the poly(3-hydroxybutyrate) depolymerase activity leading to its complete inactivation at 2.5 M NaCl 2 3.1.1.75 N-bromosuccinimide complete inactivation 208 3.1.1.75 N-bromosuccinimide inactivation of the purified enzyme 208 3.1.1.75 N-ethylmaleimide 1 mM, complete inhibition 49 3.1.1.75 Na+ 5 mM, 10% loss of activity 59 3.1.1.75 Na+ - 59 3.1.1.75 NaCl 1 mM: 100%, 2 mM: 90%, 5 mM: 60%, 10 mM: 45% activity, compared to no addition 42 3.1.1.75 NaCl complete inhibition at 3 M 42 3.1.1.75 NaCl complete inactivation at 2.5 M 42 3.1.1.75 NaCl required for native enzyme, optimum activity at 0.5 M. Inhibitory for recombinant enzyme 42 3.1.1.75 NaN3 1 mM: 86%, 10 mM: 84% activity, compared to no addition 238 3.1.1.75 Ni2+ 10 mM, 28% inhibition 38 3.1.1.75 p-(chloromercuri)benzoic acid no inhibition below 1 mM 129641 3.1.1.75 p-chloromercuribenzoic acid complete inhibition at 0.1 mM 614 3.1.1.75 Pb2+ marked inhibition at 0.1 mM 139 3.1.1.75 phenylmethanesulfonyl fluoride considerable inactivation 827 3.1.1.75 phenylmethanesulfonyl fluoride 42% inhibition at 10 mM 827 3.1.1.75 Phenylmethylsulfonylfluoride 10% inhibition at 1 mM and 70% inhibition at 10 mM 1148 3.1.1.75 Plysurf A210G 100 mg/l, 48% inhibition 138737 3.1.1.75 PMSF marked inhibition at 0.1 mM 248 3.1.1.75 PMSF 49% inhibition at 10 mM 248 3.1.1.75 PMSF complete inactivation at 1 mM 248 3.1.1.75 PMSF partial inhibition at 0.5 mM 248 3.1.1.75 PMSF 10 mM, 72% inhibition 248 3.1.1.75 PMSF 1 mM: 24%, 10 mM: 10% activity, compared to no addition 248 3.1.1.75 PMSF - 248 3.1.1.75 PMSF 5 mM, complete inhibition 248 3.1.1.75 SDS almost complete inhibition at 0.1% 124 3.1.1.75 SDS at 0.01% (w/v) 28% inhibition, at 0.1% complete inhibition 124 3.1.1.75 SDS almost complete inhibition at 0.01% v/v; almost complete inhibition at 0.01% v/v 124 3.1.1.75 SDS 10 mM, 86% inhibition 124 3.1.1.75 SDS 0.1%, complete inhibition 124 3.1.1.75 tetrahydrofuran 51% inhibition at 10% (vol/vol) 2760 3.1.1.75 triethylene glycol 73% inhibition at 10% (vol/vol) 196862 3.1.1.75 Triton X-100 competitive inhibition, Ki: 0.014% 61 3.1.1.75 Triton X-100 - 61 3.1.1.75 Triton X-100 complete inhibition at at 0.1% (w/v) 61 3.1.1.75 Triton X-100 almost complete inhibition at 0.1% 61 3.1.1.75 Triton X-100 at 0.1% 57% inhibition, at 1% 64% inhibition 61 3.1.1.75 Triton X-100 0.1% (v/v): 115%, 1% (v/v): 70%, 10% (v/v): 56% activity, compared to no addition 61 3.1.1.75 Triton X-100 almost complete inhibition at 0.01% v/v; almost complete inhibition at 0.01% v/v 61 3.1.1.75 Tween 20 nearly complete inhibition at 0.1%, v/v 555 3.1.1.75 Tween 20 considerable inactivation 555 3.1.1.75 Tween 20 almost complete inhibition at 0.1% 555 3.1.1.75 Tween 20 amlnost complete inhibition at 0.1% 555 3.1.1.75 Tween 20 0.1% (v/v): 100%, 1% (v/v): 64%, 10% (v/v): 25% activity, compared to no addition 555 3.1.1.75 Tween 20 - 555 3.1.1.75 Tween 20 0.1%, complete inhibition 555 3.1.1.75 Tween 80 competitive inhibition, Ki: 0.56% 514 3.1.1.75 Tween 80 almost complete inhibition at 0.1% 514 3.1.1.75 Tween 80 0.1% (v/v): 80%, 1% (v/v): 65%, 10% (v/v): 42% activity, compared to no addition 514 3.1.1.75 Tween 80 - 514 3.1.1.75 tyrosine depressed activity 709 3.1.1.75 Zn2+ 5 mM, 88% loss of activity 14 3.1.1.75 Zn2+ - 14