2.7.1.78 (NH4)2SO4 - 399 2.7.1.78 (NH4)2SO4 strong inhibition 399 2.7.1.78 2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione A12B4C3 5856 2.7.1.78 2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione A12B4C3, noncompetitive inhibition 5856 2.7.1.78 2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione A6B4C3 39792 2.7.1.78 2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione A1B4C3 77449 2.7.1.78 2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione A39B1C2 77450 2.7.1.78 5'-AMP weak 236 2.7.1.78 5'-Hydroxyl poly(I) in combination with 5'-hydroxyl poly(A) or poly(C) 95973 2.7.1.78 ADP - 13 2.7.1.78 ADP complete inhibition at 1 mM 13 2.7.1.78 ADP strong inhibition 13 2.7.1.78 AgNO3 - 360 2.7.1.78 AgNO3 complete inhibition at 0.3 mM 360 2.7.1.78 ammonium sulfate about 75% inhibition at 10 mM 1058 2.7.1.78 ammonium sulfate 80% inhibition at 30 mM 1058 2.7.1.78 ammonium sulfate 50% inhibition at 8.5 mM 1058 2.7.1.78 ammonium sulfate - 1058 2.7.1.78 beta,gamma-imidoadenosine 5'-triphosphate binds with high affinity, similar to ATP 57032 2.7.1.78 beta,gamma-imidoadenylyl 5'-tetraphosphate ATP analog, competitive against ATP, noncompetitive against 5'-OH-DNA, serves as substrate for the reverse reaction only 22092 2.7.1.78 Ca2+ inhibits in combination with MgCl2, stimulates without MgCl2 15 2.7.1.78 Cd2+ - 52 2.7.1.78 chloramphenicol inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol 955 2.7.1.78 Cibacron blue F3GA chromophore of blue dextran, inhibition is competitive to single stranded DNA, noncompetitive with respect to ATP 4231 2.7.1.78 CTP more than 90% inhibition at 0.3 mM 60 2.7.1.78 Cu2+ inhibits in presence of CuCl2 19 2.7.1.78 Cu2+ inhibits in presence of MgCl2 19 2.7.1.78 Cu2+ - 19 2.7.1.78 dATP more than 95% inhibition at 0.3 mM 140 2.7.1.78 dCTP 80% inhibition at 0.3 mM 179 2.7.1.78 Deoxyribonucleoside triphosphates - 96385 2.7.1.78 Dextran sulfate forward and reverse reaction at similar amounts; strong inhibition, competitive to ATP and DNA 2775 2.7.1.78 Dextran sulfate strong inhibition 2775 2.7.1.78 Dextran sulfate - 2775 2.7.1.78 dGTP more than 95% inhibition at 0.3 mM 219 2.7.1.78 diphosphate - 17 2.7.1.78 diphosphate weak 17 2.7.1.78 diphosphate 97% inhibition at 20 mM 17 2.7.1.78 diphosphate 50% inhibition at 2.2 mM 17 2.7.1.78 disodium hydrogen phosphate - 213355 2.7.1.78 dTTP more than 95% inhibition at 0.3 mM 145 2.7.1.78 EDTA complete inhibition at 25 mM 21 2.7.1.78 EDTA - 21 2.7.1.78 GTP more than 90% inhibition at 0.3 mM 37 2.7.1.78 heparin - 227 2.7.1.78 heparin strong inhibition 227 2.7.1.78 iodoacetate - 93 2.7.1.78 iodoacetate complete inhibition at 0.3 mM 93 2.7.1.78 KCl - 79 2.7.1.78 KCl KCl stimulates at low concentrations, inhibits at high concentrations 79 2.7.1.78 KCl weak inhibition, 50% at about 0.3 M 79 2.7.1.78 KCl 50% inhibition at 71 mM 79 2.7.1.78 KCl inhibitory above 10 mM 79 2.7.1.78 Maleate - 575 2.7.1.78 Mg2+ 1-2 mM, maximum activity, inhibitory above 6 2.7.1.78 Mn2+ inhibition above 1 mM 11 2.7.1.78 Mn2+ maximum activity at 0.01 mM, inhibitory above 0.1 mM 11 2.7.1.78 additional information no inhibition by chondroitin sulfates A and C, and by dextran 2 2.7.1.78 additional information no inhibition by agar-agar 2 2.7.1.78 additional information productive engagement of a 3'-phosphate terminus may block access of a 5'-hydroxyl to the kinase active site 2 2.7.1.78 N-ethylmaleimide - 49 2.7.1.78 Na2HPO4 - 3652 2.7.1.78 NaCl - 42 2.7.1.78 NaCl stimulates activity towards single stranded substrates, inhibitory with some duplexes 42 2.7.1.78 NaCl maximum activity in presence of 0.1-0.15 M NaCl, higher concentrations inhibit 42 2.7.1.78 NaCl weak inhibition, 50% at about 0.3 M 42 2.7.1.78 NaCl 50% inhibition at 67 mM 42 2.7.1.78 NaCl inhibitory above 10 mM 42 2.7.1.78 NH4+ - 54 2.7.1.78 NH4+ weak inhibition 54 2.7.1.78 NH4+ 50% at about 0.3 M; weak inhibition 54 2.7.1.78 Ni2+ inhibits in combination with MgCl2, stimulates without MgCl2 38 2.7.1.78 p-chloromercuribenzoate reversible by 2-mercaptoethanol 43 2.7.1.78 p-chloromercuribenzoate reversible 43 2.7.1.78 p-chloromercuribenzoate - 43 2.7.1.78 p-hydroxymercuribenzoate 2-mercaptoethanol prevents inhibition 98 2.7.1.78 PEG 6000 5-15%, 3-4fold increase in activity, inhibitory above 7634 2.7.1.78 phosphate - 16 2.7.1.78 phosphate weak 16 2.7.1.78 phosphate 50% inhibition at 11 mM 16 2.7.1.78 Ribonucleoside 3'-phosphates weak 97077 2.7.1.78 Ribonucleoside triphosphates - 30894 2.7.1.78 spermine 1 mM enhances activity 3times, inhibition above 1 mM 197 2.7.1.78 sulfate - 160 2.7.1.78 sulfate weak 160 2.7.1.78 sulfate competitive to ATP, noncompetitive to DNA, less sensitive in the reverse reaction 160 2.7.1.78 sulfate 50% inhibition at 5.7 mM 160 2.7.1.78 sulfhydryl antagonists - 114464 2.7.1.78 tert-butyl 2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridine-1yl-carbamate A26B11C2 155467 2.7.1.78 UTP more than 90% inhibition at 0.3 mM 65 2.7.1.78 Zn2+ inhibition in presence of Mg2+ 14