1.4.1.9 4-methyl-2-pentanone competitive inhibition of wild-type enzyme, noncompetitive inhibition of mutant enzyme K80A 12372 1.4.1.9 4-methylpentanoate competitive inhibition of wild-type enzyme and mutant enzyme K80A 8383 1.4.1.9 4-methylpentanoate - 8383 1.4.1.9 Ag+ 1 mM, no residual activity 75 1.4.1.9 AgNO3 1 mM, complete 360 1.4.1.9 Cd2+ 1 mM, 8% residual activity 52 1.4.1.9 Co2+ CoCl2 23 1.4.1.9 Co2+ - 23 1.4.1.9 Cr2+ 1 mM, 13% residual activity 1919 1.4.1.9 Cu(CH3COO)2 1 mM, 20% inhibition 20200 1.4.1.9 Cu2+ 1 mM CuCl2, 22% inhibition 19 1.4.1.9 Cu2+ CuSO4 19 1.4.1.9 Cu2+ - 19 1.4.1.9 Cu2+ 1 mM, 58.7% residual activity 19 1.4.1.9 Cu2+ 1 mM, 61% residual activity 19 1.4.1.9 D-2-aminobutanoate - 12487 1.4.1.9 D-alloisoleucine - 43968 1.4.1.9 D-Leu - 1717 1.4.1.9 D-norleucine - 4000 1.4.1.9 D-Norvaline - 5538 1.4.1.9 D-Val - 2157 1.4.1.9 EDTA 1 mM, 92% residual activity 21 1.4.1.9 Fe2+ 1 mM, 75% residual activity 25 1.4.1.9 Fe3+ 1 mM, no residual activity 70 1.4.1.9 Hg2+ 1 mM, 29% residual activity 33 1.4.1.9 HgCl2 1 mM, 92% inhibition 110 1.4.1.9 HgCl2 0.01 M, 65% inhibition 110 1.4.1.9 HgCl2 - 110 1.4.1.9 HgCl2 1 mM, complete 110 1.4.1.9 KCl 0.5 M, specific activity 133 U/mg 79 1.4.1.9 KCN 1 mM, 43% inhibition 161 1.4.1.9 L-Leu competitive inhibition of the reductive amination of 4-methylthio-2-oxobutanoate 424 1.4.1.9 MgCl2 - 196 1.4.1.9 Mn2+ inhibits oxidative deamination 11 1.4.1.9 Mn2+ 1 mM, 32.7% residual activity 11 1.4.1.9 additional information not inhibitory: EDTA, Ca2+, Mg2+ 2 1.4.1.9 Na2S - 2087 1.4.1.9 NaCl 0.5 M, specific activity 152 U/mg 42 1.4.1.9 NAD+ product inhibition 7 1.4.1.9 NADH noncompetitive inhibition with respect to L-Leu 8 1.4.1.9 NADH competitive inhibition versus NAD+, noncompetitive inhibition with respect to L-Leu 8 1.4.1.9 NH4+ inhibits oxidative deamination 54 1.4.1.9 p-mercuribenzoate 1 mM, 75% inhibition 686 1.4.1.9 p-mercuribenzoate - 686 1.4.1.9 Pb(CH3COO)2 1 mM, 20% inhibition 2865 1.4.1.9 Pb2+ 1 mM, no residual activity 139 1.4.1.9 PCMB - 78 1.4.1.9 PCMB reversed by L-Cys 78 1.4.1.9 PCMB 0.01 M, 50% inhibition 78 1.4.1.9 pyridoxal 5'-phosphate - 32 1.4.1.9 pyridoxal 5'-phosphate fully protected from inactivation only by NADH 32 1.4.1.9 Sulfide - 318 1.4.1.9 Sulfide Na2S 318 1.4.1.9 Thiourea 1 mM, 52% residual activity 844 1.4.1.9 Zn2+ 1 mM, 80% residual activity 14