2.4.2.18 dilution inactivates 638674 2.4.2.18 freezing and thawing inactivates 638674 2.4.2.18 glycerol, 10%, is essential for storage, but it must be replaced by polyethylene glycol to achieve crystals that are not severely temperature dependent and radiation sensitive 638676 2.4.2.18 the enzyme is either digested by trypsin, V8-protease or thermolysin after incubation for 1 h at 25°C but not by thermolysin at 70°C 638678