4.2.1.32	100 mM GSH plus 1mM Fe2+ optimal reactivation of inactive enzyme	5653	
4.2.1.32	cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity	5656	
4.2.1.32	crude cell-extract completely loses activity in open tubes in ice bath for 2-4 h, activity restored after incubation at 25°C for 30 min, extent of activation decreases with each succeeding cycle of inactivation and reactivation, loss of activity after treatment with EDTA or after dialysis	5653	
4.2.1.32	Cysteine 100 mM Cysteine plus 1 mM Fe2+ optimal reactivation of inactive enzyme	5653	
4.2.1.32	equimolar mixture of 50 mM GSH and 50 mM Cysteine plus 1 mM Fe2+ optimum reactivation of inactive enzyme	5653	
4.2.1.32	manipulations on dye-ligand columns cause rapid irrecoverable loss of activity	5654	
4.2.1.32	no significant loss of activity after repeated freezing and thawing	5651	
4.2.1.32	sucrose and/or succinate stabilize	5654