3.4.22.10	0.1 N HCl or 0.01 N NaOH, room temperature, 2 h, denaturation	
3.4.22.10	0.1 N HCl, 2 h, denaturation of the zymogen	
3.4.22.10	0.5% SDS, denaturation	
3.4.22.10	8 M urea plus reducing agent, 20% of proteolytic activity is provided	
3.4.22.10	EDTA, 0.001 M, stabilizes