1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus coagulans Adh is increased by the endogenous activator protein Act, a Nudix hydrolase 743223 1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, in a pH-dependent manner. Act increases the Vmax of the enzyme and decreases the Km for methanol, the increase was 23fold at pH 7.4 and 50fold at pH 9.0 742514 1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, no activation in vivo 743223 1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh3 is increased by the endogenous activator protein Act, a Nudix hydrolase 743223 1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Desulfitobacterium hafniense Adh is increased by the endogenous activator protein Act, a Nudix hydrolase 743223 1.1.1.244 Act protein activator protein, UniProt ID I3EA59, the in vitro activity of Lysinibacillus sphaericus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase 743223 1.1.1.244 Activator protein - 286376 1.1.1.244 Activator protein activation in presence of Mg2+, in vitro: 3 mol soluble activator protein per mol enzyme, in vivo estimated as 1 mol per 17.5 mol enzyme, MW 50000 with two subunits of MW 27000 each, in presence of activator protein and Mg2+ enzyme possesses a high affinity active site for alcohol and NAD+, up to 40fold increase of activity, primarily of Vmax, slight decrease of Km-value for methanol, enzyme/activator-interaction is dilution-sensitive, no stimulation of formaldehyde reduction 286375 1.1.1.244 Activator protein each activator protein subunit binds one molecule of NADH, activator may facilitate oxidation of reduced enzyme bound NADH cofactor 286377 1.1.1.244 activator protein ACT all three isozymes are activated by ACT, which is encoded by gene act, overview 726329