3.4.22.33	2-mercaptoethanol	highest activity at 96 mM, at 37°C and pH 7.0	682716	
3.4.22.33	dithiothreitol	highest activity at 2.5 mM, at pH 7.0 and 37°C	682716	
3.4.22.33	EDTA	28% increase of activity at 2 mM	682716	
3.4.22.33	EDTA	activates about 2.5fold	753642	
3.4.22.33	additional information	high-pressure treatment of fruit bromelain with 300 MPa, at 20°C for 20 min: high pressure processing (HPP) affects the fibrinolytic activity of fruit bromelain in vivo, overview. Compared with untreated fruit bromelain, the fibrinolytic activity of high-pressure processing-treated fruit bromelain is obviously increased which might be attributed to the increase of alpha-helix and the exposure of tryptophan residues and tyrosine residues, overview. High-pressure processing-treated fruit bromelain shows an increase (7.67%) in alpha-helix and its fluorescence intensities are increased by 42.5% and 78.7% at the excitation wavelength of 295 nm and 280 nm, respectively	754304	
3.4.22.33	additional information	no effect on activity by EDTA	753642	
3.4.22.33	Urea	activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate	682716