5.3.99.3 17beta-estradiol up-regulates PTGES mRNA and protein level 692090 5.3.99.3 A23187 - 660973 5.3.99.3 all-trans retinoic acid ATRA 693774 5.3.99.3 angiotensin II angiotension II infusion induces mRNA expression of mPGES-1, mPGES-2 and cytosolic PGE synthase 692588 5.3.99.3 bradykinin - 660973 5.3.99.3 captoprylsulfobetaine 10 mM, 2.6fold activation 653753 5.3.99.3 diheptanoylphosphatidylcholine 2.2 mM, 4.8fold activation 653753 5.3.99.3 dihydrolipoic acid - 690714 5.3.99.3 dithiothreitol - 690714 5.3.99.3 dithiothreitol activates 3127 5.3.99.3 dithiothreitol leads to dissociation of the bound heme which is hindered by imidazole 727942 5.3.99.3 epigallocatechin gallate enhances COX-2 and isoform mPGES-1 gene expression and production of prostaglandin E2. Induction of mPGES-1 is mediated by active ERK1/2MAP kinases and early growth response gene 1 678094 5.3.99.3 glutathione - 660669, 660677, 662071, 662768, 663277, 690714, 748542 5.3.99.3 glutathione the N-terminal domain contains the GSH-binding site (G-site) 726884 5.3.99.3 GSH 2 mM required for maximal activity of IgG1(hei-7)-reactive protein 3126 5.3.99.3 GSH activates 3127 5.3.99.3 GSH concentrations below 0.5 mM are required for maximal activity of IgG1(hei-26)-reactive protein 3126 5.3.99.3 GSH dependent on 3126, 3128, 3129 5.3.99.3 GSH mPGES2 has a GSH-binding motif, the GSH molecule binds at the predicted amino acid residues 148VPxL to 164DSxxI. GSH is essential for heme binding to isozyme mPGES2, structures of GSH-heme complex-binding cavity in isozyme mPGES2, overview 727942 5.3.99.3 GSH the enzyme activity is increased in the presence of reduced glutathione 747606 5.3.99.3 GSH two different types of prostaglandin E synthase: 1. GSH-dependent enzyme, 2. GSH-independent enzyme. The genitally organs, especially the deferent duct have mainly GSH-dependent prostaglandin E synthase activity. Heart, spleen and uterus have only the GSH-independent activity. In absence of reduced glutathione the enzyme from deferent duct shows only 3% of the activity in presence of reduced glutathione 3134 5.3.99.3 Hsp90 maximal activity is achieved when the three components (cPGES, casein kinase II and Hsp90) are mixed at a molar proportion of 1:1:1 660973 5.3.99.3 interleukin 1beta increase in protein expression and activity of isoform mPGES-1, accompanied by increased COX-2 expression and production of prostaglandin E2 678444 5.3.99.3 interleukin 1beta induces isoform mPGES-1 and subsequent production of prostaglandin E2, while production of prostaglandin I2 is reduced by increased expression of mPGES-1 681664 5.3.99.3 interleukin 1beta stimulates release of prostaglandin E2 by myometrical smooth muscle cells and increases COX-2 and mPGES-1 mRNA and protein expression 681963 5.3.99.3 interleukin-1beta - 692090, 693551, 693823 5.3.99.3 lipopolysaccharide lipopolysaccharide binds to receptor TLR4 on osteoblasts that directly induces isoform mPGES-1 expression for prostaglandin E2 synthesis, leading to subsequent bone resorption 681197 5.3.99.3 LPS - 693551 5.3.99.3 additional information IL-1 administration into the mouse cortex leads to elevated expression of cPGES and an increase in prostaglandin E2 662071 5.3.99.3 additional information lipopolysaccharide significantly increases the mRNA level of mPGES-1 690374 5.3.99.3 additional information mPGES-1 expression is upregulated in COX-1 and COX-2 knock-out cells 694941 5.3.99.3 additional information phosphorylation is crucial for the enzyme's function, phosphorylation by casein kinase II enhances cPGES activity 3fold 660973 5.3.99.3 additional information proinflammatory cytokinin, induction of the enzyme 662768 5.3.99.3 additional information proinflammatory stimuli 662071 5.3.99.3 n-dodecylmaltoside 0.3 mM, 5fold activation 653753 5.3.99.3 n-octylglucoside 34 mM, 3.3fold activation 653753 5.3.99.3 thiol reagents - 662071 5.3.99.3 TNF-alpha - 693452 5.3.99.3 TNF-beta - 693452 5.3.99.3 TNFalpha - 692090 5.3.99.3 Triton X-100 5 mM, 4.6fold activation 653753 5.3.99.3 Tumor necrosis factor alpha increase in protein expression and activity of isoform mPGES-1, accompanied by increased COX-2 expression and production of prostaglandin E2 678444