3.4.22.49	cell division cycle 6	Cdc6, a mitotic substrate of polo-like kinase. Cleavage of chesin/Rad21 is much greater in wild-type Cdc6 expressing cells than in GFP and Cdc6-T37V mutant expressing cells	718320	
3.4.22.49	cyclinB1	-	732703	
3.4.22.49	DNA	chromosomal DNA is required as a cofactor for the cleavage of cohesin to occur, and allows separase to selectively cleave only the chromosome-associated cohesin	718177	
3.4.22.49	DNA	chromosomal DNA is required as a cofactor for the cleavage of cohesin to occur, and allows separase to selectively cleave only the chromosome-associated cohesin. Separase binds to DNA in a sequence nonspecific manner in vitro and associates with the entire length of the mitotic chromosomes	718177	
3.4.22.49	imatinib	activation of separase proteolytic activity occurs exclusively in BCR-ABL-positive cells during imatinib treatment (0.00025-0.01 mM for 24 h)	732703	
3.4.22.49	additional information	Cdc20 is essential for mitotic progression	718037	
3.4.22.49	additional information	most of the budding yeast cohesin is cleaved in anaphase, and this cleavage is stimulated by phosphorylation of the Scc1 subunit by the Plk1 kinase	718177	
3.4.22.49	additional information	separase can be activated by incubating immunoglobulin G-Sepharose 6 containing ZZ TEV4-separase with anaphase-initiated Xenopus cytostatic factor to degrade its inhibitor securin at room temperature for 1 h	707105	
3.4.22.49	additional information	separase shows self-cleavage upon activation	664553	
3.4.22.49	PP2A	-	732703	
3.4.22.49	securin	-	638862	
3.4.22.49	securin	activation may be due to separase localization	638862	
3.4.22.49	securin	securin is required to support separase activity in anaphase	638864	
3.4.22.49	securin	the central domain of securin has a functionally essential specific sequence that may directly interact with the catalytic region of separase. This central securin domain is unrelated to destruction by polyubiquitination, but essential for the activation of separase	665049	
3.4.22.49	securing	-	732703	
3.4.22.49	securing	the motif containing H134 in securing isoform PTTG1 has a strong affinity for separase and is involved in inhibiting it, while another domain(s) in isoform PTTG2 is involved in activating separase and has a weaker affinity for it. Isoform PTTG2 does not interact with separase	732505