3.4.22.10 dithiothreitol reduction is essential for catalytic activity 3.4.22.10 additional information incubation with human alveolar basal epithelial A549 cells induce enzyme expression 3.4.22.10 additional information serine protease high-temperature requirement A plays an indirect role in the maturation process of SpeB 3.4.22.10 Reducing agent once the enzyme is activated by a reducing agent, the presence of the activator is no longer necessary for the catalytic activity 3.4.22.10 thioethanol reduction is essential for catalytic activity, once the enzyme is activated by the reducing agent, the presence of the activator is no longer necessary for catalytic activity 3.4.22.10 thioglycolic acid reduction is essential for catalytic activity