3.4.19.3 1,10-phenanthroline weak activation 95283 3.4.19.3 cyclosporin increases particulate and soluble PAP activity in the renal cortex. Particulate PAP activity returns to levels similar to controls after 15 days of cyclosporin withdrawal, soluble PAP does not regress. Soluble and particulate aminopeptidases in resident and elicited macrophages are unaffected by cyclosporin 680172 3.4.19.3 DFP weak activation 95283 3.4.19.3 dithiothreitol - 678405, 682806 3.4.19.3 dithiothreitol absolute requirement for maintenance of activity 95301 3.4.19.3 dithiothreitol at least 10 mM required for full activity 681817 3.4.19.3 dithiothreitol presence of disulfide-reducing agent such as dithiothreitol required 95296 3.4.19.3 dithiothreitol rapid increase in activity from 2 to 6 mM, above this concentration plateau effect 681896 3.4.19.3 dithiothreitol strongly stimulates activity of wild-type enzyme and mutant enzyme C188S and shifts the pH-optimum to higher temperatures by about 10°C 651099 3.4.19.3 DTT enhances activity 653129 3.4.19.3 EDTA stimulates 95298 3.4.19.3 EDTA weak activation 95283 3.4.19.3 glucose alters the hypothalamic activity of soluble PAP-I 682252 3.4.19.3 Insulin alters the hypothalamic activity of soluble PAP-I 682252 3.4.19.3 methanol a methanol based deblocking solution preserves enzymatic activity, provides conditions compatible with sequencing and enhances deblocking of electroblotted samples 678405 3.4.19.3 PMSF weak activation 95283 3.4.19.3 thio-reducing agent absolute requirement 653759 3.4.19.3 tris(2-carboxyethyl)phosphine at least 0.5 mM required for full activity 681817