2.3.1.135 cellular retinol-binding protein CRBP, required for enzyme activity, CRBP-I and CRBP-III each compensate for the absence of the other, specifically in mammary tissue, adipose tissue, muscle, and heart 674449 2.3.1.135 cellular retinol-binding protein i.e. CRALBP, in presence of palmitoyl-CoA and CRALBP, Muller cell membranes synthesize 11-cis-retinyl ester from 11-cis-retinol at a rate which is 20fold higher than that of all-trans-retinyl ester, in absence of CRALBP, 11-cis-retinyl ester synthesis is greatly reduced by 7fold 672075 2.3.1.135 additional information activation and mRNA expression for LRAT is blocked completely in vivo by actinomycin D 638614, 638624 2.3.1.135 additional information activation is blocked completely in vivo by cycloheximide 638614 2.3.1.135 additional information retinoic acid must be continuously present to maintain LRAT gene expression in the ON position 638624 2.3.1.135 additional information the pollutant 2,3,7,8-tetrachlorodibenzo-4-dioxin, i.e. TCDD, induces enzyme expression in the kidney, induction mechanism, the expression of the cellular retinol binding protein I is unaffected, TCDD alters the retinoid metabolism regulation, expression and activity of cytochrome P450 A1 increases retinoic acid levels in kidney and liver, and enhances enzyme expression in the kidney 658432 2.3.1.135 N-(4-hydroxyphenyl)-retinamide necessary dose, 0.5 mg 638616 2.3.1.135 N-(4-hydroxyphenyl)-retinamide restores normal level of LRAT activity in vitamin A depleted rats 638616 2.3.1.135 retinoic acid - 638619 2.3.1.135 retinoic acid minimal dose for significant increase in LRAT activity and mRNA expression: 0.005 mg 638624 2.3.1.135 retinoic acid optimal dose, 0.020 mg 638614 2.3.1.135 retinoic acid restores normal level of liver LRAT activity in vitamin A deficient and depleted rats 638614 2.3.1.135 retinol - 638616 2.3.1.135 SDS SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability 735707 2.3.1.135 Vitamin A - 638614, 638616 2.3.1.135 Vitamin A regulates LRAT activity in the liver but not in the small intestine 638619