1.13.11.34 (13S)-hydroperoxy-9,11-octadecadienoic acid - 687264 1.13.11.34 (13S)-hydroperoxy-9,11-octadecadienoyl lysophosphatidylcholine 50% activation concentration AC50 is 0.0015 mM 687264 1.13.11.34 1,25(OH)2D3 gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells 704997 1.13.11.34 5,8,11,14,17-eicosapentaenoic acid competitive inhibition of oxygenation of arachidonic acid 439466 1.13.11.34 5-lipoxygenase activating protein 5-lipoxygenase activating protein is crucial for conversion of endogenous substrate by 5LO 5-lipoxygenase activating protein also stimulates the utilization of exogenous arachidonic acid, and greatly (190fold) stimulates utilization of (5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid 711100 1.13.11.34 5-lipoxygenase-activating protein a 18000 Da integral membrane protein required, in peripheral cells, for the activation of 5-lipoxygenase 439477 1.13.11.34 5-lipoxygenase-activating protein functional 5-LOX requires binding to 5-lipoxygenase-activating protein which helps 5-LOX binding to arachidonic acid at the nuclear membrane and enhances the efficiency of leukotriene synthesis 711045 1.13.11.34 5-LO activating protein i.e. FLAP, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein 689872 1.13.11.34 5-LO-activating protein i.e. FLAP, a small permanently membrane-bound protein that is essential for the biosynthesis of leukotrienes from endogenous arachidonic acid. FLAP binds arachidonic acid and is thought to assist in the provision of substrate to 5-LO 690170 1.13.11.34 5-LOX activating protein - 711661 1.13.11.34 7-thiaarachidonic acid - 439460 1.13.11.34 adenosine 58% of the activation with ATP 439464 1.13.11.34 adenosine slight stimulation 439466 1.13.11.34 adenosine stimulates at 0.01 mM or lower 439445 1.13.11.34 Adenosine 5'-O-(2-thiodiphosphate) stimulates at 0.01 mM or lower 439445 1.13.11.34 ADP 74% of the activation with ATP 439464 1.13.11.34 ADP about half as active as ATP 439461 1.13.11.34 ADP stimulation 439466 1.13.11.34 ADP-beta-S 82% of the activation with ATP 439464 1.13.11.34 AMP 68% of the activation with ATP 439464 1.13.11.34 AMP about half as active as ATP 439461 1.13.11.34 AMP stimulation 439466 1.13.11.34 AMP-4-nitrophenylphosphate 77% of the activation with ATP 439464 1.13.11.34 ATP - 684399, 684672, 688512, 689872, 743614 1.13.11.34 ATP activates, hyperbolic kinetic parameters for ATP activation indicate a similar activation for arachidonate and (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate. Solvent isotope effect results for both hydroperoxidation and epoxidation indicate that a specific step in the molecular mechanism is changed, possibly because of a lowering of the dependence of the rate-limiting step on hydrogen atom abstraction and an increase in the dependency on hydrogen bond rearrangement. Changes in ATP concentration in the cell can affect the production of 5-LOX products, such as leukotrienes and lipoxins, and thus have wide implications for the regulation of cellular inflammation 741898 1.13.11.34 ATP half-maximal activity at 0.02 mM 439453 1.13.11.34 ATP half-maximal stimulation at 0.031 mM 439457 1.13.11.34 ATP required 439449, 439459 1.13.11.34 ATP stimulates 439441, 439442, 439443, 439448, 439451, 439453, 439456, 439458, 439461, 439466, 439479 1.13.11.34 ATP upregulation of 5-LO through the C2-like domain 690170 1.13.11.34 ATP-alphaS 118% of the activation with ATP 439464 1.13.11.34 ATP-gammaS 80% of the activation with ATP 439464 1.13.11.34 cAMP stimulation 439466 1.13.11.34 CLP can upregulate and modulate the 5-lipoxygenase pathway in vitro 704997 1.13.11.34 coactosin-like protein binds 5-LO and promotes leukotriene formation, coactosin-like protein prevents heat-inactivation of 5-LO 711134 1.13.11.34 coactosin-like protein functions as a 5-LO chaperone 712666 1.13.11.34 coactosin-like protein stoichiometry 1:1 676811 1.13.11.34 CTP about half as active as ATP 439461 1.13.11.34 FLAP 5-lipoxygenase-activating protein, exclusively located at the nuclear membrane 741975 1.13.11.34 FLAP 5-lipoxygenase-activating protein. Inhibitor BRP-7 blocks 5-LOX co-localization with FLAP at the nuclear envelope in neutrophils 742210 1.13.11.34 FLAP 5-LO-activating protein, a small permanently membrane-bound protein that is essential for the biosynthesis of leukotrienes from endogenous arachidonic acid. FLAP binds arachidonic acid and is thought to assist in the provision of substrate to 5-LO 690170 1.13.11.34 FLAP required for activity 743582 1.13.11.34 FLAP the 5-LOX activating protein (FLAP) assists the translocation of 5-LOX from plasma membranes to nucleus upon cell stimulation 742135 1.13.11.34 GTP stimulation 439466 1.13.11.34 Guanosine 5'-O-(2-thiodiphosphate) stimulates at 0.01 mM or lower 439445 1.13.11.34 guanosine 5'-O-(3-thiotriphosphate) 10fold stimulation of arachidonic acid oxidation at 0.0005-0.001 mM 439445 1.13.11.34 additional information Ca2+-ionophore A23187 693577 1.13.11.34 additional information calcium ionophore A23187 increases concentrations of 5(S)-hydroxy-6,8,11,14-(E,Z,Z,Z)-eicosatetraenoic acid from 0.07 to 45.5 pmol/10000000 cells 704492 1.13.11.34 additional information coactosin-like protein, i.e. CLP, can bind to 5-LO and supports Ca2+-induced 5-LO enzyme activity. CLP seems to function as a chaperone or scaffold for 5-LO, upregulation of 5-LO through the C2-like domain 690170 1.13.11.34 additional information coactosin-like protein, i.e. CLP, can bind to 5-LO and supports Ca2+-induced 5-LO enzyme activity. CLP seems to function as a chaperone or scaffold for 5-LO. About 100fold induction of 5-LO mRNA, protein and activity is found after differentiation of HL-60 and human monocytic Mono Mac 6 cells with TGF-beta and 1,25(OH)2D3, upregulation of 5-LO through the C2-like domain. Factors like cell stress and phorbol ester activate MAPK kinases, which phosphorylate the enzyme and enhance its activity and induce nuclear translocation, overview 690170 1.13.11.34 additional information in the mouse 5-lipoxygenase gene there are no GC-boxes in tandem, the core promoter contains only one Sp1/3 binding site 704997 1.13.11.34 additional information stimulated with Ca-ionophore A 23187 705045 1.13.11.34 additional information the enzym eis upregulated in Epstein-Barr virus infected cells 685926 1.13.11.34 additional information the enzyme mutant 5-LODELTA4 stimulates wild-type 5-LO activity and product formation at low protein concentrations 743614 1.13.11.34 Nicotine treatment with 0.01 mM nicotine activates 5-lipoxygenase gastric cancer cell lines from 36-72 h 711661 1.13.11.34 phosphatidyl choline addition increases catalytic activity of wild-type nzyme by 50% 726804 1.13.11.34 phosphatidylcholine - 711134, 712666 1.13.11.34 phosphatidylcholine enzyme activity is supported by phosphatidylcholine more than by any other lipid tested, except for a cationic lipid, which is more stimulatory than phosphatidylcholine 658043 1.13.11.34 phosphatidylcholine stimulates 439442, 439443 1.13.11.34 phosphocholine phosphocholine in combination with Ca2+ markedly stimulate the formation of leukotrienes by wild-type 5-LO and mutant C159S/C300S/C416S/C418S, whereas their effect on the 5-LO W13A/W75A/W102A mutant is small 741975 1.13.11.34 protein factors from human leukocyte characterization of the membrane-associated stimulating factor 439459 1.13.11.34 protein factors from human leukocyte stimulates 439438, 439448 1.13.11.34 transforming growth factor beta gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells 704997 1.13.11.34 UTP stimulation 439466