Catalysis of the reaction: ATP + biotin + protein = AMP + diphosphate + biotin-protein
1. GOC: mah
2. EC 6.3.43. MetaCyc BIOTINLIG-RXN4. Reactome: R-HSA-2993447 "HLCS biotinylates 6x(PCCA:PCCB)"
5. Reactome: R-HSA-2993799 "HLCS biotinylates 6xMCCC1:6xMCCC2"
6. Reactome: R-HSA-2993802 "HLCS biotinylates PC:Mn2+"
7. Reactome: R-HSA-2993814 "HLCS biotinylates ACACA:Mn2+"
8. Reactome: R-HSA-3323184 "Defective HLCS does not biotinylate ACACA:Mn2+"
9. Reactome: R-HSA-4167511 "HLCS biotinylates ACACB"
10. Reactome: R-HSA-9035987 "Defective HLCS does not biotinylate 6xMCCC1:6xMCCC2"
11. Reactome: R-HSA-9035988 "Defective HLCS does not biotinylate PC:Mn2+"
12. Reactome: R-HSA-9035990 "Defective HLCS does not biotinylate 6x(PCCA:PCCB)"