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Literature summary extracted from

  • Miyatake, K.; Nakano, Y.; Kitaoka, S.
    Pantothenate synthetase from Escherichia coli [D-pantoate:beta-alanine ligase (AMP-forming), EC 6.3.2.1] (1979), Methods Enzymol., 62, 215-219.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.1 lyophilized enzyme is very stable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.1 1,10-phenanthroline
-
Escherichia coli
6.3.2.1 4-Amino-3-hydroxybutanoate 32% inhibition at 1 mM Escherichia coli
6.3.2.1 4-aminobutanoate 27% inhibition at 1 mM Escherichia coli
6.3.2.1 Ag+
-
Escherichia coli
6.3.2.1 aspartate 24% inhibition at 1 mM Escherichia coli
6.3.2.1 Ba2+
-
Escherichia coli
6.3.2.1 Ca2+
-
Escherichia coli
6.3.2.1 Co2+
-
Escherichia coli
6.3.2.1 Cu2+
-
Escherichia coli
6.3.2.1 EDTA
-
Escherichia coli
6.3.2.1 EGTA
-
Escherichia coli
6.3.2.1 Fe2+ slight Escherichia coli
6.3.2.1 gluconate 32% inhibition at 1 mM Escherichia coli
6.3.2.1 glycolate 30% inhibition at 1 mM Escherichia coli
6.3.2.1 Hg2+
-
Escherichia coli
6.3.2.1 methylmalonate 38% inhibition at 1 mM Escherichia coli
6.3.2.1 Mg2+ inhibition above 10 mM Escherichia coli
6.3.2.1 Mn2+ inhibition above 5 mM Escherichia coli
6.3.2.1 Ni2+ slight Escherichia coli
6.3.2.1 Pb2+ slight Escherichia coli
6.3.2.1 Sn2+ slight Escherichia coli
6.3.2.1 Sr2+ slight Escherichia coli
6.3.2.1 taurine 52% inhibition at 1 mM Escherichia coli
6.3.2.1 Zn2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.1 0.063
-
D-pantoate
-
Escherichia coli
6.3.2.1 0.1
-
ATP
-
Escherichia coli
6.3.2.1 0.15
-
beta-Alanine
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.1 K+ activates Escherichia coli
6.3.2.1 K+ Km: 5.5 mM Escherichia coli
6.3.2.1 Mg2+ divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above Escherichia coli
6.3.2.1 Mn2+ divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above Escherichia coli
6.3.2.1 NH4+ activates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.1 18000
-
x * 18000, SDS-PAGE in presence of 2-mercaptoethanol Escherichia coli
6.3.2.1 69000
-
gel filtration Escherichia coli
6.3.2.1 70000
-
sedimentation equilibrium ultracentrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Escherichia coli
-
IFO 13168
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.1 2.05
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.3.2.1 -20°C, stable for 6 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
Escherichia coli AMP + diphosphate + (R)-pantothenate
-
?

Subunits

EC Number Subunits Comment Organism
6.3.2.1 ? x * 18000, SDS-PAGE in presence of 2-mercaptoethanol Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.1 37
-
pH 5.0-10.0, 15 min stable Escherichia coli