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Literature summary extracted from

  • Blumberg, S.; Schechter, I.; Berger, A.
    The purification of papain by affinity chromatography (1970), Eur. J. Biochem., 15, 97-102.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 HgCl2
-
 Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 18
-
 Nalpha-benzoyl-L-Arg ethyl ester
-
 Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.2
-
 Carica papaya

Storage Stability

EC Number Storage Stability Organism
3.4.22.2 4°C, protein concentration 0.04 mM, 2 mM EDTA, pH 4.3, 3% loss of activity after 48 h, 10% loss of activity after 10 days Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 additional information existence of an acyl-enzyme intermediate Carica papaya ?
-
 ?
3.4.22.2 N-acetyl-L-Trp p-nitrophenyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 N-benzyloxycarbonyl-L-Lys + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
 Carica papaya ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 28.5
-
 Nalpha-benzoyl-L-Arg ethyl ester
-
 Carica papaya