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Literature summary extracted from

  • Quong, M.W.; Miyada, C.G.; Switchenko, A.C.; Goodman, T.C.
    Identification, purification, and characterization of a D-arabinitol-specific dehydrogenase from Candida tropicalis (1993), Biochem. Biophys. Res. Commun., 196, 1323-1329.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.250 analysis the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis Candida tropicalis

General Stability

EC Number General Stability Organism
1.1.1.11 Mg2+ stabilizes Candida tropicalis
1.1.1.250 Mg2+ enhances the stability Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.11 0.12
-
 NAD+
-
 Candida tropicalis
1.1.1.11 39.8
-
 D-arabinitol
-
 Candida tropicalis
1.1.1.250 0.12
-
 NAD+
-
 Candida tropicalis
1.1.1.250 39.8
-
 D-arabinitol
-
 Candida tropicalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.11 Mg2+ 1-5 mM increases activity about 50%, also enhances stability Candida tropicalis
1.1.1.250 Mg2+ 1-5 mM, 50% increase of activity Candida tropicalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.11 31000
-
 x * 31000, SDS-PAGE Candida tropicalis
1.1.1.250 31000
-
 x * 31000, SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.11 D-arabinitol + NAD+ Candida tropicalis
-
 D-ribulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.11 Candida tropicalis
-
-
-
1.1.1.250 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.250
-
 Candida tropicalis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.250 D-arabinitol + NAD+ = D-ribulose + NADH + H+ in the reverse reaction the enzyme catalyzes transfer of 4(S) hydrogen of NADH Candida tropicalis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.11 96
-
-
 Candida tropicalis
1.1.1.250 96
-
-
 Candida tropicalis

Storage Stability

EC Number Storage Stability Organism
1.1.1.250 -80°C, stable Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.11 D-arabinitol + NAD+
-
 Candida tropicalis D-ribulose + NADH + H+
-
 ?
1.1.1.11 Galactitol + NAD+ 1.6% activity Candida tropicalis ?
-
 ?
1.1.1.11 L-Xylulose + NADH 3% activity Candida tropicalis ?
-
 ?
1.1.1.11 additional information no activity with D-mannitol Candida tropicalis ?
-
 ?
1.1.1.11 Xylitol + NAD+ 5% activity Candida tropicalis ?
-
 ?
1.1.1.250 D-arabinitol + NAD+ r Candida tropicalis D-ribulose + NADH + H+
-
 ?
1.1.1.250 D-xylulose + NADH 1% of the activity with D-ribulose Candida tropicalis ?
-
 ?
1.1.1.250 Galactitol + NAD+ 1.6% of the activity with D-arabinitol Candida tropicalis ?
-
 ?
1.1.1.250 L-Xylulose + NADH 3% of the activity with D-ribulose Candida tropicalis ?
-
 ?
1.1.1.250 Xylitol + NAD+ 5% of the activity with D-arabinitol Candida tropicalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.11 ? x * 31000, SDS-PAGE Candida tropicalis
1.1.1.250 ? x * 31000, SDS-PAGE Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.11 6.5
-
 optimal activity, reduction Candida tropicalis
1.1.1.250 10 11 oxidation of D-arabinitol Candida tropicalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.11 10 11 enzyme activity increases with increasing pH, measurements above pH 11.0 not feasible Candida tropicalis
1.1.1.250 8 11 pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity Candida tropicalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.11 6 9.5 more labile at increasing pH from 6.0-9.5 Candida tropicalis
1.1.1.250 11
-
 unstable above Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.11 NAD+
-
 Candida tropicalis
1.1.1.11 NADH
-
 Candida tropicalis
1.1.1.250 NAD+
-
 Candida tropicalis
1.1.1.250 NADH
-
 Candida tropicalis