EC Number | General Stability | Organism |
---|---|---|
3.4.21.25 | 20% dimethyl sulfoxide solution, 20°C, stable | Cucumis melo |
3.4.21.25 | 8 M urea, pH 9.1, 20 min, no loss of activity | Cucumis melo |
3.4.21.25 | alpha-chymotrypsin has no effect on the activity | Cucumis melo |
3.4.21.25 | incubation with trypsin for 4 h decreases the activity to 30% of the initial activity and after 24 h the enzyme is completely inactivated | Cucumis melo |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.25 | 2.02 | - |
Benzyloxycarbonyl-Glu-Tyr | - |
Cucumis melo | |
3.4.21.25 | 3.33 | - |
benzoyl-Tyr-ethyl ester | - |
Cucumis melo |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 67000 | - |
SDS-PAGE | Cucumis melo |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.25 | Protein + H2O | Cucumis melo | - |
? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.21.25 | 2-propanol | 20% w/v, at 37°C for 1 h, no effect | Cucumis melo |
3.4.21.25 | acetonitrile | 20% w/v, at 37°C for 1 h, no effect | Cucumis melo |
3.4.21.25 | dioxan | 20% w/v, at 37°C for 1 h, no effect | Cucumis melo |
3.4.21.25 | Ethanol | 20% w/v, at 37°C for 1 h, no effect | Cucumis melo |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.25 | Cucumis melo | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.25 | 40fold | Cucumis melo |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.25 | acetyl-Ala-Ala-Ala-p-nitroanilide + H2O | 1% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester | Cucumis melo | ? | - |
? | |
3.4.21.25 | benzoyl-Tyr-ethyl ester + H2O | 24% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester | Cucumis melo | benzoyl-Tyr + ethanol | - |
? | |
3.4.21.25 | Benzyloxycarbonyl-Glu-Tyr + H2O | - |
Cucumis melo | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
3.4.21.25 | benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O | best synthetic substrate | Cucumis melo | benzyloxycarbonyl-Tyr + 4-nitrophenyl ester | - |
? | |
3.4.21.25 | casein + H2O | - |
Cucumis melo | ? | - |
? | |
3.4.21.25 | Insulin B-chain + H2O | broad substrate specificity, but the bonds of the valine residue are not split | Cucumis melo | ? | - |
? | |
3.4.21.25 | additional information | no hydrolysis of Lys-p-nitroanilide, Tyr-p-nitroanilide, Leu-p-nitroanilide, tosyl-Arg-methyl ester, acetyl-Phe-ethyl ester, benzyloxycarbonyl-Lys-nitrophenyl ester and benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide | Cucumis melo | ? | - |
? | |
3.4.21.25 | Protein + H2O | - |
Cucumis melo | ? | - |
? | |
3.4.21.25 | protein + H2O | - |
Cucumis melo | hydrolyzed protein | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 25 | - |
20 h at a pH of 11, 80% of the activity | Cucumis melo |
3.4.21.25 | 37 | - |
24 h, 80% of the activity remains | Cucumis melo |
3.4.21.25 | 47 | - |
20 min at a pH of 11, no loss of activity | Cucumis melo |
3.4.21.25 | 60 | - |
1 h at a pH of 11, 65% of the activity | Cucumis melo |
3.4.21.25 | 72 | - |
inactivated after 20 min, ethylene glycol does not stablize, glycerol is effective to some extent | Cucumis melo |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.25 | 0.22 | - |
benzoyl-Tyr-ethyl ester | - |
Cucumis melo | |
3.4.21.25 | 2.2 | - |
benzyloxycarbonyl-Tyr 4-nitrophenyl ester | - |
Cucumis melo |