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Literature summary extracted from

  • Kaneda, M.; Yonezawa, H.; Uchikoba, T.
    Improved isolation, stability and substrate specificity of cucumisin, a plant serine endopeptidase (1995), Biotechnol. Appl. Biochem., 22, 215-222.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.25 20% dimethyl sulfoxide solution, 20°C, stable Cucumis melo
3.4.21.25 8 M urea, pH 9.1, 20 min, no loss of activity Cucumis melo
3.4.21.25 alpha-chymotrypsin has no effect on the activity Cucumis melo
3.4.21.25 incubation with trypsin for 4 h decreases the activity to 30% of the initial activity and after 24 h the enzyme is completely inactivated Cucumis melo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.25 2.02
-
Benzyloxycarbonyl-Glu-Tyr
-
Cucumis melo
3.4.21.25 3.33
-
benzoyl-Tyr-ethyl ester
-
Cucumis melo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.25 67000
-
SDS-PAGE Cucumis melo

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.25 Protein + H2O Cucumis melo
-
?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.25 2-propanol 20% w/v, at 37°C for 1 h, no effect Cucumis melo
3.4.21.25 acetonitrile 20% w/v, at 37°C for 1 h, no effect Cucumis melo
3.4.21.25 dioxan 20% w/v, at 37°C for 1 h, no effect Cucumis melo
3.4.21.25 Ethanol 20% w/v, at 37°C for 1 h, no effect Cucumis melo

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.25 Cucumis melo
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.25 40fold Cucumis melo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.25 acetyl-Ala-Ala-Ala-p-nitroanilide + H2O 1% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester Cucumis melo ?
-
?
3.4.21.25 benzoyl-Tyr-ethyl ester + H2O 24% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester Cucumis melo benzoyl-Tyr + ethanol
-
?
3.4.21.25 Benzyloxycarbonyl-Glu-Tyr + H2O
-
Cucumis melo Benzyloxycarbonyl-Glu + Tyr
-
?
3.4.21.25 benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O best synthetic substrate Cucumis melo benzyloxycarbonyl-Tyr + 4-nitrophenyl ester
-
?
3.4.21.25 casein + H2O
-
Cucumis melo ?
-
?
3.4.21.25 Insulin B-chain + H2O broad substrate specificity, but the bonds of the valine residue are not split Cucumis melo ?
-
?
3.4.21.25 additional information no hydrolysis of Lys-p-nitroanilide, Tyr-p-nitroanilide, Leu-p-nitroanilide, tosyl-Arg-methyl ester, acetyl-Phe-ethyl ester, benzyloxycarbonyl-Lys-nitrophenyl ester and benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide Cucumis melo ?
-
?
3.4.21.25 Protein + H2O
-
Cucumis melo ?
-
?
3.4.21.25 protein + H2O
-
Cucumis melo hydrolyzed protein
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.25 25
-
20 h at a pH of 11, 80% of the activity Cucumis melo
3.4.21.25 37
-
24 h, 80% of the activity remains Cucumis melo
3.4.21.25 47
-
20 min at a pH of 11, no loss of activity Cucumis melo
3.4.21.25 60
-
1 h at a pH of 11, 65% of the activity Cucumis melo
3.4.21.25 72
-
inactivated after 20 min, ethylene glycol does not stablize, glycerol is effective to some extent Cucumis melo

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.25 0.22
-
benzoyl-Tyr-ethyl ester
-
Cucumis melo
3.4.21.25 2.2
-
benzyloxycarbonyl-Tyr 4-nitrophenyl ester
-
Cucumis melo