Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamagata, H.; Masuzawa, T.; Nagaoka, Y.; Ohnishi, T.; Iwasaki, T.
    Cucumisin, a serine protease from melon fruits, shares structural homology with subtilisin and is generated from a large precursor (1994), J. Biol. Chem., 269, 32725-32731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.25 cloned in Escherichia coli Y1090r- Cucumis melo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.25 54000
-
native enzyme, a large precursor has a MW of 67000 Da, consists of a 14000 Da C-terminal polypeptide and the active 54000 Da protease domain, which arises by limited autolysis, SDS-PAGE Cucumis melo

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.25 Protein + H2O Cucumis melo
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.25 Cucumis melo
-
L. var reticulatus cv. Teresa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.25
-
Cucumis melo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.25 fruit
-
Cucumis melo
-
3.4.21.25 juice
-
Cucumis melo
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.25 Protein + H2O
-
Cucumis melo ?
-
?
3.4.21.25 protein + H2O
-
Cucumis melo hydrolyzed protein
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.25 More
-
-