Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Awade, A.; Gonzales, T.; Cleuziat, P.; Baudouy, J.R.
    One step purification and characterization of the pyrrolidone carboxyl peptidase of Streptococcus pyogenes over-expressed in Escherichia coli (1992), FEBS Lett., 308, 70-74.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.3 overexpression in Escherichia coli Streptococcus pyogenes

General Stability

EC Number General Stability Organism
3.4.19.3 freezing and thawing, 67% of the activity is recovered after one cycle, 70% loss of activity after 5 cycles Streptococcus pyogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 2-Pyrrolidone competitive Streptococcus pyogenes
3.4.19.3 Co2+
-
Streptococcus pyogenes
3.4.19.3 Hg2+
-
Streptococcus pyogenes
3.4.19.3 iodoacetamide
-
Streptococcus pyogenes
3.4.19.3 iodoacetate
-
Streptococcus pyogenes
3.4.19.3 Zn2+
-
Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.3 1.79
-
Pyroglutamyl-beta-naphthylamide
-
Streptococcus pyogenes
3.4.19.3 20
-
pyroglutamyl-p-nitroanilide
-
Streptococcus pyogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.3 23500
-
4 * 23500, SDS-PAGE Streptococcus pyogenes
3.4.19.3 85000
-
nondenaturing PAGE Streptococcus pyogenes

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Streptococcus pyogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3
-
Streptococcus pyogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.3 20.8
-
-
Streptococcus pyogenes

Storage Stability

EC Number Storage Stability Organism
3.4.19.3 4°C, almost 60% of the initial activity is recovered after 1 month Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 pyroglutamyl-beta-naphthylamide + H2O
-
Streptococcus pyogenes pyroglutamate + beta-naphthylamine
-
?
3.4.19.3 pyroglutamyl-p-nitroanilide + H2O
-
Streptococcus pyogenes pyroglutamate + p-nitroaniline
-
?

Subunits

EC Number Subunits Comment Organism
3.4.19.3 tetramer 4 * 23500, SDS-PAGE Streptococcus pyogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.3 22
-
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month Streptococcus pyogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.3 7
-
-
Streptococcus pyogenes