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Literature summary extracted from

  • Tsuru, D.; Fujiwara, K.; Kado, K.
    Purification and characterization of L-pyrrolidonecarboxylate peptidase from Bacillus amyloliquefaciens (1978), J. Biochem., 84, 467-476.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.19.3 2-mercaptoethanol and EDTA stabilize Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 Cd2+ CdCl2 Bacillus amyloliquefaciens
3.4.19.3 Cu2+ CuCl2 Bacillus amyloliquefaciens
3.4.19.3 DFP
-
 Bacillus amyloliquefaciens
3.4.19.3 Hg2+ HgCl2 Bacillus amyloliquefaciens
3.4.19.3 L-pyroglutamate weak Bacillus amyloliquefaciens
3.4.19.3 N-bromosuccinimide
-
 Bacillus amyloliquefaciens
3.4.19.3 Ni2+ NiSO4, weak Bacillus amyloliquefaciens
3.4.19.3 PCMB
-
 Bacillus amyloliquefaciens
3.4.19.3 PCMB
-
 Niallia circulans
3.4.19.3 Sodium tetrathionate
-
 Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.19.3 CaCl2 1 mM, weak activation Bacillus amyloliquefaciens
3.4.19.3 FeCl2 1 mM, weak activation Bacillus amyloliquefaciens
3.4.19.3 FeCl3 1 mM, weak activation Bacillus amyloliquefaciens
3.4.19.3 MgCl2 1 mM, weak activation Bacillus amyloliquefaciens
3.4.19.3 MnCl2 1 mM, weak activation Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.3 24000
-
 3 * 24000, SDS-PAGE Bacillus amyloliquefaciens
3.4.19.3 72000
-
 gel filtration Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Bacillus amyloliquefaciens
-
-
-
3.4.19.3 Bacillus subtilis
-
 strain PCI 219, ML-208 and strain DT-39
-
3.4.19.3 Bacillus subtilis
-
 strain mito, strain NSI and strain tamarii
-
3.4.19.3 Micrococcus luteus
-
-
-
3.4.19.3 Niallia circulans
-
-
-
3.4.19.3 Paenibacillus polymyxa
-
-
-
3.4.19.3 Pimelobacter simplex
-
-
-
3.4.19.3 Priestia megaterium
-
-
-
3.4.19.3 Proteus vulgaris
-
-
-
3.4.19.3 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3
-
 Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.3 additional information
-
-
 Bacillus amyloliquefaciens

Storage Stability

EC Number Storage Stability Organism
3.4.19.3 -20°C, pH 7, in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol, stable for 3 months Bacillus amyloliquefaciens
3.4.19.3 4°C, pH 7, in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol, less than 50% loss of activity Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 pyroglutamyl-4-methylumbelliferyl ester + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.4.19.3 pyroglutamyl-Ala + H2O
-
 Bacillus amyloliquefaciens pyroglutamate + Ala
-
 ?
3.4.19.3 pyroglutamyl-beta-naphthylamide + H2O
-
 Bacillus subtilis pyroglutamate + beta-naphthylamine
-
 ?
3.4.19.3 pyroglutamyl-D-Ala + H2O
-
 Bacillus amyloliquefaciens pyroglutamate + D-Ala
-
 ?
3.4.19.3 pyroglutamyl-D-Leu + H2O
-
 Bacillus amyloliquefaciens pyroglutamate + D-Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.3 trimer 3 * 24000, SDS-PAGE Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.3 50
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.3 40
-
 pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.3 7 8
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.19.3 5.5 10 pH 5.5: about 50% of maximal activity, pH 10.0: about 65% of maximal activity Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.19.3 7 8 30°C, stable in presence of 5 mM EDTA and 2-mercaptoethanol Bacillus amyloliquefaciens