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Literature summary extracted from

  • Mitta, M.; Miyagi, M.; Kato, I.; Tsunasawa, S.
    Identification of the catalytic triad residues of porcine liver acylamino acid-releasing enzyme (1998), J. Biochem., 123, 924-931.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.1 expression of wild-type and mutant enzymes in Escherichia coli Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.19.1 A587S no hydrolytic activity Sus scrofa
3.4.19.1 N675S no hydrolytic activity Sus scrofa
3.4.19.1 Y707H no hydrolytic activity Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.1 0.49
-
 N-acetyl-Met-Ala wild-type enzyme Sus scrofa
3.4.19.1 0.56
-
 N-acetyl-Met-Ala recombinant wild-type enzyme Sus scrofa
3.4.19.1 0.68
-
 N-acetyl-Met-Ala mutant enzyme D562N Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.1 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.1 N-acetyl-Met-Ala + H2O
-
 Sus scrofa N-acetyl-Met + Ala
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.19.1 13.4
-
 N-acetyl-Met-Ala mutant enzyme D562N Sus scrofa
3.4.19.1 29
-
 N-acetyl-Met-Ala recombinant wild-type enzyme Sus scrofa
3.4.19.1 33
-
 N-acetyl-Met-Ala wild-type enzyme Sus scrofa