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Literature summary extracted from

  • Kobayashi, K.; Smith, J.A.
    Acyl-peptide hydrolase from rat liver. Characterization of enzyme reaction (1987), J. Biol. Chem., 262, 11435-11445.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.19.1 analysis enzyme is useful for deblocking of peptides Rattus norvegicus

General Stability

EC Number General Stability Organism
3.4.19.1 75% loss of activity in 0.1 M guanidine hydrochloride at 37°C after 200 min Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.1 1-ethyl-3,3'-dimethylaminopropylcarbodiimide
-
 Rattus norvegicus
3.4.19.1 5,5'-dithiobis-(2-nitrobenzoate) partial Rattus norvegicus
3.4.19.1 Cl- activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala Rattus norvegicus
3.4.19.1 DFP
-
 Rattus norvegicus
3.4.19.1 diethyl dicarbonate
-
 Rattus norvegicus
3.4.19.1 Gly methyl ester
-
 Rattus norvegicus
3.4.19.1 iodoacetamide partial Rattus norvegicus
3.4.19.1 iodoacetic acid partial Rattus norvegicus
3.4.19.1 N-acetyl-Ala competitive Rattus norvegicus
3.4.19.1 N-acetyl-Ala chloromethyl ketone inactivation follows first order kinetics, acetyl-Ala protects Rattus norvegicus
3.4.19.1 N-ethyl-5-phenylisoxazolium 3'-sulfonate i.e. Woodward's reagent Rattus norvegicus
3.4.19.1 SCN- activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala Rattus norvegicus
3.4.19.1 Zn2+ most potent inhibitor Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.19.1 Cl- 0.1-0.5 M, activates activity towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide Rattus norvegicus
3.4.19.1 SCN- 0.1-0.5 M, activates towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.1 290000 320000 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.1 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.1 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.1 N-acetyl-Ala p-nitroanilide + H2O
-
 Rattus norvegicus N-acetyl-Ala + p-nitroaniline
-
 ?
3.4.19.1 N-acetyl-Ala-Ala + H2O
-
 Rattus norvegicus N-acetyl-Ala + Ala
-
 ?
3.4.19.1 N-acetyl-Ala-Ala-Ala + H2O
-
 Rattus norvegicus N-acetyl-Ala + Ala-Ala
-
 ?
3.4.19.1 N-acetyl-Ala-Asp + H2O
-
 Rattus norvegicus N-acetyl-Ala + Asp
-
 ?
3.4.19.1 N-acetyl-Ala-beta-naphthylamide + H2O
-
 Rattus norvegicus N-acetyl-Ala + beta-naphthylamine
-
 ?
3.4.19.1 N-acetyl-Ala-Gly + H2O
-
 Rattus norvegicus N-acetyl-Ala + Gly
-
 ?
3.4.19.1 N-acetyl-Ala-Leu + H2O
-
 Rattus norvegicus N-acetyl-Ala + Leu
-
 ?
3.4.19.1 N-acetyl-Ala-Met + H2O
-
 Rattus norvegicus N-acetyl-Ala + Met
-
 ?
3.4.19.1 N-acetyl-Ala-Phe + H2O
-
 Rattus norvegicus N-acetyl-Ala + Phe
-
 ?
3.4.19.1 N-acetyl-Ala-Ser + H2O
-
 Rattus norvegicus N-acetyl-Ala + Ser
-
 ?
3.4.19.1 N-acetyl-Ala-Thr + H2O
-
 Rattus norvegicus N-acetyl-Ala + Thr
-
 ?
3.4.19.1 N-acetyl-Ala-Trp + H2O
-
 Rattus norvegicus N-acetyl-Ala + Trp
-
 ?
3.4.19.1 N-acetyl-Ala-Tyr + H2O
-
 Rattus norvegicus N-acetyl-Ala + Tyr
-
 ?
3.4.19.1 N-acetyl-Gly-Ala + H2O weak activity Rattus norvegicus N-acetyl-Gly + Ala
-
 ?
3.4.19.1 N-acetyl-Leu-Ala + H2O
-
 Rattus norvegicus N-acetyl-Leu + Ala
-
 ?
3.4.19.1 N-acetyl-Met-Ala + H2O
-
 Rattus norvegicus N-acetyl-Met + Ala
-
 ?
3.4.19.1 N-acetyl-Phe-Ala + H2O weak activity Rattus norvegicus N-acetyl-Phe + Ala
-
 ?
3.4.19.1 N-acetyl-Ser-Ala + H2O
-
 Rattus norvegicus N-acetyl-Ser + Ala
-
 ?
3.4.19.1 N-acetyl-Tyr-Ala + H2O weak activity Rattus norvegicus N-acetyl-Tyr + Ala
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.1 7.5
-
 hydrolysis of N-acetyl-Ala p-nitroanilide Rattus norvegicus