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Literature summary extracted from

  • Eisenhauer, D.A.; McDonald, J.K.
    A novel dipeptidyl peptidase II from the porcine ovary. Purification and characterization of a lysosomal serine protease showing enhanced specificity for prolyl bonds (1986), J. Biol. Chem., 261, 8859-8865.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.2 4-Chloromercuriphenylsulfonate
-
 Sus scrofa
3.4.14.2 diisopropyl fluorophosphate
-
 Sus scrofa
3.4.14.2 Hg2+ HgCl2 Sus scrofa
3.4.14.2 iodoacetate weak Sus scrofa
3.4.14.2 Na+ 100 mM NaCl, 11% inhibition Sus scrofa
3.4.14.2 p-Nitrophenyl-p-guanidinobenzoate
-
 Sus scrofa
3.4.14.2 pepstatin 0.01 mM, 11% inhibition Sus scrofa
3.4.14.2 phenylmethylsulfonyl fluoride
-
 Sus scrofa
3.4.14.2 puromycin 1 mM, 47% inhibition Sus scrofa
3.4.14.2 Tris 50 mM, 55% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.2 0.0086
-
 Arg-Pro-2-naphthylamide
-
 Sus scrofa
3.4.14.2 0.045
-
 Phe-Pro-2-naphthylamide
-
 Sus scrofa
3.4.14.2 0.099
-
 Lys-Ala-2-naphthylamide
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.2 lysosome
-
 Sus scrofa 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.2 54000
-
 2 * 54000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.2 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.2 side-chain modification glycoprotein Sus scrofa
3.4.14.2 side-chain modification contains 2% carbohydrate, primarily mannose Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.2
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.2 ovary
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.2 28
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.2 (Ala)3 + H2O
-
 Sus scrofa ?
-
 ?
3.4.14.2 Arg-Ala-2-naphthylamide + H2O 11% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Arg-Ala + 2-naphthylamine
-
 ?
3.4.14.2 Arg-Pro-2-naphthylamide + H2O 27% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Arg-Pro + 2-naphthylamine
-
 ?
3.4.14.2 Gly-Pro-Ala + H2O
-
 Sus scrofa Gly-Pro + Ala
-
 ?
3.4.14.2 Lys-Ala-2-naphthylamide + H2O 15% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Lys-Ala + 2-naphthylamine
-
 ?
3.4.14.2 Lys-Ala-Ala + H2O
-
 Sus scrofa Lys-Ala + Ala
-
 ?
3.4.14.2 Lys-Pro-2-naphthylamide + H2O 46% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Lys-Pro + 2-naphthylamine
-
 ?
3.4.14.2 Phe-Pro-2-naphthylamide + H2O
-
 Sus scrofa Phe-Pro + 2-naphthylamine
-
 ?
3.4.14.2 Phe-Pro-Ala + H2O
-
 Sus scrofa Phe-Pro + Ala
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.2 dimer 2 * 54000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.2 6
-
 Lys-Ala-2-naphthylamide
-
 Sus scrofa
3.4.14.2 6.4
-
 Arg-Pro-2-naphthylamide
-
 Sus scrofa
3.4.14.2 54
-
 Phe-Pro-2-naphthylamide
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.2 5 6
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.2 4
-
 25°C, 24 h, 50% loss of activity Sus scrofa
3.4.14.2 5 7 25°C, 72 h, stable Sus scrofa