Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ichishima, E.; Yamagata, Y.; Chiba, H.; Sawaguchi, K.; Tanaka, T.
    Soluble and bound forms of aminopeptidase in hen's egg yolk (1989), Agric. Biol. Chem., 53, 1867-1872.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline
-
 Gallus gallus
3.4.11.1 bestatin
-
 Gallus gallus
3.4.11.1 puromycin
-
 Gallus gallus
3.4.11.20 1,10-phenanthroline
-
 Gallus gallus
3.4.11.20 bestatin
-
 Gallus gallus
3.4.11.20 EDTA
-
 Gallus gallus
3.4.11.20 OF 4949-II substance produced by Penicillium rugulosum, CAS: [93375-50-9] Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.01
-
 leucine-7-amido-4-methylcoumarin
-
 Gallus gallus
3.4.11.20 0.01
-
 Leu 4-methylcoumarin 7-amide plasma enzyme Gallus gallus
3.4.11.20 0.8
-
 Leu 4-methylcoumarin 7-amide granule enzyme Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytoplasm
-
 Gallus gallus 5737
-
3.4.11.20 cytoplasm low activity Gallus gallus 5737
-
3.4.11.20 cytoplasm 95% of the total activity Gallus gallus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ activation Gallus gallus
3.4.11.20 Co2+ reactivates 1,10-phenanthroline inactivated apoenzyme Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 326000 360000 gel filtration Gallus gallus
3.4.11.20 360000
-
 plasma enzyme, gel filtration Gallus gallus
3.4.11.20 700000
-
 granule enzyme, gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Gallus gallus
-
 hen
-
3.4.11.20 Gallus gallus
-
 hen
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 partially Gallus gallus
3.4.11.20 enzyme form from egg yolk plasma fraction and from granule fraction Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 egg egg yolk Gallus gallus
-
3.4.11.20 egg yolk
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 additional information
-
-
 Gallus gallus
3.4.11.20 additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Leu-p-nitroanilide + H2O Leu substituted by Ala, Gly, Lys Gallus gallus L-leucine + p-nitroaniline
-
 ?
3.4.11.1 Leu-7-amido-4-methylcoumarin + H2O Leu replaced by Arg Gallus gallus leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 Leu 4-methylcoumarin 7-amide + H2O
-
 Gallus gallus ?
-
 ?
3.4.11.20 Leu 4-nitroanilide + H2O
-
 Gallus gallus ?
-
 ?
3.4.11.20 Lys-Ala-Met-Cys-Ala + H2O
-
 Gallus gallus ?
-
 ?
3.4.11.20 Lys-Phe-Ile-Gly-Leu-Met-NH2 + H2O eledoisin-related peptide, lysine, phenylalanine, isoleucine, glycine and leucine are liberated from the aminoterminus, methionine is not released from Met-NH2 of the final product Gallus gallus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 30 50
-
 Gallus gallus
3.4.11.20 30
-
 10 min, pH 7.5, plasma enzyme stable Gallus gallus
3.4.11.20 60
-
 10 min, pH 7.5, 20% loss of plasma enzyme activity, activation of granule enzyme Gallus gallus
3.4.11.20 70
-
 10 min, pH 7.5, complete loss of activity of plasma enzyme, 60% loss of activity of granule enzyme Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 6.5
-
-
 Gallus gallus
3.4.11.20 6.5
-
 plasma enzyme Gallus gallus
3.4.11.20 7.8
-
 granule enzyme Gallus gallus