Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Naseem, I.; Hadi, S.M.
    Single-strand-specific nuclease of pea seeds: glycoprotein nature and associated nucleotidase activity (1987), Arch. Biochem. Biophys., 255, 437-445.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.6 8-hydroxyquinoline
-
 Pisum sativum
3.1.3.6 EDTA
-
 Pisum sativum
3.1.30.1 Co2+ almost complete inhibition of 3'-AMP hydrolysis by 1 mM CoCl2 Pisum sativum
3.1.30.1 EDTA almost complete inhibition of 3'-AMP hydrolysis with 1 mM Pisum sativum
3.1.30.1 Hg2+ complete inhibition of 3'-AMP hydrolysis by 1 mM HgCl2 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.6 Ca2+ stimulation, no absolute requirement Pisum sativum
3.1.3.6 Mg2+ stimulation, no absolute requirement Pisum sativum
3.1.30.1 Ca2+ 1.7fold stimulation of 3'-AMP hydrolysis with 5 mM CaCl2 Pisum sativum
3.1.30.1 Mg2+ 2fold stimulation of 3'-AMP hydrolysis with 5 mM MgCl2 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.6 24000
-
 x * 30200 + x * 24000, SDS-PAGE Pisum sativum
3.1.3.6 30200
-
 x * 30200 + x * 24000, SDS-PAGE Pisum sativum
3.1.30.1 24000
-
 alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE Pisum sativum
3.1.30.1 30200
-
 alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE Pisum sativum
3.1.30.1 54000
-
 native enzyme Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Pisum sativum
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.6 Pisum sativum
-
 a single protein is resonsible for nuclease and 3'-nucleotidase activity
-
3.1.30.1 Pisum sativum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.6 side-chain modification glycoprotein Pisum sativum
3.1.30.1 glycoprotein about 20% carbohydrate Pisum sativum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.6 copurification with nuclease Pisum sativum
3.1.30.1
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.6 seed
-
 Pisum sativum
-
3.1.30.1 seed germinating Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.1 8.3
-
 purified enzyme, DNA as substrate Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.6 3'-AMP + H2O
-
 Pisum sativum adenosine + phosphate
-
 ?
3.1.3.6 additional information no hydrolysis of 5'-AMP Pisum sativum ?
-
 ?
3.1.30.1 DNA + H2O
-
 Pisum sativum 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 dsDNA + H2O higher rate of hydrolysis of alkylated and depurinated dsDNA than native DNA Pisum sativum 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Pisum sativum 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.6 ? x * 30200 + x * 24000, SDS-PAGE Pisum sativum
3.1.30.1 dimer alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.6 60
-
-
 Pisum sativum
3.1.30.1 60
-
 with native DNA or 3'-AMP as substrate Pisum sativum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.6 70
-
 90 min, 90% inactivation Pisum sativum
3.1.30.1 60
-
 after 90 min incubation time 90% of activity towards 3'-AMP and 60% of activity towards DNA lost Pisum sativum