BRENDA - Enzyme Database

Partial purification and properties of an AMP-specific 5'-nucleotidase from pigeon heart

Skladanowski, A.C.; Newby, A.C.; Biochem. J. 268, 117-122 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
ADP
activation of cytosolic enzyme
Columba livia
3.1.3.5
ATP
activation
Columba livia
3.1.3.31
ADP
-
Columba livia
3.1.3.31
additional information
not activated by ATP
Columba livia
Application
EC Number
Application
Commentary
Organism
3.1.3.5
pharmacology
potential site for pharmacological intervention to protect organs against nucleotide depletion
Columba livia
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
4-nitrophenyl phosphate
-
Columba livia
3.1.3.5
adenosine 5'-(alpha,beta-methylene)diphosphate
not inhibitory
Columba livia
3.1.3.31
4-nitrophenyl phosphate
49.9% inhibition at 10 mM
Columba livia
3.1.3.31
5'-deoxy-5'-isobutylthioadenosine
21.1% inhibition at 3 mM
Columba livia
3.1.3.31
5'-deoxy-5'-isobutylthioinosine
54.8% inhibition at 7 mM
Columba livia
3.1.3.31
CMP
55.4% inhibition at 10 mM
Columba livia
3.1.3.31
D-ribose 5-phosphate
14.2% inhibition at 10 mM
Columba livia
3.1.3.31
dAMP
66.1% inhibition at 10 mM
Columba livia
3.1.3.31
GMP
42.7% inhibition at 10 mM
Columba livia
3.1.3.31
IMP
36% inhibition at 10 mM
Columba livia
3.1.3.31
Mg2+
inhibitory at 10 mM and above
Columba livia
3.1.3.31
additional information
not inhibited by [alpha,beta-methylene]ADP
Columba livia
3.1.3.31
phosphate
31.7% inhibition at 20 mM
Columba livia
3.1.3.31
UMP
74.5% inhibition at 10 mM
Columba livia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
4.7
-
5'-AMP
-
Columba livia
3.1.3.5
7.7
-
5'-IMP
-
Columba livia
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytoplasm
-
Columba livia
5737
-
3.1.3.31
soluble
-
Columba livia
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Mg2+
activation
Columba livia
3.1.3.31
Mg2+
required for activity
Columba livia
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
150000
-
gel filtration
Columba livia
3.1.3.31
150000
-
gel filtration
Columba livia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
5'-IMP + H2O
Columba livia
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
?
-
-
-
3.1.3.31
AMP + H2O
Columba livia
100% activity, 15fold preference for AMP over IMP
adenosine + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Columba livia
-
pigeon
-
3.1.3.31
Columba livia
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.31
ammonium sulfate precipitation, phosphocellulose column chromatography, and UltraPak TSK G4000gel filtration
Columba livia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
heart
-
Columba livia
-
3.1.3.31
heart
-
Columba livia
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
35.8
-
-
Columba livia
3.1.3.31
0.17
-
crude enzyme from supernatant, at pH 6.5 and 37°C
Columba livia
3.1.3.31
35.83
-
after 211fold purification, at pH 6.5 and 37°C
Columba livia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95065
Columba livia
adenosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-CMP + H2O
61.7% of the activity with 5'-AMP
95065
Columba livia
cytidine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95065
Columba livia
deoxyadenosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dAMP + H2O
66% of the activity with 5'-AMP
95065
Columba livia
deoxyadenosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95065
Columba livia
deoxyguanosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dGMP + H2O
49.9% of the activity with 5'-AMP
95065
Columba livia
deoxyguanosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dIMP + H2O
i.e. 2'-deoxyinosine 5'-phosphate
95065
Columba livia
deoxyinosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dIMP + H2O
60.9% of the activity with 5'-AMP
95065
Columba livia
deoxyinosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-GMP + H2O
33.5% of the activity with 5'-AMP
95065
Columba livia
guanosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-IMP + H2O
33.1% of the activity with 5'-AMP
95065
Columba livia
inosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
95065
Columba livia
?
-
-
-
-
3.1.3.5
5'-TMP + H2O
15.2% of the activity with 5'-AMP
95065
Columba livia
thymidine + phosphate
-
-
-
-
3.1.3.5
5'-UMP + H2O
49.5% of the activity with 5'-AMP
95065
Columba livia
uridine + phosphate
-
95065
Columba livia
?
3.1.3.31
AMP + H2O
100% activity, 15fold preference for AMP over IMP
95065
Columba livia
adenosine + phosphate
-
-
-
?
3.1.3.31
CMP + H2O
61.7% activity compared to AMP
95065
Columba livia
cytosine + phosphate
-
-
-
?
3.1.3.31
dAMP + H2O
66% activity compared to AMP
95065
Columba livia
deoxyadenosine + phosphate
-
-
-
?
3.1.3.31
dCMP + H2O
12.1% activity compared to AMP
95065
Columba livia
deoxycytosine + phosphate
-
-
-
?
3.1.3.31
dGMP + H2O
49.9% activity compared to AMP
95065
Columba livia
deoxyguanosine + phosphate
-
-
-
?
3.1.3.31
dIMP + H2O
60.9% activity compared to AMP
95065
Columba livia
deoxyinosine + phosphate
-
-
-
?
3.1.3.31
GMP + H2O
33.5% activity compared to AMP
95065
Columba livia
guanosine + phosphate
-
-
-
?
3.1.3.31
IMP + H2O
33.1% activity compared to AMP
95065
Columba livia
inosine + phosphate
-
-
-
?
3.1.3.31
additional information
very low rates of dephosphorylation with beta-glycerophosphate, D-ribose 5-phosphate and 4-nitrophenyl phosphate, while 3',5'-cyclic AMP is not hydrolysed significantly
95065
Columba livia
?
-
-
-
-
3.1.3.31
TMP + H2O
15.2% activity compared to AMP
95065
Columba livia
thymidine + phosphate
-
-
-
?
3.1.3.31
UMP + H2O
49.5% activity compared to AMP
95065
Columba livia
uridine + phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.5
7
-
-
Columba livia
3.1.3.31
7
-
-
Columba livia
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
ADP
activation of cytosolic enzyme
Columba livia
3.1.3.5
ATP
activation
Columba livia
3.1.3.31
ADP
-
Columba livia
3.1.3.31
additional information
not activated by ATP
Columba livia
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.3.5
pharmacology
potential site for pharmacological intervention to protect organs against nucleotide depletion
Columba livia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
4-nitrophenyl phosphate
-
Columba livia
3.1.3.5
adenosine 5'-(alpha,beta-methylene)diphosphate
not inhibitory
Columba livia
3.1.3.31
4-nitrophenyl phosphate
49.9% inhibition at 10 mM
Columba livia
3.1.3.31
5'-deoxy-5'-isobutylthioadenosine
21.1% inhibition at 3 mM
Columba livia
3.1.3.31
5'-deoxy-5'-isobutylthioinosine
54.8% inhibition at 7 mM
Columba livia
3.1.3.31
CMP
55.4% inhibition at 10 mM
Columba livia
3.1.3.31
D-ribose 5-phosphate
14.2% inhibition at 10 mM
Columba livia
3.1.3.31
dAMP
66.1% inhibition at 10 mM
Columba livia
3.1.3.31
GMP
42.7% inhibition at 10 mM
Columba livia
3.1.3.31
IMP
36% inhibition at 10 mM
Columba livia
3.1.3.31
Mg2+
inhibitory at 10 mM and above
Columba livia
3.1.3.31
additional information
not inhibited by [alpha,beta-methylene]ADP
Columba livia
3.1.3.31
phosphate
31.7% inhibition at 20 mM
Columba livia
3.1.3.31
UMP
74.5% inhibition at 10 mM
Columba livia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
4.7
-
5'-AMP
-
Columba livia
3.1.3.5
7.7
-
5'-IMP
-
Columba livia
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytoplasm
-
Columba livia
5737
-
3.1.3.31
soluble
-
Columba livia
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Mg2+
activation
Columba livia
3.1.3.31
Mg2+
required for activity
Columba livia
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
150000
-
gel filtration
Columba livia
3.1.3.31
150000
-
gel filtration
Columba livia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
5'-IMP + H2O
Columba livia
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
?
-
-
-
3.1.3.31
AMP + H2O
Columba livia
100% activity, 15fold preference for AMP over IMP
adenosine + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.31
ammonium sulfate precipitation, phosphocellulose column chromatography, and UltraPak TSK G4000gel filtration
Columba livia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
heart
-
Columba livia
-
3.1.3.31
heart
-
Columba livia
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
35.8
-
-
Columba livia
3.1.3.31
0.17
-
crude enzyme from supernatant, at pH 6.5 and 37°C
Columba livia
3.1.3.31
35.83
-
after 211fold purification, at pH 6.5 and 37°C
Columba livia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95065
Columba livia
adenosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-CMP + H2O
61.7% of the activity with 5'-AMP
95065
Columba livia
cytidine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95065
Columba livia
deoxyadenosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dAMP + H2O
66% of the activity with 5'-AMP
95065
Columba livia
deoxyadenosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95065
Columba livia
deoxyguanosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dGMP + H2O
49.9% of the activity with 5'-AMP
95065
Columba livia
deoxyguanosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dIMP + H2O
i.e. 2'-deoxyinosine 5'-phosphate
95065
Columba livia
deoxyinosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-dIMP + H2O
60.9% of the activity with 5'-AMP
95065
Columba livia
deoxyinosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-GMP + H2O
33.5% of the activity with 5'-AMP
95065
Columba livia
guanosine + phosphate
-
95065
Columba livia
?
3.1.3.5
5'-IMP + H2O
33.1% of the activity with 5'-AMP
95065
Columba livia
inosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
95065
Columba livia
?
-
-
-
-
3.1.3.5
5'-TMP + H2O
15.2% of the activity with 5'-AMP
95065
Columba livia
thymidine + phosphate
-
-
-
-
3.1.3.5
5'-UMP + H2O
49.5% of the activity with 5'-AMP
95065
Columba livia
uridine + phosphate
-
95065
Columba livia
?
3.1.3.31
AMP + H2O
100% activity, 15fold preference for AMP over IMP
95065
Columba livia
adenosine + phosphate
-
-
-
?
3.1.3.31
CMP + H2O
61.7% activity compared to AMP
95065
Columba livia
cytosine + phosphate
-
-
-
?
3.1.3.31
dAMP + H2O
66% activity compared to AMP
95065
Columba livia
deoxyadenosine + phosphate
-
-
-
?
3.1.3.31
dCMP + H2O
12.1% activity compared to AMP
95065
Columba livia
deoxycytosine + phosphate
-
-
-
?
3.1.3.31
dGMP + H2O
49.9% activity compared to AMP
95065
Columba livia
deoxyguanosine + phosphate
-
-
-
?
3.1.3.31
dIMP + H2O
60.9% activity compared to AMP
95065
Columba livia
deoxyinosine + phosphate
-
-
-
?
3.1.3.31
GMP + H2O
33.5% activity compared to AMP
95065
Columba livia
guanosine + phosphate
-
-
-
?
3.1.3.31
IMP + H2O
33.1% activity compared to AMP
95065
Columba livia
inosine + phosphate
-
-
-
?
3.1.3.31
additional information
very low rates of dephosphorylation with beta-glycerophosphate, D-ribose 5-phosphate and 4-nitrophenyl phosphate, while 3',5'-cyclic AMP is not hydrolysed significantly
95065
Columba livia
?
-
-
-
-
3.1.3.31
TMP + H2O
15.2% activity compared to AMP
95065
Columba livia
thymidine + phosphate
-
-
-
?
3.1.3.31
UMP + H2O
49.5% activity compared to AMP
95065
Columba livia
uridine + phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.5
7
-
-
Columba livia
3.1.3.31
7
-
-
Columba livia