BRENDA - Enzyme Database

Nucleotide and nucleoside analogues as inhibitors of cytosolic 5'-nucleotidase I from heart

Garvey, E.P.; Lowen, G.T.; Almond, M.R.; Biochemistry 37, 9043-9051 (1998)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.1.3.5
pharmacology
inhibitors could be used to diminish nucleotide depletion during ischemia/reperfusion
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
(2R,4S,5R)-1-[5-(2-fluoro-2-phosphonoethyl)tetrahydro-4-hydroxy-2-furyl]thymine
-
Oryctolagus cuniculus
3.1.3.5
(2R,4S,5R)-1-[tetrahydro-4-hydroxy-5-(2-phosphonoethyl)-2-furyl]thymine
-
Oryctolagus cuniculus
3.1.3.5
(2R,5R)-1-[5-(2-fluoro-2-phosphonoethyl)tetrahydro-2-furyl]thymine
-
Oryctolagus cuniculus
3.1.3.5
(2R,5S)-1-[tetrahydro-5-(2-phosphonoethyl)-2-furyl]thymine
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.02
-
5'-TMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.061
-
5'-dCMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.11
-
5'-CMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.13
-
5'-dAMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.32
-
5'-GMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
1
-
5'-IMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
1.2
-
5'-AMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytosol
-
Oryctolagus cuniculus
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Oryctolagus cuniculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
heart
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
10
-
enzyme e-N
Oryctolagus cuniculus
3.1.3.5
71
-
enzyme c-N-I
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95058
Oryctolagus cuniculus
adenosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-CMP + H2O
-
95058
Oryctolagus cuniculus
cytidine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95058
Oryctolagus cuniculus
deoxyadenosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95058
Oryctolagus cuniculus
deoxycytidine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95058
Oryctolagus cuniculus
deoxyguanosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-GMP + H2O
-
95058
Oryctolagus cuniculus
guanosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-IMP + H2O
-
95058
Oryctolagus cuniculus
inosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-TMP + H2O
-
95058
Oryctolagus cuniculus
thymidine + phosphate
-
-
-
-
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.3.5
pharmacology
inhibitors could be used to diminish nucleotide depletion during ischemia/reperfusion
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
(2R,4S,5R)-1-[5-(2-fluoro-2-phosphonoethyl)tetrahydro-4-hydroxy-2-furyl]thymine
-
Oryctolagus cuniculus
3.1.3.5
(2R,4S,5R)-1-[tetrahydro-4-hydroxy-5-(2-phosphonoethyl)-2-furyl]thymine
-
Oryctolagus cuniculus
3.1.3.5
(2R,5R)-1-[5-(2-fluoro-2-phosphonoethyl)tetrahydro-2-furyl]thymine
-
Oryctolagus cuniculus
3.1.3.5
(2R,5S)-1-[tetrahydro-5-(2-phosphonoethyl)-2-furyl]thymine
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.02
-
5'-TMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.061
-
5'-dCMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.11
-
5'-CMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.13
-
5'-dAMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
0.32
-
5'-GMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
1
-
5'-IMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
3.1.3.5
1.2
-
5'-AMP
cytosolic enzyme c-N-I
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytosol
-
Oryctolagus cuniculus
5829
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
heart
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
10
-
enzyme e-N
Oryctolagus cuniculus
3.1.3.5
71
-
enzyme c-N-I
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95058
Oryctolagus cuniculus
adenosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-CMP + H2O
-
95058
Oryctolagus cuniculus
cytidine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
95058
Oryctolagus cuniculus
deoxyadenosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95058
Oryctolagus cuniculus
deoxycytidine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
95058
Oryctolagus cuniculus
deoxyguanosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-GMP + H2O
-
95058
Oryctolagus cuniculus
guanosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-IMP + H2O
-
95058
Oryctolagus cuniculus
inosine + phosphate
-
95058
Oryctolagus cuniculus
?
3.1.3.5
5'-TMP + H2O
-
95058
Oryctolagus cuniculus
thymidine + phosphate
-
-
-
-