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Literature summary extracted from

  • Gupta, A.; Sharma, C.B.
    Purification to homogeneity and characterization of plasma membrane and Golgi apparatus-specific 5'-adenosine monophosphatases from peanut cotyledons (1996), Plant Sci., 117, 65-74.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.58
-
 5'-AMP enzyme from plasma membrane Arachis hypogaea
3.1.3.5 1.02
-
 5'-AMP enzyme from Golgi apparatus Arachis hypogaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 Golgi apparatus
-
 Arachis hypogaea 5794
-
3.1.3.5 plasma membrane
-
 Arachis hypogaea 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 26300
-
 x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE Arachis hypogaea
3.1.3.5 52400
-
 x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE Arachis hypogaea

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Arachis hypogaea
-
 peanut
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 side-chain modification enzymes from plasma membrane and Golgi apparatus have 37% and 39% carbohydrate, respectively Arachis hypogaea

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 enzyme from plasma membrane and enzyme from Golgi apparatus Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 cotyledon
-
 Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 182
-
 enzyme from Golgi apparatus Arachis hypogaea
3.1.3.5 589.4
-
 enzyme from plasma membrane Arachis hypogaea

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -20°C, 24 h, 80-90% loss of activity Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O best substrate for enzyme from plasma membrane and Golgi apparatus Arachis hypogaea adenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O plasma membrane enzyme: 2.85% of the activity with 5'-AMP, Golgi apparatus enzyme: 46% of the activity with 5'-AMP Arachis hypogaea guanosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O plasma membrane enzyme: 7.5% of the activity with 5'-AMP, Golgi apparatus enzyme: 16.2% of the activity with 5'-AMP Arachis hypogaea uridine + phosphate
-
 ?
3.1.3.5 p-nitrophenylphosphate + H2O plasma membrane enzyme: 1% of the activity with 5'-AMP, Golgi apparatus enzyme: 39% of the activity with 5'-AMP Arachis hypogaea p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 GA-AMPase
-
 Arachis hypogaea
3.1.3.5 PM-AMPase
-
 Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 5 5.5 enzymes from plasma membrane and Golgi apparatus Arachis hypogaea