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Literature summary extracted from

  • Gibson, W.B.; Drummond, G.I.
    Properties of 5'-nucleotidase from avian heart (1972), Biochemistry, 11, 223-229.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 ATP
-
 Gallus gallus
3.1.3.5 ATP activation at substrate concentration below 6 mM Columba livia

General Stability

EC Number General Stability Organism
3.1.3.5 stabilization by KCl and ethylene glycol Columba livia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 2'-AMP
-
 Columba livia
3.1.3.5 3'-AMP
-
 Columba livia
3.1.3.5 ATP inhibition at substrate concentration above 6 mM Columba livia
3.1.3.5 ATP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 12
-
 5'-AMP
-
 Columba livia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytoplasm
-
 Gallus gallus 5737
-
3.1.3.5 cytoplasm
-
 Rattus norvegicus 5737
-
3.1.3.5 cytoplasm
-
 Columba livia 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+
-
 Rattus norvegicus
3.1.3.5 Mg2+ activation Gallus gallus
3.1.3.5 Mg2+ activation Columba livia

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Columba livia
-
 pigeon
-
3.1.3.5 Gallus gallus
-
-
-
3.1.3.5 Rattus norvegicus
-
-
-
3.1.3.5 Sturnus vulgaris
-
 starling
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 partially Columba livia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 heart
-
 Rattus norvegicus
-
3.1.3.5 heart
-
 Columba livia
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -80°C, semipurified solution, 6 weeks stable Columba livia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Gallus gallus adenosine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O
-
 Columba livia adenosine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O
-
 Sturnus vulgaris adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Gallus gallus cytidine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Rattus norvegicus cytidine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Columba livia cytidine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Sturnus vulgaris cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Gallus gallus guanosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Rattus norvegicus guanosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Columba livia guanosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Sturnus vulgaris guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Gallus gallus inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Rattus norvegicus inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Columba livia inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Sturnus vulgaris inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Gallus gallus uridine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Rattus norvegicus uridine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Columba livia uridine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Sturnus vulgaris uridine + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7
-
-
 Columba livia